OPT3_ARATH
ID OPT3_ARATH Reviewed; 737 AA.
AC O23482; F4JLS9; Q8H0V0; Q940I5;
DT 07-JUN-2005, integrated into UniProtKB/Swiss-Prot.
DT 03-MAY-2011, sequence version 3.
DT 25-MAY-2022, entry version 127.
DE RecName: Full=Oligopeptide transporter 3;
DE Short=AtOPT3;
GN Name=OPT3; OrderedLocusNames=At4g16370; ORFNames=dl4215c, FCAALL.72;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9461215; DOI=10.1038/35140;
RA Bevan M., Bancroft I., Bent E., Love K., Goodman H.M., Dean C.,
RA Bergkamp R., Dirkse W., van Staveren M., Stiekema W., Drost L., Ridley P.,
RA Hudson S.-A., Patel K., Murphy G., Piffanelli P., Wedler H., Wedler E.,
RA Wambutt R., Weitzenegger T., Pohl T., Terryn N., Gielen J., Villarroel R.,
RA De Clercq R., van Montagu M., Lecharny A., Aubourg S., Gy I., Kreis M.,
RA Lao N., Kavanagh T., Hempel S., Kotter P., Entian K.-D., Rieger M.,
RA Schaefer M., Funk B., Mueller-Auer S., Silvey M., James R., Monfort A.,
RA Pons A., Puigdomenech P., Douka A., Voukelatou E., Milioni D.,
RA Hatzopoulos P., Piravandi E., Obermaier B., Hilbert H., Duesterhoeft A.,
RA Moores T., Jones J.D.G., Eneva T., Palme K., Benes V., Rechmann S.,
RA Ansorge W., Cooke R., Berger C., Delseny M., Voet M., Volckaert G.,
RA Mewes H.-W., Klosterman S., Schueller C., Chalwatzis N.;
RT "Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis
RT thaliana.";
RL Nature 391:485-488(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [3]
RP GENOME REANNOTATION, AND SEQUENCE REVISION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP FUNCTION, NOMENCLATURE, AND TISSUE SPECIFICITY.
RX PubMed=11788749; DOI=10.1104/pp.010332;
RA Koh S., Wiles A.M., Sharp J.S., Naider F.R., Becker J.M., Stacey G.;
RT "An oligopeptide transporter gene family in Arabidopsis.";
RL Plant Physiol. 128:21-29(2002).
RN [6]
RP FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=12417702; DOI=10.1105/tpc.005629;
RA Stacey M.G., Koh S., Becker J., Stacey G.;
RT "AtOPT3, a member of the oligopeptide transporter family, is essential for
RT embryo development in Arabidopsis.";
RL Plant Cell 14:2799-2811(2002).
RN [7]
RP FUNCTION, AND INDUCTION.
RX PubMed=13129917; DOI=10.1074/jbc.m309338200;
RA Wintz H., Fox T., Wu Y.-Y., Feng V., Chen W., Chang H.-S., Zhu T.,
RA Vulpe C.D.;
RT "Expression profiles of Arabidopsis thaliana in mineral deficiencies reveal
RT novel transporters involved in metal homeostasis.";
RL J. Biol. Chem. 278:47644-47653(2003).
CC -!- FUNCTION: May be involved in the translocation of tetra- and
CC pentapeptides across the cellular membrane in an energy-dependent
CC manner. Acts also as a metal transporter that could be a component of
CC the copper transport machinery. Essential for early embryo development.
CC {ECO:0000269|PubMed:11788749, ECO:0000269|PubMed:12417702,
CC ECO:0000269|PubMed:13129917}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Strong expression in flowers, leaves and roots.
CC Preferentially expressed in the vascular tissues of seedlings and
CC mature plants as well as in pollen and developing embryos.
CC {ECO:0000269|PubMed:11788749, ECO:0000269|PubMed:12417702}.
CC -!- DEVELOPMENTAL STAGE: Expressed 2 to 4 hours after fertilization in the
CC embryo sac and subsequently in developing maternal tissues. By the
CC globular stage, expression is observed in the developing endosperm,
CC integument layers, the embryo proper, and the suspensor of the
CC developing embryo. From the heart stage onward, expression observed in
CC the embryo but neither in the suspensor nor in the endosperm and
CC integument tissues. {ECO:0000269|PubMed:12417702}.
CC -!- INDUCTION: Highly induced by iron, copper and manganese deficiencies.
CC {ECO:0000269|PubMed:13129917}.
CC -!- SIMILARITY: Belongs to the oligopeptide OPT transporter (TC 2.A.67.1)
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB10414.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB78679.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; Z97341; CAB10414.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161543; CAB78679.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE83738.1; -; Genomic_DNA.
DR EMBL; BT002023; AAN72034.1; -; mRNA.
DR EMBL; AY054590; AAK96781.1; -; mRNA.
DR PIR; D71430; D71430.
DR RefSeq; NP_567493.5; NM_117732.7.
