OR42A_DROME
ID OR42A_DROME Reviewed; 406 AA.
AC Q9V9I2; Q29QF3;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 02-AUG-2002, sequence version 3.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=Odorant receptor 42a;
GN Name=Or42a; ORFNames=CG17250;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Celniker S.E.;
RL Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION.
RX PubMed=21613503; DOI=10.1523/jneurosci.6254-10.2011;
RA Montague S.A., Mathew D., Carlson J.R.;
RT "Similar odorants elicit different behavioral and physiological responses,
RT some supersustained.";
RL J. Neurosci. 31:7891-7899(2011).
RN [5]
RP FUNCTION.
RX PubMed=21857978; DOI=10.1371/journal.pone.0022996;
RA Hoare D.J., Humble J., Jin D., Gilding N., Petersen R., Cobb M.,
RA McCrohan C.;
RT "Modeling peripheral olfactory coding in Drosophila larvae.";
RL PLoS ONE 6:E22996-E22996(2011).
CC -!- FUNCTION: Odorant receptor which mediates acceptance or avoidance
CC behavior, depending on its substrates. The odorant receptor repertoire
CC encodes a large collection of odor stimuli that vary widely in
CC identity, intensity, and duration. May form a complex with Orco to form
CC odorant-sensing units, providing sensitive and prolonged odorant
CC signaling and calcium permeability. Involved in the behavioral
CC responses to butanol, ethyl acetate, propyl acetate, and pentyl
CC acetate. Responds also to pyrazines. {ECO:0000269|PubMed:21613503,
CC ECO:0000269|PubMed:21857978}.
CC -!- SUBUNIT: Interacts with Orco. Complexes exist early in the endomembrane
CC system in olfactory sensory neurons (OSNs), coupling these complexes to
CC the conserved ciliary trafficking pathway (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- MISCELLANEOUS: The atypical heteromeric and topological design of the
CC odorant receptors appears to be an insect-specific solution for odor
CC recognition, making the OR/Orco complex an attractive target for the
CC development of highly selective insect repellents to disrupt olfactory-
CC mediated host-seeking behaviors of insect disease vectors. Odor-evoked
CC OR currents are independent of known G-protein-coupled second messenger
CC pathways.
CC -!- SIMILARITY: Belongs to the insect chemoreceptor superfamily.
CC Heteromeric odorant receptor channel (TC 1.A.69) family. Or2a
CC subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABC86499.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AE013599; AAF57307.2; -; Genomic_DNA.
DR EMBL; BT024437; ABC86499.1; ALT_INIT; mRNA.
DR RefSeq; NP_523622.2; NM_078898.3.
DR AlphaFoldDB; Q9V9I2; -.
DR SMR; Q9V9I2; -.
DR BioGRID; 61420; 2.
DR DIP; DIP-22609N; -.
DR IntAct; Q9V9I2; 1.
DR STRING; 7227.FBpp0085388; -.
DR GlyGen; Q9V9I2; 1 site.
DR PaxDb; Q9V9I2; -.
DR DNASU; 35514; -.
DR EnsemblMetazoa; FBtr0086052; FBpp0085388; FBgn0033041.
DR GeneID; 35514; -.
DR KEGG; dme:Dmel_CG17250; -.
DR CTD; 35514; -.
DR FlyBase; FBgn0033041; Or42a.
DR VEuPathDB; VectorBase:FBgn0033041; -.
DR eggNOG; ENOG502TAX9; Eukaryota.
DR GeneTree; ENSGT00540000073151; -.
DR HOGENOM; CLU_033399_8_0_1; -.
DR InParanoid; Q9V9I2; -.
DR OMA; IFFFFMA; -.
DR OrthoDB; 694472at2759; -.
DR PhylomeDB; Q9V9I2; -.
DR BioGRID-ORCS; 35514; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 35514; -.
DR PRO; PR:Q9V9I2; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0033041; Expressed in mouthpart and 3 other tissues.
DR Genevisible; Q9V9I2; DM.
DR GO; GO:0032590; C:dendrite membrane; ISS:FlyBase.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005549; F:odorant binding; ISS:FlyBase.
DR GO; GO:0004984; F:olfactory receptor activity; IBA:GO_Central.
DR GO; GO:0050911; P:detection of chemical stimulus involved in sensory perception of smell; IMP:FlyBase.
DR GO; GO:0042048; P:olfactory behavior; IMP:FlyBase.
DR GO; GO:0007165; P:signal transduction; IEA:UniProtKB-KW.
DR InterPro; IPR004117; 7tm6_olfct_rcpt.
DR PANTHER; PTHR21137; PTHR21137; 1.
DR Pfam; PF02949; 7tm_6; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Glycoprotein; Membrane; Olfaction; Receptor;
KW Reference proteome; Sensory transduction; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..406
FT /note="Odorant receptor 42a"
FT /id="PRO_0000174242"
FT TOPO_DOM 1..44
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 45..65
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 66..86
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 87..107
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 108..142
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 143..163
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 164..181
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 182..202
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 203..271
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 272..292
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 293..298
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 299..319
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 320..359
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 360..380
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 381..406
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT CARBOHYD 394
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 406 AA; 46917 MW; D7B7FE8C5493D6C6 CRC64;
MDLRRWFPTL YTQSKDSPVR SRDATLYLLR CVFLMGVRKP PAKFFVAYVL WSFALNFCST
FYQPIGFLTG YISHLSEFSP GEFLTSLQVA FNAWSCSTKV LIVWALVKRF DEANNLLDEM
DRRITDPGER LQIHRAVSLS NRIFFFFMAV YMVYATNTFL SAIFIGRPPY QNYYPFLDWR
SSTLHLALQA GLEYFAMAGA CFQDVCVDCY PVNFVLVLRA HMSIFAERLR RLGTYPYESQ
EQKYERLVQC IQDHKVILRF VDCLRPVISG TIFVQFLVVG LVLGFTLINI VLFANLGSAI
AALSFMAAVL LETTPFCILC NYLTEDCYKL ADALFQSNWI DEEKRYQKTL MYFLQKLQQP
ITFMAMNVFP ISVGTNISVT KFSFSVFTLV KQMNISEKLA KSEMEE
