OPTIX_DROME
ID OPTIX_DROME Reviewed; 487 AA.
AC Q95RW8; O76291; Q8MKN3; Q9UA64; Q9V355;
DT 27-JAN-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Protein Optix;
DE AltName: Full=Homeobox protein SIX3;
DE Short=D-Six3;
GN Name=Optix; Synonyms=opt, Six3; ORFNames=CG18455;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|EMBL:AAL28625.1};
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B), FUNCTION, TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RC STRAIN=Canton-S; TISSUE=Larva;
RX PubMed=10381573; DOI=10.1016/s0925-4773(99)00045-3;
RA Seo H.-C., Curtiss J., Mlodzik M., Fjose A.;
RT "Six class homeobox genes in Drosophila belong to three distinct families
RT and are involved in head development.";
RL Mech. Dev. 83:127-139(1999).
RN [2] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM C).
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M.,
RA Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A.,
RA Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A.,
RA Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S.,
RA Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E.;
RL Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN [6] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [MRNA] OF 155-214 (ISOFORMS A/C), FUNCTION, TISSUE
RP SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=9724757; DOI=10.1073/pnas.95.18.10643;
RA Toy J., Yang J.-M., Leppert G.S., Sundin O.H.;
RT "The optx2 homeobox gene is expressed in early precursors of the eye and
RT activates retina-specific genes.";
RL Proc. Natl. Acad. Sci. U.S.A. 95:10643-10648(1998).
CC -!- FUNCTION: May be involved in head or eye development; development of
CC the clypeolabrum and several head sensory organs.
CC {ECO:0000269|PubMed:10381573, ECO:0000269|PubMed:9724757}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=A;
CC IsoId=Q95RW8-1; Sequence=Displayed;
CC Name=B;
CC IsoId=Q95RW8-2; Sequence=VSP_002258;
CC Name=C;
CC IsoId=Q95RW8-3; Sequence=VSP_002259;
CC -!- TISSUE SPECIFICITY: Expressed during early development of the head.
CC First expressed in a band around the anterior end of stage 5 blastoderm
CC embryo, at 93% to 85% egg length. By gastrula stage, site of expression
CC shifts to the dorsal-anterior region. At stage 12, expression is found
CC in the clypeolabrum, the stomodaeum, and in ectoderm dorsal to the
CC future supraesophageal ganglion. {ECO:0000269|PubMed:10381573,
CC ECO:0000269|PubMed:9724757}.
CC -!- DEVELOPMENTAL STAGE: Expressed during embryonic development.
CC {ECO:0000269|PubMed:10381573, ECO:0000269|PubMed:9724757}.
CC -!- SIMILARITY: Belongs to the SIX/Sine oculis homeobox family.
CC {ECO:0000305}.
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DR EMBL; AF099184; AAD39863.1; -; mRNA.
DR EMBL; AE013599; AAF59147.3; -; Genomic_DNA.
DR EMBL; AE013599; AAM68882.1; -; Genomic_DNA.
DR EMBL; AY061077; AAL28625.1; -; mRNA.
DR EMBL; BT003802; AAO41485.1; -; mRNA.
DR EMBL; AF050132; AAC33852.1; -; mRNA.
DR RefSeq; NP_524695.2; NM_079956.3. [Q95RW8-1]
DR RefSeq; NP_724640.1; NM_165584.2. [Q95RW8-3]
DR AlphaFoldDB; Q95RW8; -.
DR SMR; Q95RW8; -.
DR BioGRID; 68835; 8.
DR IntAct; Q95RW8; 3.
DR STRING; 7227.FBpp0087941; -.
DR PaxDb; Q95RW8; -.
DR DNASU; 44108; -.
DR EnsemblMetazoa; FBtr0088865; FBpp0087941; FBgn0025360. [Q95RW8-1]
DR EnsemblMetazoa; FBtr0088866; FBpp0087942; FBgn0025360. [Q95RW8-3]
DR GeneID; 44108; -.
