OR43A_DROME
ID OR43A_DROME Reviewed; 376 AA.
AC P81917; Q9U6X4; Q9V4J0;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2000, sequence version 2.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Odorant receptor 43a;
GN Name=Or43a; Synonyms=AN14, DOR43B.1, dor87, Or43B.1; ORFNames=CG1854;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC STRAIN=Oregon-R; TISSUE=Antenna;
RX PubMed=10089887; DOI=10.1016/s0092-8674(00)80582-6;
RA Vosshall L.B., Amrein H., Morozov P.S., Rzhetsky A., Axel R.;
RT "A spatial map of olfactory receptor expression in the Drosophila
RT antenna.";
RL Cell 96:725-736(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-342.
RX PubMed=10458908; DOI=10.1006/geno.1999.5894;
RA Gao Q., Chess A.;
RT "Identification of candidate Drosophila olfactory receptors from genomic
RT DNA sequence.";
RL Genomics 60:31-39(1999).
RN [5]
RP IDENTIFICATION, AND TISSUE SPECIFICITY.
RX PubMed=10069338; DOI=10.1016/s0896-6273(00)81093-4;
RA Clyne P.J., Warr C.G., Freeman M.R., Lessing D., Kim J., Carlson J.R.;
RT "A novel family of divergent seven-transmembrane proteins: candidate
RT odorant receptors in Drosophila.";
RL Neuron 22:327-338(1999).
RN [6]
RP TISSUE SPECIFICITY.
RX PubMed=10943836; DOI=10.1016/s0092-8674(00)00021-0;
RA Vosshall L.B., Wong A.M., Axel R.;
RT "An olfactory sensory map in the fly brain.";
RL Cell 102:147-159(2000).
RN [7]
RP FUNCTION.
RX PubMed=11481494; DOI=10.1073/pnas.151103998;
RA Wetzel C.H., Behrendt H.J., Gisselmann G., Stortkuhl K.F., Hovemann B.,
RA Hatt H.;
RT "Functional expression and characterization of a Drosophila odorant
RT receptor in a heterologous cell system.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:9377-9380(2001).
RN [8]
RP TISSUE SPECIFICITY, AND FUNCTION.
RX PubMed=11481495; DOI=10.1073/pnas.151105698;
RA Stortkuhl K.F., Kettler R.;
RT "Functional analysis of an olfactory receptor in Drosophila melanogaster.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:9381-9385(2001).
RN [9]
RP TISSUE SPECIFICITY, AND FUNCTION.
RX PubMed=15647466; DOI=10.1093/chemse/bji003;
RA Stortkuhl K.F., Kettler R., Fischer S., Hovemann B.T.;
RT "An increased receptive field of olfactory receptor Or43a in the antennal
RT lobe of Drosophila reduces benzaldehyde-driven avoidance behavior.";
RL Chem. Senses 30:81-87(2005).
RN [10]
RP FUNCTION.
RX PubMed=16615896; DOI=10.1016/j.cell.2006.01.050;
RA Hallem E.A., Carlson J.R.;
RT "Coding of odors by a receptor repertoire.";
RL Cell 125:143-160(2006).
RN [11]
RP FUNCTION, INTERACTION WITH ORCO, AND TISSUE SPECIFICITY.
RX PubMed=16402857; DOI=10.1371/journal.pbio.0040020;
RA Benton R., Sachse S., Michnick S.W., Vosshall L.B.;
RT "Atypical membrane topology and heteromeric function of Drosophila odorant
RT receptors in vivo.";
RL PLoS Biol. 4:E20-E20(2006).
RN [12]
RP FUNCTION.
RX PubMed=20628035; DOI=10.1534/genetics.110.119446;
RA Rollmann S.M., Wang P., Date P., West S.A., Mackay T.F., Anholt R.R.;
RT "Odorant receptor polymorphisms and natural variation in olfactory behavior
RT in Drosophila melanogaster.";
RL Genetics 186:687-697(2010).
CC -!- FUNCTION: Odorant receptor which mediates acceptance or avoidance
CC behavior, depending on its substrates. The odorant receptor repertoire
CC encodes a large collection of odor stimuli that vary widely in
CC identity, intensity, and duration. Complexes with Orco to form odorant-
CC sensing units, providing sensitive and prolonged odorant signaling and
CC calcium permeability. They are necessary and sufficient to promote
CC functional reconstitution of odor-evoked signaling in sensory neurons
CC that normally respond only to carbon dioxide. Involved in the
CC behavioral responses to acetophenone, benzaldehyde, benzyl alcohol,
CC cyclohexanone, and cyclohexanol. {ECO:0000269|PubMed:11481494,
CC ECO:0000269|PubMed:11481495, ECO:0000269|PubMed:15647466,
CC ECO:0000269|PubMed:16402857, ECO:0000269|PubMed:16615896,
CC ECO:0000269|PubMed:20628035}.
CC -!- SUBUNIT: Interacts with Orco. Complexes exist early in the endomembrane
CC system in olfactory sensory neurons (OSNs), coupling these complexes to
CC the conserved ciliary trafficking pathway.
CC {ECO:0000269|PubMed:16402857}.
