OPUD_BACSU
ID OPUD_BACSU Reviewed; 512 AA.
AC P54417;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Glycine betaine transporter OpuD;
GN Name=opuD; Synonyms=ytfQ; OrderedLocusNames=BSU30070;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN GLYCINE BETAINE TRANSPORT,
RP ACTIVITY REGULATION, INDUCTION, AND GENE NAME.
RC STRAIN=168 / JH642;
RX PubMed=8752321; DOI=10.1128/jb.178.17.5071-5079.1996;
RA Kappes R., Kempf B., Bremer E.;
RT "Three transport systems for the osmoprotectant glycine betaine operate in
RT Bacillus subtilis: characterization of OpuD.";
RL J. Bacteriol. 178:5071-5079(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9387221; DOI=10.1099/00221287-143-11-3431;
RA Lapidus A., Galleron N., Sorokin A., Ehrlich S.D.;
RT "Sequencing and functional annotation of the Bacillus subtilis genes in the
RT 200 kb rrnB-dnaB region.";
RL Microbiology 143:3431-3441(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
CC -!- FUNCTION: High-affinity uptake of glycine betaine. Does not mediate
CC either carnitine or choline uptake. {ECO:0000269|PubMed:8752321}.
CC -!- ACTIVITY REGULATION: Activity is stimulated by high osmolarity.
CC {ECO:0000305|PubMed:8752321}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- INDUCTION: Induced by high osmolarity. {ECO:0000305|PubMed:8752321}.
CC -!- SIMILARITY: Belongs to the BCCT transporter (TC 2.A.15) family.
CC {ECO:0000305}.
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DR EMBL; U50082; AAC44368.1; -; Genomic_DNA.
DR EMBL; AF008220; AAC00408.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB14985.1; -; Genomic_DNA.
DR PIR; G69670; G69670.
DR RefSeq; NP_390885.1; NC_000964.3.
DR RefSeq; WP_003229220.1; NZ_JNCM01000036.1.
DR AlphaFoldDB; P54417; -.
DR SMR; P54417; -.
DR STRING; 224308.BSU30070; -.
DR TCDB; 2.A.15.1.1; the betaine/carnitine/choline transporter (bcct) family.
DR PaxDb; P54417; -.
DR PRIDE; P54417; -.
DR EnsemblBacteria; CAB14985; CAB14985; BSU_30070.
DR GeneID; 937986; -.
DR KEGG; bsu:BSU30070; -.
DR PATRIC; fig|224308.179.peg.3264; -.
DR eggNOG; COG1292; Bacteria.
DR InParanoid; P54417; -.
DR OMA; VHAWAIY; -.
DR PhylomeDB; P54417; -.
DR BioCyc; BSUB:BSU30070-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0031460; P:glycine betaine transport; IMP:CACAO.
DR InterPro; IPR018093; BCCT_CS.
DR InterPro; IPR000060; BCCT_transptr.
DR PANTHER; PTHR30047; PTHR30047; 1.
DR Pfam; PF02028; BCCT; 1.
DR TIGRFAMs; TIGR00842; bcct; 1.
DR PROSITE; PS01303; BCCT; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..512
FT /note="Glycine betaine transporter OpuD"
FT /id="PRO_0000201493"
FT TRANSMEM 5..25
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 45..65
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 82..102
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 135..155
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 186..206
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 222..242
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 257..277
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 312..332
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 343..363
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 395..415
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 441..461
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 464..484
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 512 AA; 56122 MW; F1D8B1EEBE30F1FF CRC64;
MLKHISSVFW IVIAITAAAV LWGVISPDSL QNVSQSAQAF ITDSFGWYYL LVVSLFVGFC
LFLIFSPIGK IKLGKPDEKP EFGLLSWFAM LFSAGMGIGL VFYGAAEPIS HYAISSPSGE
TETPQAFRDA LRYTFFHWGL HAWAIYAIVA LCIAYFQFRK GAPGLISSTL SPILGDKVNG
PIGKAIDCIA VFATVVGVST SLGLGATQIN GGLNYLFGIP NAFIVQLVLI IIVTVLFLLS
AWSGLGKGIK YLSNTNMVLA GLLMLFMLVV GPTVLIMNSF TDSIGQYIQN IVQMSFRLTP
NDPEKREWIN SWTIFYWAWW ISWSPFVGIF IARVSRGRTI REFLIGVLVT PCILTFLWFS
IFGVSAMDLQ QKGAFNVAKL STETMLFGTL DHYPLTMVTS ILALILIAVF FITSADSATF
VLGMQTSYGS LNPANSVKLS WGIIQSAMAA VLLYSGGLAA LQNTAILAAL PFSIVILLMI
ASLYQSLSKE RREIKKAEKL DKPRSPRVKK AY
