OR33B_DROME
ID OR33B_DROME Reviewed; 379 AA.
AC P81915;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1999, sequence version 1.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Odorant receptor 33b;
GN Name=Or33b; Synonyms=AN1, DOR33B.2, dor72, Or33B.2; ORFNames=CG16961;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=10458908; DOI=10.1006/geno.1999.5894;
RA Gao Q., Chess A.;
RT "Identification of candidate Drosophila olfactory receptors from genomic
RT DNA sequence.";
RL Genomics 60:31-39(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP IDENTIFICATION, AND TISSUE SPECIFICITY.
RX PubMed=10069338; DOI=10.1016/s0896-6273(00)81093-4;
RA Clyne P.J., Warr C.G., Freeman M.R., Lessing D., Kim J., Carlson J.R.;
RT "A novel family of divergent seven-transmembrane proteins: candidate
RT odorant receptors in Drosophila.";
RL Neuron 22:327-338(1999).
RN [5]
RP IDENTIFICATION, AND TISSUE SPECIFICITY.
RC STRAIN=Oregon-R; TISSUE=Antenna;
RX PubMed=10089887; DOI=10.1016/s0092-8674(00)80582-6;
RA Vosshall L.B., Amrein H., Morozov P.S., Rzhetsky A., Axel R.;
RT "A spatial map of olfactory receptor expression in the Drosophila
RT antenna.";
RL Cell 96:725-736(1999).
RN [6]
RP TISSUE SPECIFICITY.
RX PubMed=10943836; DOI=10.1016/s0092-8674(00)00021-0;
RA Vosshall L.B., Wong A.M., Axel R.;
RT "An olfactory sensory map in the fly brain.";
RL Cell 102:147-159(2000).
RN [7]
RP FUNCTION.
RX PubMed=16615896; DOI=10.1016/j.cell.2006.01.050;
RA Hallem E.A., Carlson J.R.;
RT "Coding of odors by a receptor repertoire.";
RL Cell 125:143-160(2006).
RN [8]
RP FUNCTION.
RX PubMed=20577637; DOI=10.3389/fnbeh.2010.00027;
RA Bellmann D., Richardt A., Freyberger R., Nuwal N., Schwarzel M., Fiala A.,
RA Stortkuhl K.F.;
RT "Optogenetically induced olfactory stimulation in Drosophila larvae reveals
RT the neuronal basis of odor-aversion behavior.";
RL Front. Behav. Neurosci. 4:27-27(2010).
RN [9]
RP FUNCTION.
RX PubMed=21613503; DOI=10.1523/jneurosci.6254-10.2011;
RA Montague S.A., Mathew D., Carlson J.R.;
RT "Similar odorants elicit different behavioral and physiological responses,
RT some supersustained.";
RL J. Neurosci. 31:7891-7899(2011).
RN [10]
RP FUNCTION.
RX PubMed=21857978; DOI=10.1371/journal.pone.0022996;
RA Hoare D.J., Humble J., Jin D., Gilding N., Petersen R., Cobb M.,
RA McCrohan C.;
RT "Modeling peripheral olfactory coding in Drosophila larvae.";
RL PLoS ONE 6:E22996-E22996(2011).
CC -!- FUNCTION: Odorant receptor which mediates acceptance or avoidance
CC behavior, depending on its substrates. The odorant receptor repertoire
CC encodes a large collection of odor stimuli that vary widely in
CC identity, intensity, and duration. May form a complex with Orco to form
CC odorant-sensing units, providing sensitive and prolonged odorant
CC signaling and calcium permeability. Involved in the behavioral
CC responses to pentyl acetate and pyrazines.
CC {ECO:0000269|PubMed:16615896, ECO:0000269|PubMed:20577637,
CC ECO:0000269|PubMed:21613503, ECO:0000269|PubMed:21857978}.
CC -!- SUBUNIT: Interacts with Orco. Complexes exist early in the endomembrane
CC system in olfactory sensory neurons (OSNs), coupling these complexes to
CC the conserved ciliary trafficking pathway (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in 15 cells in the antenna but not the
CC maxillary palp. {ECO:0000269|PubMed:10069338,
CC ECO:0000269|PubMed:10089887, ECO:0000269|PubMed:10943836}.