CC -!- INTERACTION:
CC P81917; Q9VNB5: Orco; NbExp=2; IntAct=EBI-15562282, EBI-15562228;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed with Orco in 20 sensory neurons on the
CC distal edge of the antenna. {ECO:0000269|PubMed:10069338,
CC ECO:0000269|PubMed:10089887, ECO:0000269|PubMed:10943836,
CC ECO:0000269|PubMed:11481495, ECO:0000269|PubMed:15647466,
CC ECO:0000269|PubMed:16402857}.
CC -!- MISCELLANEOUS: The atypical heteromeric and topological design of the
CC odorant receptors appears to be an insect-specific solution for odor
CC recognition, making the OR/Orco complex an attractive target for the
CC development of highly selective insect repellents to disrupt olfactory-
CC mediated host-seeking behaviors of insect disease vectors. Odor-evoked
CC OR currents are independent of known G-protein-coupled second messenger
CC pathways.
CC -!- SIMILARITY: Belongs to the insect chemoreceptor superfamily.
CC Heteromeric odorant receptor channel (TC 1.A.69) family. Or30a
CC subfamily. {ECO:0000305}.
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DR EMBL; AF127926; AAD26361.1; -; mRNA.
DR EMBL; AE013599; AAF59280.1; -; Genomic_DNA.
DR RefSeq; NP_523647.2; NM_078923.3.
DR AlphaFoldDB; P81917; -.
DR SMR; P81917; -.
DR BioGRID; 61527; 2.
DR DIP; DIP-29175N; -.
DR IntAct; P81917; 1.
DR STRING; 7227.FBpp0088122; -.
DR PaxDb; P81917; -.
DR EnsemblMetazoa; FBtr0089051; FBpp0088122; FBgn0026389.
DR GeneID; 35644; -.
DR KEGG; dme:Dmel_CG1854; -.
DR CTD; 35644; -.
DR FlyBase; FBgn0026389; Or43a.
DR VEuPathDB; VectorBase:FBgn0026389; -.
DR eggNOG; ENOG502STQW; Eukaryota.
DR GeneTree; ENSGT00520000056289; -.
DR HOGENOM; CLU_033399_9_0_1; -.
DR InParanoid; P81917; -.
DR OMA; LRMWGDL; -.
DR OrthoDB; 775940at2759; -.
DR PhylomeDB; P81917; -.
DR BioGRID-ORCS; 35644; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 35644; -.
DR PRO; PR:P81917; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0026389; Expressed in antenna and 4 other tissues.
DR ExpressionAtlas; P81917; differential.
DR Genevisible; P81917; DM.
DR GO; GO:0032590; C:dendrite membrane; IDA:FlyBase.
DR GO; GO:0016021; C:integral component of membrane; HDA:FlyBase.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005549; F:odorant binding; ISS:FlyBase.
DR GO; GO:0004984; F:olfactory receptor activity; IDA:FlyBase.
DR GO; GO:0004888; F:transmembrane signaling receptor activity; ISM:FlyBase.
DR GO; GO:0050911; P:detection of chemical stimulus involved in sensory perception of smell; HDA:FlyBase.
DR GO; GO:0007608; P:sensory perception of smell; IEP:FlyBase.
DR GO; GO:0007165; P:signal transduction; IEA:UniProtKB-KW.
DR InterPro; IPR004117; 7tm6_olfct_rcpt.
DR PANTHER; PTHR21137; PTHR21137; 1.
DR Pfam; PF02949; 7tm_6; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Olfaction; Receptor; Reference proteome;
KW Sensory transduction; Transducer; Transmembrane; Transmembrane helix.
FT CHAIN 1..376
FT /note="Odorant receptor 43a"
FT /id="PRO_0000174244"
FT TOPO_DOM 1..32
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 33..53
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 54..58
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 59..79
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 80..123
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 124..144
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 145..178
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 179..199
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 200..247
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 248..268
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 269..276
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 277..297
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 298..342
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 343..363
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 364..376
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT CONFLICT 154
FT /note="L -> V (in Ref. 1; AAD26361)"
FT /evidence="ECO:0000305"
FT CONFLICT 237..272
FT /note="Missing (in Ref. 4)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 376 AA; 43090 MW; 1B3638FE7EA49606 CRC64;
MTIEDIGLVG INVRMWRHLA VLYPTPGSSW RKFAFVLPVT AMNLMQFVYL LRMWGDLPAF
ILNMFFFSAI FNALMRTWLV IIKRRQFEEF LGQLATLFHS ILDSTDEWGR GILRRAEREA
RNLAILNLSA SFLDIVGALV SPLFREERAH PFGLALPGVS MTSSPVYEVI YLAQLPTPLL
LSMMYMPFVS LFAGLAIFGK AMLQILVHRL GQIGGEEQSE EERFQRLASC IAYHTQVMRY
VWQLNKLVAN IVAVEAIIFG SIICSLLFCL NIITSPTQVI SIVMYILTML YVLFTYYNRA
NEICLENNRV AEAVYNVPWY EAGTRFRKTL LIFLMQTQHP MEIRVGNVYP MTLAMFQSLL
NASYSYFTML RGVTGK
