OR2A_DROME
ID OR2A_DROME Reviewed; 397 AA.
AC O46077; Q9W504;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2000, sequence version 2.
DT 03-AUG-2022, entry version 161.
DE RecName: Full=Odorant receptor 2a;
GN Name=Or2a; Synonyms=AN4, DOR2F.1, dor62, OR2F.1; ORFNames=CG3206;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=10458908; DOI=10.1006/geno.1999.5894;
RA Gao Q., Chess A.;
RT "Identification of candidate Drosophila olfactory receptors from genomic
RT DNA sequence.";
RL Genomics 60:31-39(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Oregon-R;
RX PubMed=10731137; DOI=10.1126/science.287.5461.2220;
RA Benos P.V., Gatt M.K., Ashburner M., Murphy L., Harris D., Barrell B.G.,
RA Ferraz C., Vidal S., Brun C., Demailles J., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Borkova D., Minana B., Kafatos F.C.,
RA Louis C., Siden-Kiamos I., Bolshakov S., Papagiannakis G., Spanos L.,
RA Cox S., Madueno E., de Pablos B., Modolell J., Peter A., Schoettler P.,
RA Werner M., Mourkioti F., Beinert N., Dowe G., Schaefer U., Jaeckle H.,
RA Bucheton A., Callister D.M., Campbell L.A., Darlamitsou A., Henderson N.S.,
RA McMillan P.J., Salles C., Tait E.A., Valenti P., Saunders R.D.C.,
RA Glover D.M.;
RT "From sequence to chromosome: the tip of the X chromosome of D.
RT melanogaster.";
RL Science 287:2220-2222(2000).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 245-397, AND TISSUE SPECIFICITY.
RC STRAIN=Oregon-R; TISSUE=Antenna;
RX PubMed=10089887; DOI=10.1016/s0092-8674(00)80582-6;
RA Vosshall L.B., Amrein H., Morozov P.S., Rzhetsky A., Axel R.;
RT "A spatial map of olfactory receptor expression in the Drosophila
RT antenna.";
RL Cell 96:725-736(1999).
RN [6]
RP IDENTIFICATION, AND TISSUE SPECIFICITY.
RX PubMed=10069338; DOI=10.1016/s0896-6273(00)81093-4;
RA Clyne P.J., Warr C.G., Freeman M.R., Lessing D., Kim J., Carlson J.R.;
RT "A novel family of divergent seven-transmembrane proteins: candidate
RT odorant receptors in Drosophila.";
RL Neuron 22:327-338(1999).
RN [7]
RP TISSUE SPECIFICITY.
RX PubMed=10943836; DOI=10.1016/s0092-8674(00)00021-0;
RA Vosshall L.B., Wong A.M., Axel R.;
RT "An olfactory sensory map in the fly brain.";
RL Cell 102:147-159(2000).
RN [8]
RP FUNCTION.
RX PubMed=16615896; DOI=10.1016/j.cell.2006.01.050;
RA Hallem E.A., Carlson J.R.;
RT "Coding of odors by a receptor repertoire.";
RL Cell 125:143-160(2006).
RN [9]
RP FUNCTION.
RX PubMed=21613503; DOI=10.1523/jneurosci.6254-10.2011;
RA Montague S.A., Mathew D., Carlson J.R.;
RT "Similar odorants elicit different behavioral and physiological responses,
RT some supersustained.";
RL J. Neurosci. 31:7891-7899(2011).
CC -!- FUNCTION: Odorant receptor which mediates acceptance or avoidance
CC behavior, depending on its substrates. The odorant receptor repertoire
CC encodes a large collection of odor stimuli that vary widely in
CC identity, intensity, and duration. May form a complex with Orco to form
CC odorant-sensing units, providing sensitive and prolonged odorant
CC signaling and calcium permeability. {ECO:0000269|PubMed:16615896,
CC ECO:0000269|PubMed:21613503}.
CC -!- SUBUNIT: Interacts with Orco. Complexes exist early in the endomembrane
CC system in olfactory sensory neurons (OSNs), coupling these complexes to
CC the conserved ciliary trafficking pathway (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in 20 sensory neurons on the distal edge
CC of the antenna. {ECO:0000269|PubMed:10069338,
CC ECO:0000269|PubMed:10089887, ECO:0000269|PubMed:10943836}.
CC -!- MISCELLANEOUS: The atypical heteromeric and topological design of the
CC odorant receptors appears to be an insect-specific solution for odor
CC recognition, making the OR/Orco complex an attractive target for the
CC development of highly selective insect repellents to disrupt olfactory-
CC mediated host-seeking behaviors of insect disease vectors. Odor-evoked
CC OR currents are independent of known G-protein-coupled second messenger
CC pathways.