CC -!- MISCELLANEOUS: The atypical heteromeric and topological design of the
CC odorant receptors appears to be an insect-specific solution for odor
CC recognition, making the OR/Orco complex an attractive target for the
CC development of highly selective insect repellents to disrupt olfactory-
CC mediated host-seeking behaviors of insect disease vectors. Odor-evoked
CC OR currents are independent of known G-protein-coupled second messenger
CC pathways.
CC -!- SIMILARITY: Belongs to the insect chemoreceptor superfamily.
CC Heteromeric odorant receptor channel (TC 1.A.69) family. Or2a
CC subfamily. {ECO:0000305}.
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DR EMBL; AE014134; AAF53132.1; -; Genomic_DNA.
DR RefSeq; NP_523554.1; NM_078830.1.
DR AlphaFoldDB; P81915; -.
DR SMR; P81915; -.
DR STRING; 7227.FBpp0079863; -.
DR PaxDb; P81915; -.
DR EnsemblMetazoa; FBtr0080279; FBpp0079863; FBgn0026391.
DR GeneID; 34602; -.
DR KEGG; dme:Dmel_CG16961; -.
DR CTD; 34602; -.
DR FlyBase; FBgn0026391; Or33b.
DR VEuPathDB; VectorBase:FBgn0026391; -.
DR eggNOG; ENOG502TBPZ; Eukaryota.
DR GeneTree; ENSGT00540000073151; -.
DR HOGENOM; CLU_033399_8_1_1; -.
DR InParanoid; P81915; -.
DR OMA; WHLLGLE; -.
DR OrthoDB; 745330at2759; -.
DR PhylomeDB; P81915; -.
DR BioGRID-ORCS; 34602; 0 hits in 1 CRISPR screen.
DR ChiTaRS; Or33b; fly.
DR GenomeRNAi; 34602; -.
DR PRO; PR:P81915; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0026391; Expressed in adult antennal lobe (Drosophila) and 5 other tissues.
DR Genevisible; P81915; DM.
DR GO; GO:0032590; C:dendrite membrane; ISS:FlyBase.
DR GO; GO:0016021; C:integral component of membrane; HDA:FlyBase.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005549; F:odorant binding; ISS:FlyBase.
DR GO; GO:0004984; F:olfactory receptor activity; HDA:FlyBase.
DR GO; GO:0004888; F:transmembrane signaling receptor activity; ISM:FlyBase.
DR GO; GO:0050911; P:detection of chemical stimulus involved in sensory perception of smell; HDA:FlyBase.
DR GO; GO:0007608; P:sensory perception of smell; IEP:FlyBase.
DR GO; GO:0007165; P:signal transduction; IEA:UniProtKB-KW.
DR InterPro; IPR004117; 7tm6_olfct_rcpt.
DR PANTHER; PTHR21137; PTHR21137; 1.
DR Pfam; PF02949; 7tm_6; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Membrane; Olfaction; Receptor; Reference proteome;
KW Sensory transduction; Transducer; Transmembrane; Transmembrane helix.
FT CHAIN 1..379
FT /note="Odorant receptor 33b"
FT /id="PRO_0000174239"
FT TOPO_DOM 1..37
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 38..58
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 59..64
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 65..85
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 86..129
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 130..150
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 151..165
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 166..186
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 187..256
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 257..277
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 278..281
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 282..302
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 303..355
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 356..376
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 377..379
FT /note="Extracellular"
FT /evidence="ECO:0000255"
SQ SEQUENCE 379 AA; 43633 MW; 4A50047EC1113C0E CRC64;
MDLKPRVIRS EDIYRTYWLY WHLLGLESNF FLNRLLDLVI TIFVTIWYPI HLILGLFMER
SLGDVCKGLP ITAACFFASF KFICFRFKLS EIKEIEILFK ELDQRALSRE ECEFFNQNTR
REANFIWKSF IVAYGLSNIS AIASVLFGGG HKLLYPAWFP YDVQATELIF WLSVTYQIAG
VSLAILQNLA NDSYPPMTFC VVAGHVRLLA MRLSRIGQGP EETIYLTGKQ LIESIEDHRK
LMKIVELLRS TMNISQLGQF ISSGVNISIT LVNILFFADN NFAITYYGVY FLSMVLELFP
CCYYGTLISV EMNQLTYAIY SSNWMSMNRS YSRILLIFMQ LTLAEVQIKA GGMIGIGMNA
FFATVRLAYS FFTLAMSLR
