OPUAC_BACSU
ID OPUAC_BACSU Reviewed; 293 AA.
AC P46922;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Glycine betaine-binding protein OpuAC;
DE Flags: Precursor;
GN Name=opuAC; OrderedLocusNames=BSU03000;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBUNIT, AND SUBCELLULAR
RP LOCATION.
RC STRAIN=168 / JH642;
RX PubMed=7622480; DOI=10.1074/jbc.270.28.16701;
RA Kempf B., Bremer E.;
RT "OpuA, an osmotically regulated binding protein-dependent transport system
RT for the osmoprotectant glycine betaine in Bacillus subtilis.";
RL J. Biol. Chem. 270:16701-16713(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=8969502; DOI=10.1099/13500872-142-11-3047;
RA Yamane K., Kumano M., Kurita K.;
RT "The 25 degrees-36 degrees region of the Bacillus subtilis chromosome:
RT determination of the sequence of a 146 kb segment and identification of 113
RT genes.";
RL Microbiology 142:3047-3056(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 274-293.
RC STRAIN=168 / JH642;
RA Kempf B., Bremer E.;
RL Submitted (MAY-1996) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION IN GLYCINE BETAINE TRANSPORT.
RC STRAIN=168 / JH642;
RX PubMed=8752321; DOI=10.1128/jb.178.17.5071-5079.1996;
RA Kappes R., Kempf B., Bremer E.;
RT "Three transport systems for the osmoprotectant glycine betaine operate in
RT Bacillus subtilis: characterization of OpuD.";
RL J. Bacteriol. 178:5071-5079(1996).
RN [6]
RP INTERACTION WITH FLOT, AND SUBCELLULAR LOCATION.
RC STRAIN=168;
RX PubMed=23651456; DOI=10.1111/mmi.12252;
RA Bach J.N., Bramkamp M.;
RT "Flotillins functionally organize the bacterial membrane.";
RL Mol. Microbiol. 88:1205-1217(2013).
CC -!- FUNCTION: Involved in a multicomponent binding-protein-dependent
CC transport system for glycine betaine. {ECO:0000269|PubMed:7622480,
CC ECO:0000269|PubMed:8752321}.
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (OpuAA),
CC two transmembrane proteins (OpuAB) and a solute-binding protein (OpuAC)
CC (PubMed:7622480). Interacts with FloT (PubMed:23651456).
CC {ECO:0000269|PubMed:23651456, ECO:0000269|PubMed:7622480}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|PROSITE-
CC ProRule:PRU00303, ECO:0000269|PubMed:23651456,
CC ECO:0000269|PubMed:7622480}; Lipid-anchor {ECO:0000255|PROSITE-
CC ProRule:PRU00303, ECO:0000269|PubMed:7622480}. Membrane raft
CC {ECO:0000269|PubMed:23651456}; Lipid-anchor. Note=Present in detergent-
CC resistant membrane (DRM) fractions that may be equivalent to eukaryotic
CC membrane rafts; these rafts include proteins involved in signaling,
CC molecule trafficking and protein secretion.
CC {ECO:0000269|PubMed:23651456}.
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DR EMBL; U17292; AAC43457.1; -; Genomic_DNA.
DR EMBL; D50453; BAA08934.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB12094.1; -; Genomic_DNA.
DR EMBL; U47860; AAC44169.1; -; Genomic_DNA.
DR PIR; I40537; I40537.
DR RefSeq; NP_388182.1; NC_000964.3.
DR RefSeq; WP_003246462.1; NZ_JNCM01000030.1.
DR PDB; 2B4L; X-ray; 2.00 A; A=26-293.
DR PDB; 2B4M; X-ray; 2.80 A; A/B=26-293.
DR PDB; 3CHG; X-ray; 2.80 A; A/B/C/D=26-293.
DR PDB; 5NXX; X-ray; 2.20 A; C/D=26-293.
DR PDBsum; 2B4L; -.
DR PDBsum; 2B4M; -.
DR PDBsum; 3CHG; -.
DR PDBsum; 5NXX; -.
DR AlphaFoldDB; P46922; -.
DR SMR; P46922; -.
DR STRING; 224308.BSU03000; -.
DR TCDB; 3.A.1.12.2; the atp-binding cassette (abc) superfamily.
DR jPOST; P46922; -.
DR PaxDb; P46922; -.
DR EnsemblBacteria; CAB12094; CAB12094; BSU_03000.
DR GeneID; 938354; -.
DR KEGG; bsu:BSU03000; -.
