OPUBD_BACSU
ID OPUBD_BACSU Reviewed; 226 AA.
AC P39775; O34657;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 30-MAY-2000, sequence version 3.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Choline transport system permease protein OpuBD;
GN Name=opuBD; Synonyms=proZ; OrderedLocusNames=BSU33700;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 6633 / PCI 219 / NRS 231;
RX PubMed=7592481; DOI=10.1128/jb.177.23.6874-6880.1995;
RA Lin Y., Hansen J.N.;
RT "Characterization of a chimeric proU operon in a subtilin-producing mutant
RT of Bacillus subtilis 168.";
RL J. Bacteriol. 177:6874-6880(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=168 / JH642;
RX PubMed=10216873; DOI=10.1046/j.1365-2958.1999.01354.x;
RA Kappes R.M., Kempf B., Kneip S., Boch J., Gade J., Meier-Wagner J.,
RA Bremer E.;
RT "Two evolutionarily closely related ABC transporters mediate the uptake of
RT choline for synthesis of the osmoprotectant glycine betaine in Bacillus
RT subtilis.";
RL Mol. Microbiol. 32:203-216(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 21-226.
RC STRAIN=ATCC 6633 / PCI 219 / NRS 231;
RX PubMed=1400221; DOI=10.1128/jb.174.20.6699-6702.1992;
RA Chung Y.J., Hansen J.N.;
RT "Determination of the sequence of spaE and identification of a promoter in
RT the subtilin (spa) operon in Bacillus subtilis.";
RL J. Bacteriol. 174:6699-6702(1992).
RN [5]
RP INDUCTION.
RC STRAIN=168 / JH642;
RX PubMed=22408163; DOI=10.1128/jb.06642-11;
RA Nau-Wagner G., Opper D., Rolbetzki A., Boch J., Kempf B., Hoffmann T.,
RA Bremer E.;
RT "Genetic control of osmoadaptive glycine betaine synthesis in Bacillus
RT subtilis through the choline-sensing and glycine betaine-responsive GbsR
RT repressor.";
RL J. Bacteriol. 194:2703-2714(2012).
CC -!- FUNCTION: Involved in a high affinity multicomponent binding-protein-
CC dependent transport system for choline; probably responsible for the
CC translocation of the substrate across the membrane.
CC {ECO:0000269|PubMed:10216873}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000255|PROSITE-ProRule:PRU00441}.
CC -!- INDUCTION: Repressed by GbsR. {ECO:0000269|PubMed:22408163}.
CC -!- SIMILARITY: Belongs to the binding-protein-dependent transport system
CC permease family. CysTW subfamily. {ECO:0000305}.
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DR EMBL; U38418; AAB01535.1; -; Genomic_DNA.
DR EMBL; AF008930; AAC14359.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB15375.1; -; Genomic_DNA.
DR EMBL; M99263; AAA22773.1; -; Genomic_DNA.
DR PIR; B69670; B69670.
DR RefSeq; NP_391250.1; NC_000964.3.
DR RefSeq; WP_003228374.1; NZ_JNCM01000033.1.
DR AlphaFoldDB; P39775; -.
DR SMR; P39775; -.
DR STRING; 224308.BSU33700; -.
DR TCDB; 3.A.1.12.3; the atp-binding cassette (abc) superfamily.
DR PaxDb; P39775; -.
DR PRIDE; P39775; -.
DR EnsemblBacteria; CAB15375; CAB15375; BSU_33700.
DR GeneID; 936226; -.
DR KEGG; bsu:BSU33700; -.
DR PATRIC; fig|224308.179.peg.3655; -.
DR eggNOG; COG1174; Bacteria.
DR InParanoid; P39775; -.
DR OMA; CLARNEW; -.
DR PhylomeDB; P39775; -.
DR BioCyc; BSUB:BSU33700-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0006865; P:amino acid transport; IEA:UniProtKB-KW.
DR GO; GO:0031460; P:glycine betaine transport; IBA:GO_Central.
DR CDD; cd06261; TM_PBP2; 1.
DR Gene3D; 1.10.3720.10; -; 1.
DR InterPro; IPR000515; MetI-like.
DR InterPro; IPR035906; MetI-like_sf.
DR Pfam; PF00528; BPD_transp_1; 1.
DR SUPFAM; SSF161098; SSF161098; 1.
DR PROSITE; PS50928; ABC_TM1; 1.
PE 2: Evidence at transcript level;
KW Amino-acid transport; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..226
FT /note="Choline transport system permease protein OpuBD"
FT /id="PRO_0000060155"
FT TRANSMEM 27..47
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 52..72
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 73..93
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 148..168
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT TRANSMEM 182..202
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT DOMAIN 22..202
FT /note="ABC transmembrane type-1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT VARIANT 30
FT /note="A -> V (in strain: ATCC 6633 / PCI 219)"
FT VARIANT 43
FT /note="A -> V (in strain: ATCC 6633 / PCI 219)"
FT VARIANT 50
FT /note="F -> Y (in strain: ATCC 6633 / PCI 219)"
FT VARIANT 89
FT /note="L -> I (in strain: ATCC 6633 / PCI 219)"
FT VARIANT 108
FT /note="I -> V (in strain: ATCC 6633 / PCI 219)"
FT VARIANT 189
FT /note="I -> T (in strain: ATCC 6633 / PCI 219)"
FT VARIANT 196
FT /note="V -> I (in strain: ATCC 6633 / PCI 219)"
FT VARIANT 201
FT /note="L -> I (in strain: ATCC 6633 / PCI 219)"
FT VARIANT 205
FT /note="L -> I (in strain: ATCC 6633 / PCI 219)"
FT CONFLICT 208..210
FT /note="ALS -> FLN (in Ref. 1 and 4)"
FT /evidence="ECO:0000305"
FT CONFLICT 214..226
FT /note="KKRTGAKHVQSAA -> QKSRRKVISV (in Ref. 1 and 4)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 226 AA; 23928 MW; DA6D47BAFF8347E9 CRC64;
MNVLEQLMTY YAQNGSYVMD EFGRHFLMSA YGVLFAAVVG VPAGILIAHF RRLSAWVFAV
TNVIQTIPAL AMLAVLMLVM GLGANTVILS LFLYSLLPII RNTYTGIISI EHAYLESGKA
MGMTKFQVLR MVELPLALSV IMAGLRTALV IAIGITAIGT FVGAGGLGDM IVRGSNATNG
TAIILAGAIP TAVMAVGADL LMAWLERALS PVKKKRTGAK HVQSAA