DR AlphaFoldDB; O23482; -.
DR STRING; 3702.AT4G16370.1; -.
DR TCDB; 2.A.67.1.7; the oligopeptide transporter (opt) family.
DR PaxDb; O23482; -.
DR PRIDE; O23482; -.
DR ProteomicsDB; 248817; -.
DR GeneID; 827332; -.
DR KEGG; ath:AT4G16370; -.
DR Araport; AT4G16370; -.
DR TAIR; locus:2130529; AT4G16370.
DR eggNOG; KOG2262; Eukaryota.
DR HOGENOM; CLU_004965_0_1_1; -.
DR InParanoid; O23482; -.
DR OrthoDB; 190227at2759; -.
DR PRO; PR:O23482; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; O23482; baseline and differential.
DR Genevisible; O23482; AT.
DR GO; GO:0005829; C:cytosol; HDA:TAIR.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0016020; C:membrane; ISS:TAIR.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR GO; GO:0035673; F:oligopeptide transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0046915; F:transition metal ion transmembrane transporter activity; IDA:TAIR.
DR GO; GO:0006875; P:cellular metal ion homeostasis; IMP:TAIR.
DR GO; GO:0055072; P:iron ion homeostasis; IDA:TAIR.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:1990388; P:xylem-to-phloem iron transport; IEP:TAIR.
DR InterPro; IPR004648; Oligpept_transpt.
DR InterPro; IPR004813; OPT.
DR PANTHER; PTHR22601; PTHR22601; 1.
DR Pfam; PF03169; OPT; 1.
DR TIGRFAMs; TIGR00727; ISP4_OPT; 1.
DR TIGRFAMs; TIGR00728; OPT_sfam; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; Membrane; Peptide transport; Protein transport;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..737
FT /note="Oligopeptide transporter 3"
FT /id="PRO_0000213780"
FT TRANSMEM 45..65
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 69..89
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 117..137
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 153..173
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 215..235
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 255..275
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 289..309
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 357..377
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 418..438
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 446..466
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 478..498
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 532..552
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 604..624
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 629..649
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 650..670
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 681..701
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 45
FT /note="A -> T (in Ref. 4; AAK96781)"
FT /evidence="ECO:0000305"
FT CONFLICT 81
FT /note="V -> G (in Ref. 1; CAB10414 and 2; CAB78679)"
FT /evidence="ECO:0000305"
FT CONFLICT 239
FT /note="F -> S (in Ref. 1; CAB10414 and 2; CAB78679)"
FT /evidence="ECO:0000305"
FT CONFLICT 322
FT /note="F -> S (in Ref. 1; CAB10414 and 2; CAB78679)"
FT /evidence="ECO:0000305"
FT CONFLICT 527
FT /note="R -> P (in Ref. 1; CAB10414 and 2; CAB78679)"
FT /evidence="ECO:0000305"
FT CONFLICT 605..606
FT /note="WL -> GF (in Ref. 1; CAB10414 and 2; CAB78679)"
FT /evidence="ECO:0000305"
FT CONFLICT 605
FT /note="W -> G (in Ref. 3; AEE83738)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 737 AA; 82441 MW; 8E3A03673C0C12D6 CRC64;
MDAEKATDKT NVHLSSDHER CPVEEVALVV PETDDPSLPV MTFRAWFLGL TSCVLLIFLN
TFFTYRTQPL TISAILMQIA VLPIGKFMAR TLPTTSHNLL GWSFSLNPGP FNIKEHVIIT
IFANCGVAYG GGDAYSIGAI TVMKAYYKQS LSFICGLFIV LTTQILGYGW AGILRRYLVD
PVDMWWPSNL AQVSLFRALH EKENKSKGLT RMKFFLVALG ASFIYYALPG YLFPILTFFS
WVCWAWPNSI TAQQVGSGYH GLGVGAFTLD WAGISAYHGS PLVAPWSSIL NVGVGFIMFI
YIIVPVCYWK FNTFDARKFP IFSNQLFTTS GQKYDTTKIL TPQFDLDIGA YNNYGKLYLS
PLFALSIGSG FARFTATLTH VALFNGRDIW KQTWSAVNTT KLDIHGKLMQ SYKKVPEWWF
YILLAGSVAM SLLMSFVWKE SVQLPWWGML FAFALAFIVT LPIGVIQATT NQQPGYDIIG
QFIIGYILPG KPIANLIFKI YGRISTVHAL SFLADLKLGH YMKIPPRCMY TAQLVGTVVA
GVVNLGVAWW MLESIQDICD IEGDHPNSPW TCPKYRVTFD ASVIWGLIGP RRLFGPGGMY
RNLVWLFLIG AVLPVPVWAL SKIFPNKKWI PLINIPVISY GFAGMPPATP TNIASWLVTG
TIFNYFVFNY HKRWWQKYNY VLSAALDAGT AFMGVLLFFA LQNAGHDLKW WGTEVDHCPL
ASCPTAPGIK AKGCPVF