CC -!- SIMILARITY: Belongs to the insect chemoreceptor superfamily.
CC Heteromeric odorant receptor channel (TC 1.A.69) family. Or2a
CC subfamily. {ECO:0000305}.
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DR EMBL; AE014298; AAF45759.1; -; Genomic_DNA.
DR EMBL; AL009195; CAA15703.1; -; Genomic_DNA.
DR EMBL; AF127921; AAD26356.1; -; mRNA.
DR PIR; T13426; T13426.
DR RefSeq; NP_525046.1; NM_080307.2.
DR AlphaFoldDB; O46077; -.
DR SMR; O46077; -.
DR BioGRID; 57744; 1.
DR DIP; DIP-23378N; -.
DR STRING; 7227.FBpp0070381; -.
DR PaxDb; O46077; -.
DR EnsemblMetazoa; FBtr0070397; FBpp0070381; FBgn0023523.
DR GeneID; 31207; -.
DR KEGG; dme:Dmel_CG3206; -.
DR CTD; 31207; -.
DR FlyBase; FBgn0023523; Or2a.
DR VEuPathDB; VectorBase:FBgn0023523; -.
DR eggNOG; ENOG502SAW0; Eukaryota.
DR GeneTree; ENSGT00540000073151; -.
DR HOGENOM; CLU_033399_8_1_1; -.
DR InParanoid; O46077; -.
DR OMA; CYFGDRM; -.
DR OrthoDB; 745330at2759; -.
DR PhylomeDB; O46077; -.
DR BioGRID-ORCS; 31207; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 31207; -.
DR PRO; PR:O46077; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0023523; Expressed in sensory neuron and 2 other tissues.
DR ExpressionAtlas; O46077; baseline and differential.
DR Genevisible; O46077; DM.
DR GO; GO:0032590; C:dendrite membrane; ISS:FlyBase.
DR GO; GO:0016021; C:integral component of membrane; HDA:FlyBase.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005549; F:odorant binding; ISS:FlyBase.
DR GO; GO:0004984; F:olfactory receptor activity; HDA:FlyBase.
DR GO; GO:0004888; F:transmembrane signaling receptor activity; ISM:FlyBase.
DR GO; GO:0050911; P:detection of chemical stimulus involved in sensory perception of smell; HDA:FlyBase.
DR GO; GO:0007608; P:sensory perception of smell; IEP:FlyBase.
DR GO; GO:0007165; P:signal transduction; IEA:UniProtKB-KW.
DR InterPro; IPR004117; 7tm6_olfct_rcpt.
DR PANTHER; PTHR21137; PTHR21137; 1.
DR Pfam; PF02949; 7tm_6; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Membrane; Olfaction; Receptor; Reference proteome;
KW Sensory transduction; Transducer; Transmembrane; Transmembrane helix.
FT CHAIN 1..397
FT /note="Odorant receptor 2a"
FT /id="PRO_0000174225"
FT TOPO_DOM 1..38
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 39..59
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 60..72
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 73..92
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 93..131
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 132..150
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 151..176
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 177..197
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 198..272
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 273..293
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 294..301
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 302..322
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 323..363
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 364..383
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 384..397
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT CONFLICT 382..397
FT /note="VMRFTYSVFTLLLRAK -> ARHNYIFKCPLI (in Ref. 1 and 4;
FT CAA15703)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 397 AA; 45935 MW; 624C9783627FF2A3 CRC64;
MEKQEDFKLN THSAVYYHWR VWELTGLMRP PGVSSLLYVV YSITVNLVVT VLFPLSLLAR
LLFTTNMAGL CENLTITITD IVANLKFANV YMVRKQLHEI RSLLRLMDAR ARLVGDPEEI
SALRKEVNIA QGTFRTFASI FVFGTTLSCV RVVVRPDREL LYPAWFGVDW MHSTRNYVLI
NIYQLFGLIV QAIQNCASDS YPPAFLCLLT GHMRALELRV RRIGCRTEKS NKGQTYEAWR
EEVYQELIEC IRDLARVHRL REIIQRVLSV PCMAQFVCSA AVQCTVAMHF LYVADDHDHT
AMIISIVFFS AVTLEVFVIC YFGDRMRTQS EALCDAFYDC NWIEQLPKFK RELLFTLART
QRPSLIYAGN YIALSLETFE QVMRFTYSVF TLLLRAK