DR PATRIC; fig|224308.179.peg.312; -.
DR eggNOG; COG2113; Bacteria.
DR InParanoid; P46922; -.
DR OMA; NTHKAYA; -.
DR PhylomeDB; P46922; -.
DR BioCyc; BSUB:BSU03000-MON; -.
DR BRENDA; 7.6.2.9; 658.
DR EvolutionaryTrace; P46922; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0043190; C:ATP-binding cassette (ABC) transporter complex; IEA:InterPro.
DR GO; GO:0045121; C:membrane raft; IEA:UniProtKB-SubCell.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0006865; P:amino acid transport; IEA:UniProtKB-KW.
DR InterPro; IPR007210; ABC_Gly_betaine_transp_sub-bd.
DR Pfam; PF04069; OpuAC; 2.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Amino-acid transport; Cell membrane; Lipoprotein; Membrane;
KW Palmitate; Reference proteome; Signal; Transport.
FT SIGNAL 1..20
FT /evidence="ECO:0000305"
FT CHAIN 21..293
FT /note="Glycine betaine-binding protein OpuAC"
FT /id="PRO_0000031844"
FT LIPID 21
FT /note="N-palmitoyl cysteine"
FT /evidence="ECO:0000305"
FT LIPID 21
FT /note="S-diacylglycerol cysteine"
FT /evidence="ECO:0000305"
FT HELIX 31..34
FT /evidence="ECO:0007829|PDB:2B4L"
FT TURN 35..37
FT /evidence="ECO:0007829|PDB:2B4L"
FT STRAND 38..40
FT /evidence="ECO:0007829|PDB:2B4L"
FT HELIX 47..58
FT /evidence="ECO:0007829|PDB:2B4L"
FT STRAND 65..67
FT /evidence="ECO:0007829|PDB:2B4L"
FT HELIX 71..83
FT /evidence="ECO:0007829|PDB:2B4L"
FT STRAND 90..95
FT /evidence="ECO:0007829|PDB:2B4L"
FT HELIX 98..101
FT /evidence="ECO:0007829|PDB:2B4L"
FT STRAND 104..106
FT /evidence="ECO:0007829|PDB:2B4L"
FT STRAND 116..125
FT /evidence="ECO:0007829|PDB:2B4L"
FT HELIX 128..131
FT /evidence="ECO:0007829|PDB:2B4L"
FT HELIX 133..140
FT /evidence="ECO:0007829|PDB:2B4L"
FT HELIX 146..157
FT /evidence="ECO:0007829|PDB:2B4L"
FT HELIX 162..172
FT /evidence="ECO:0007829|PDB:2B4L"
FT HELIX 174..180
FT /evidence="ECO:0007829|PDB:2B4L"
FT TURN 181..183
FT /evidence="ECO:0007829|PDB:2B4L"
FT STRAND 191..196
FT /evidence="ECO:0007829|PDB:2B4L"
FT HELIX 200..216
FT /evidence="ECO:0007829|PDB:2B4L"
FT STRAND 219..224
FT /evidence="ECO:0007829|PDB:2B4L"
FT HELIX 229..235
FT /evidence="ECO:0007829|PDB:2B4L"
FT STRAND 238..247
FT /evidence="ECO:0007829|PDB:2B4L"
FT TURN 248..251
FT /evidence="ECO:0007829|PDB:2B4L"
FT HELIX 252..257
FT /evidence="ECO:0007829|PDB:2B4L"
FT TURN 258..260
FT /evidence="ECO:0007829|PDB:2B4L"
FT STRAND 261..279
FT /evidence="ECO:0007829|PDB:2B4L"
FT HELIX 288..291
FT /evidence="ECO:0007829|PDB:2B4L"
SQ SEQUENCE 293 AA; 32215 MW; B2BE74985069C4C7 CRC64;
MLKKIIGIGV SAMLALSLAA CGSENDENAS AAEQVNKTII GIDPGSGIMS LTDKAMKDYD
LNDWTLISAS SAAMTATLKK SYDRKKPIII TGWTPHWMFS RYKLKYLDDP KQSYGSAEEI
HTITRKGFSK EQPNAAKLLS QFKWTQDEMG EIMIKVEEGE KPAKVAAEYV NKHKDQIAEW
TKGVQKVKGD KINLAYVAWD SEIASTNVIG KVLEDLGYEV TLTQVEAGPM WTAIATGSAD
ASLSAWLPNT HKAYAAKYKG KYDDIGTSMT GVKMGLVVPQ YMKNVNSIED LKK
