OPUBC_BACSU
ID OPUBC_BACSU Reviewed; 306 AA.
AC Q45462; O34432;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2000, sequence version 2.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Choline-binding protein;
DE Flags: Precursor;
GN Name=opuBC; Synonyms=proX; OrderedLocusNames=BSU33710;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 6633 / PCI 219 / NRS 231;
RX PubMed=7592481; DOI=10.1128/jb.177.23.6874-6880.1995;
RA Lin Y., Hansen J.N.;
RT "Characterization of a chimeric proU operon in a subtilin-producing mutant
RT of Bacillus subtilis 168.";
RL J. Bacteriol. 177:6874-6880(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=168 / JH642;
RX PubMed=10216873; DOI=10.1046/j.1365-2958.1999.01354.x;
RA Kappes R.M., Kempf B., Kneip S., Boch J., Gade J., Meier-Wagner J.,
RA Bremer E.;
RT "Two evolutionarily closely related ABC transporters mediate the uptake of
RT choline for synthesis of the osmoprotectant glycine betaine in Bacillus
RT subtilis.";
RL Mol. Microbiol. 32:203-216(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [4]
RP INDUCTION.
RC STRAIN=168 / JH642;
RX PubMed=22408163; DOI=10.1128/jb.06642-11;
RA Nau-Wagner G., Opper D., Rolbetzki A., Boch J., Kempf B., Hoffmann T.,
RA Bremer E.;
RT "Genetic control of osmoadaptive glycine betaine synthesis in Bacillus
RT subtilis through the choline-sensing and glycine betaine-responsive GbsR
RT repressor.";
RL J. Bacteriol. 194:2703-2714(2012).
CC -!- FUNCTION: Member of a high affinity multicomponent binding-protein-
CC dependent transport system for choline. {ECO:0000269|PubMed:10216873}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor
CC {ECO:0000305}.
CC -!- INDUCTION: Repressed by GbsR. {ECO:0000269|PubMed:22408163}.
CC -!- SIMILARITY: Belongs to the OsmX family. {ECO:0000305}.
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DR EMBL; U38418; AAB01534.1; -; Genomic_DNA.
DR EMBL; AF008930; AAC14358.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB15376.1; -; Genomic_DNA.
DR PIR; A69670; A69670.
DR RefSeq; NP_391251.1; NC_000964.3.
DR RefSeq; WP_003228372.1; NZ_JNCM01000033.1.
DR PDB; 3R6U; X-ray; 1.61 A; A=23-306.
DR PDB; 5NXY; X-ray; 1.90 A; A/C=23-306.
DR PDB; 6EYG; X-ray; 1.42 A; A=1-306.
DR PDB; 6EYH; X-ray; 1.60 A; A=1-306.
DR PDB; 6EYL; X-ray; 1.50 A; A/B=1-306.
DR PDB; 6EYQ; X-ray; 1.50 A; A/B=1-306.
DR PDBsum; 3R6U; -.
DR PDBsum; 5NXY; -.
DR PDBsum; 6EYG; -.
DR PDBsum; 6EYH; -.
DR PDBsum; 6EYL; -.
DR PDBsum; 6EYQ; -.
DR AlphaFoldDB; Q45462; -.
DR SMR; Q45462; -.
DR STRING; 224308.BSU33710; -.
DR TCDB; 3.A.1.12.3; the atp-binding cassette (abc) superfamily.
DR PaxDb; Q45462; -.
DR PRIDE; Q45462; -.
DR EnsemblBacteria; CAB15376; CAB15376; BSU_33710.
DR GeneID; 938475; -.
DR KEGG; bsu:BSU33710; -.
DR PATRIC; fig|224308.179.peg.3656; -.
DR eggNOG; COG1732; Bacteria.
DR InParanoid; Q45462; -.
DR OMA; APMNNTY; -.
DR PhylomeDB; Q45462; -.
DR BioCyc; BSUB:BSU33710-MON; -.
DR BRENDA; 7.6.2.9; 658.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0043190; C:ATP-binding cassette (ABC) transporter complex; IEA:InterPro.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0006865; P:amino acid transport; IEA:UniProtKB-KW.
DR GO; GO:0031460; P:glycine betaine transport; IBA:GO_Central.
DR InterPro; IPR007210; ABC_Gly_betaine_transp_sub-bd.
DR Pfam; PF04069; OpuAC; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Amino-acid transport; Cell membrane; Lipoprotein; Membrane;
KW Palmitate; Reference proteome; Signal; Transport.
