ID   OPUAA_LACLA             Reviewed;         408 AA.
AC   Q9KIF7;
DT   30-AUG-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 126.
DE   RecName: Full=Glycine betaine transport ATP-binding protein OpuAA;
DE            EC=7.6.2.9;
DE   AltName: Full=Quaternary-amine-transporting ATPase;
GN   Name=opuAA; Synonyms=busAA; OrderedLocusNames=LL1452; ORFNames=L74195;
OS   Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
OC   Lactococcus.
OX   NCBI_TaxID=272623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=10860977; DOI=10.1073/pnas.97.13.7102;
RA   van der Heide T., Poolman B.;
RT   "Osmoregulated ABC-transport system of Lactococcus lactis senses water
RT   stress via changes in the physical state of the membrane.";
RL   Proc. Natl. Acad. Sci. U.S.A. 97:7102-7106(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=IL1403;
RX   PubMed=11337471; DOI=10.1101/gr.gr-1697r;
RA   Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J.,
RA   Ehrlich S.D., Sorokin A.;
RT   "The complete genome sequence of the lactic acid bacterium Lactococcus
RT   lactis ssp. lactis IL1403.";
RL   Genome Res. 11:731-753(2001).
CC   -!- FUNCTION: Involved in a multicomponent binding-protein-dependent
CC       transport system for glycine betaine. Probably responsible for energy
CC       coupling to the transport system.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O + quaternary ammonium(out) = ADP + H(+) + phosphate
CC         + quaternary ammonium(in); Xref=Rhea:RHEA:11036, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:35267,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=7.6.2.9;
CC   -!- SIMILARITY: Belongs to the ABC transporter superfamily. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF234619; AAF37878.1; -; Genomic_DNA.
DR   EMBL; AE005176; AAK05550.1; -; Genomic_DNA.
DR   PIR; D86806; D86806.
DR   RefSeq; NP_267608.1; NC_002662.1.
DR   RefSeq; WP_003130443.1; NC_002662.1.
DR   AlphaFoldDB; Q9KIF7; -.
DR   SMR; Q9KIF7; -.
DR   STRING; 272623.L74195; -.
DR   PaxDb; Q9KIF7; -.
DR   EnsemblBacteria; AAK05550; AAK05550; L74195.
DR   GeneID; 66442373; -.
DR   KEGG; lla:L74195; -.
DR   PATRIC; fig|272623.7.peg.1562; -.
DR   eggNOG; COG3448; Bacteria.
DR   eggNOG; COG4175; Bacteria.
DR   HOGENOM; CLU_000604_2_0_9; -.
DR   OMA; GQIFVVM; -.
DR   BRENDA; 7.6.2.9; 2903.
DR   Proteomes; UP000002196; Chromosome.
DR   GO; GO:0016020; C:membrane; IEA:InterPro.
DR   GO; GO:0015418; F:ABC-type quaternary ammonium compound transporting activity; IEA:UniProtKB-EC.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0006865; P:amino acid transport; IEA:UniProtKB-KW.
DR   GO; GO:0031460; P:glycine betaine transport; IEA:InterPro.
DR   Gene3D; 3.10.580.10; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR   InterPro; IPR017871; ABC_transporter-like_CS.
DR   InterPro; IPR000644; CBS_dom.
DR   InterPro; IPR046342; CBS_dom_sf.
DR   InterPro; IPR005892; Gly-betaine_transp_ATP-bd.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF00005; ABC_tran; 1.
DR   Pfam; PF00571; CBS; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   SUPFAM; SSF54631; SSF54631; 1.
DR   TIGRFAMs; TIGR01186; proV; 1.
DR   PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR   PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
DR   PROSITE; PS51371; CBS; 2.
PE   3: Inferred from homology;
KW   Amino-acid transport; ATP-binding; CBS domain; Nucleotide-binding;
KW   Reference proteome; Repeat; Translocase; Transport.
FT   CHAIN           1..408
FT                   /note="Glycine betaine transport ATP-binding protein OpuAA"
FT                   /id="PRO_0000092675"
FT   DOMAIN          5..265
FT                   /note="ABC transporter"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
FT   DOMAIN          280..336
FT                   /note="CBS 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00703"
FT   DOMAIN          340..397
FT                   /note="CBS 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00703"
FT   BINDING         61..68
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00434"
SQ   SEQUENCE   408 AA;  45751 MW;  EBBA8A87BE563B88 CRC64;
     MPVKIKIEHL TKIFGKRIKT ALTMVEKGEP KNEILKKTGA TVGVYDTNFE INEGEIFVIM
     GLSGSGKSTL LRLLNRLIEP TSGKIFIDNQ DVATLNKEDL LQVRRKTMSM VFQNFGLFPH
     RTILENTEYG LEVQNVPKEE RRKRAEKALD NANLLDFKDQ YPKQLSGGMQ QRVGLARALA
     NDPEILLMDE AFSALDPLIR REMQDELLEL QAKFQKTIIF VSHDLNEALR IGDRIAIMKD
     GKIMQIGTGE EILTNPANDY VKTFVEDVDR AKVITAENIM IPALTTNIDV DGPSVALKKM
     KTEEVSSLMA VDKKRQFRGV VTSEQAIAAR KNNQPLKDVM TTDVGTVSKE MLVRDILPII
     YDAPTPLAVV DDNGFLKGVL IRGSVLEALA DIPDEDEVEE IEKEEENK