DR KEGG; dme:Dmel_CG18455; -.
DR UCSC; CG18455-RA; d. melanogaster. [Q95RW8-1]
DR CTD; 44108; -.
DR FlyBase; FBgn0025360; Optix.
DR VEuPathDB; VectorBase:FBgn0025360; -.
DR eggNOG; KOG0775; Eukaryota.
DR GeneTree; ENSGT00940000169263; -.
DR HOGENOM; CLU_032169_0_0_1; -.
DR InParanoid; Q95RW8; -.
DR OMA; NRIQHNQ; -.
DR OrthoDB; 1040044at2759; -.
DR PhylomeDB; Q95RW8; -.
DR SignaLink; Q95RW8; -.
DR BioGRID-ORCS; 44108; 0 hits in 3 CRISPR screens.
DR ChiTaRS; Optix; fly.
DR GenomeRNAi; 44108; -.
DR PRO; PR:Q95RW8; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0025360; Expressed in labral segment and 73 other tissues.
DR ExpressionAtlas; Q95RW8; baseline and differential.
DR Genevisible; Q95RW8; DM.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005667; C:transcription regulator complex; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:FlyBase.
DR GO; GO:0048749; P:compound eye development; IMP:FlyBase.
DR GO; GO:0001745; P:compound eye morphogenesis; IMP:FlyBase.
DR GO; GO:0001751; P:compound eye photoreceptor cell differentiation; IMP:FlyBase.
DR GO; GO:0048813; P:dendrite morphogenesis; IMP:FlyBase.
DR GO; GO:0007458; P:progression of morphogenetic furrow involved in compound eye morphogenesis; IMP:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR031701; SIX1_SD.
DR Pfam; PF00046; Homeodomain; 1.
DR Pfam; PF16878; SIX1_SD; 1.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Developmental protein; DNA-binding; Homeobox;
KW Nucleus; Reference proteome.
FT CHAIN 1..487
FT /note="Protein Optix"
FT /id="PRO_0000049202"
FT DNA_BIND 154..214
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 182..330
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 443..463
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 240..280
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 304..318
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..228
FT /note="Missing (in isoform C)"
FT /evidence="ECO:0000303|Ref.5"
FT /id="VSP_002259"
FT VAR_SEQ 221..382
FT /note="Missing (in isoform B)"
FT /evidence="ECO:0000303|PubMed:10381573"
FT /id="VSP_002258"
FT CONFLICT 446
FT /note="G -> R (in Ref. 1; AAD39863)"
FT /evidence="ECO:0000305"
FT CONFLICT 455..487
FT /note="Missing (in Ref. 1; AAD39863)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 487 AA; 52419 MW; ECA9FD59F171E599 CRC64;
MAVGPTEGKQ PPSESFSPTH HQIIAPSPIL AVPTLAFSAA QVEIVCKTLE DSGDIERLAR
FLWSLPVALP NMHEILNCEA VLRARAVVAY HVGNFRELYA IIENHKFTKA SYGKLQAMWL
EAHYIEAEKL RGRSLGPVDK YRVRKKFPLP PTIWDGEQKT HCFKERTRSL LREWYLQDPY
PNPTKKRELA KATGLNPTQV GNWFKNRRQR DRAAAAKNRI QHSQNSSGMG CRSRRADGAA
SPTPSDSSDS DISLGTHSPV PSSLQLQHSP GSTSNGANDR EESLSVDDDK PRDLSGSLPL
PLSLPLPLAS PTHTPPQLPP GYGGGAGAGP GGPLTGPGCL PPFKLDAATS LFSAGCYLQS
FSNLKEMSQQ FPIQPIVLRP HPQLPQSLAL NGASGGPPLH HPAYAAAYSV ECVPGGHGPP
HPPPKLRINS PEKLNSTAVA AAASVGGGGG NQHHEPTTTG YHHSGQLMLH RPFSTSPELK
HSAPEIT