FT SIGNAL 1..22
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT CHAIN 23..306
FT /note="Choline-binding protein"
FT /id="PRO_0000031845"
FT LIPID 23
FT /note="N-palmitoyl cysteine"
FT /evidence="ECO:0000305"
FT LIPID 23
FT /note="S-diacylglycerol cysteine"
FT /evidence="ECO:0000305"
FT VARIANT 8
FT /note="L -> W (in strain: ATCC 6633)"
FT VARIANT 13
FT /note="A -> T (in strain: ATCC 6633)"
FT VARIANT 32
FT /note="A -> S (in strain: ATCC 6633)"
FT VARIANT 84
FT /note="G -> R (in strain: ATCC 6633)"
FT VARIANT 111
FT /note="D -> E (in strain: ATCC 6633)"
FT VARIANT 146
FT /note="E -> K (in strain: ATCC 6633)"
FT VARIANT 155
FT /note="T -> N (in strain: ATCC 6633)"
FT VARIANT 194
FT /note="G -> S (in strain: ATCC 6633)"
FT VARIANT 252
FT /note="K -> Q (in strain: ATCC 6633)"
FT CONFLICT 17..19
FT /note="TLT -> MLP (in Ref. 1; AAB01534)"
FT /evidence="ECO:0000305"
FT CONFLICT 172..188
FT /note="NYWMKLKGNGYQDFTKT -> KVLDEAQGERLSRFYEN (in Ref. 1;
FT AAB01534)"
FT /evidence="ECO:0000305"
FT CONFLICT 263..268
FT /note="IIKKML -> TIQKMI (in Ref. 1; AAB01534)"
FT /evidence="ECO:0000305"
FT CONFLICT 296..306
FT /note="EYLEKHRYFES -> AIFRKAPLLRIVKGGRSQ (in Ref. 1;
FT AAB01534)"
FT /evidence="ECO:0000305"
FT HELIX 28..33
FT /evidence="ECO:0007829|PDB:6EYG"
FT STRAND 36..43
FT /evidence="ECO:0007829|PDB:6EYG"
FT HELIX 44..60
FT /evidence="ECO:0007829|PDB:6EYG"
FT STRAND 65..71
FT /evidence="ECO:0007829|PDB:6EYG"
FT HELIX 74..82
FT /evidence="ECO:0007829|PDB:6EYG"
FT STRAND 87..93
FT /evidence="ECO:0007829|PDB:6EYG"
FT HELIX 94..100
FT /evidence="ECO:0007829|PDB:6EYG"
FT HELIX 110..125
FT /evidence="ECO:0007829|PDB:6EYG"
FT STRAND 127..129
FT /evidence="ECO:0007829|PDB:6EYG"
FT STRAND 139..143
FT /evidence="ECO:0007829|PDB:6EYG"
FT HELIX 145..151
FT /evidence="ECO:0007829|PDB:6EYG"
FT STRAND 154..156
FT /evidence="ECO:0007829|PDB:5NXY"
FT HELIX 157..165
FT /evidence="ECO:0007829|PDB:6EYG"
FT STRAND 167..170
FT /evidence="ECO:0007829|PDB:6EYG"
FT HELIX 174..176
FT /evidence="ECO:0007829|PDB:6EYG"
FT STRAND 178..181
FT /evidence="ECO:0007829|PDB:6EYG"
FT HELIX 182..189
FT /evidence="ECO:0007829|PDB:6EYG"
FT STRAND 194..198
FT /evidence="ECO:0007829|PDB:6EYG"
FT HELIX 201..203
FT /evidence="ECO:0007829|PDB:6EYG"
FT HELIX 204..209
FT /evidence="ECO:0007829|PDB:6EYG"
FT STRAND 212..219
FT /evidence="ECO:0007829|PDB:6EYG"
FT HELIX 224..227
FT /evidence="ECO:0007829|PDB:6EYG"
FT STRAND 230..232
FT /evidence="ECO:0007829|PDB:6EYL"
FT STRAND 244..250
FT /evidence="ECO:0007829|PDB:6EYG"
FT HELIX 251..256
FT /evidence="ECO:0007829|PDB:6EYG"
FT HELIX 260..264
FT /evidence="ECO:0007829|PDB:6EYG"
FT HELIX 265..267
FT /evidence="ECO:0007829|PDB:6EYG"
FT HELIX 273..284
FT /evidence="ECO:0007829|PDB:6EYG"
FT HELIX 290..300
FT /evidence="ECO:0007829|PDB:6EYG"
FT HELIX 302..304
FT /evidence="ECO:0007829|PDB:6EYG"
SQ SEQUENCE 306 AA; 34401 MW; 9D8B32E1F5E7AD0F CRC64;
MKRKYLKLMI GLALAATLTL SGCSLPGLSA AADQTIKIGA QSMSESEIIA SMLGQLIEHH
TDLKTTTIKN LGSNAVQQQA LMNGEIDIAA TRYTGDALTG TLRMEPEKDP DKALALTQRE
FKKRYDLKWY DSYGFDNTYA FTVSKELADQ YHLETVSDVK KWAPQLKLGV DNYWMKLKGN
GYQDFTKTYG MTFGGTYPMQ IGLVYDAVKS GKMDIVLAYS TDGRIKSYGL KMLKDDKQFF
PPYDCSPVVP EKVLKEHPEL EGIIKKMLGK IDTATMQELN YEVDGNLKEP SVVAKEYLEK
HRYFES
