OR43B_DROME
ID OR43B_DROME Reviewed; 403 AA.
AC P81918; Q9U6Y0; Q9V332;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 3.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Odorant receptor 43b;
GN Name=Or43b; Synonyms=AN7, DOR25A.1, OR44A.1; ORFNames=CG17853;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND TISSUE SPECIFICITY.
RX PubMed=10458908; DOI=10.1006/geno.1999.5894;
RA Gao Q., Chess A.;
RT "Identification of candidate Drosophila olfactory receptors from genomic
RT DNA sequence.";
RL Genomics 60:31-39(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP IDENTIFICATION, AND TISSUE SPECIFICITY.
RX PubMed=10069338; DOI=10.1016/s0896-6273(00)81093-4;
RA Clyne P.J., Warr C.G., Freeman M.R., Lessing D., Kim J., Carlson J.R.;
RT "A novel family of divergent seven-transmembrane proteins: candidate
RT odorant receptors in Drosophila.";
RL Neuron 22:327-338(1999).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=10943836; DOI=10.1016/s0092-8674(00)00021-0;
RA Vosshall L.B., Wong A.M., Axel R.;
RT "An olfactory sensory map in the fly brain.";
RL Cell 102:147-159(2000).
RN [6]
RP FUNCTION.
RX PubMed=16615896; DOI=10.1016/j.cell.2006.01.050;
RA Hallem E.A., Carlson J.R.;
RT "Coding of odors by a receptor repertoire.";
RL Cell 125:143-160(2006).
CC -!- FUNCTION: Odorant receptor which mediates acceptance or avoidance
CC behavior, depending on its substrates. The odorant receptor repertoire
CC encodes a large collection of odor stimuli that vary widely in
CC identity, intensity, and duration. May form a complex with Orco to form
CC odorant-sensing units, providing sensitive and prolonged odorant
CC signaling and calcium permeability. {ECO:0000269|PubMed:16615896}.
CC -!- SUBUNIT: Interacts with Orco. Complexes exist early in the endomembrane
CC system in olfactory sensory neurons (OSNs), coupling these complexes to
CC the conserved ciliary trafficking pathway (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in 16 olfactory receptor neurons in a
CC broad area across the antenna, including both anterior and posterior
CC faces and in the maxillary palp. This expression pattern matches the
CC distribution of the small sensilla basiconica. Expression in the
CC antenna is observed late in antennal development at 93 hours APF.
CC {ECO:0000269|PubMed:10069338, ECO:0000269|PubMed:10458908,
CC ECO:0000269|PubMed:10943836}.
CC -!- MISCELLANEOUS: The atypical heteromeric and topological design of the
CC odorant receptors appears to be an insect-specific solution for odor
CC recognition, making the OR/Orco complex an attractive target for the
CC development of highly selective insect repellents to disrupt olfactory-
CC mediated host-seeking behaviors of insect disease vectors. Odor-evoked
CC OR currents are independent of known G-protein-coupled second messenger
CC pathways.
CC -!- SIMILARITY: Belongs to the insect chemoreceptor superfamily.
CC Heteromeric odorant receptor channel (TC 1.A.69) family. Or2a
CC subfamily. {ECO:0000305}.
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DR EMBL; AE013599; AAF59173.2; -; Genomic_DNA.
DR RefSeq; NP_523656.2; NM_078932.3.
DR AlphaFoldDB; P81918; -.
DR SMR; P81918; -.
DR IntAct; P81918; 1.
DR STRING; 7227.FBpp0087962; -.
DR TCDB; 1.A.69.1.1; the heteromeric odorant receptor channel (horc) family.
DR PaxDb; P81918; -.
DR DNASU; 35743; -.
DR EnsemblMetazoa; FBtr0088887; FBpp0087962; FBgn0026393.
DR GeneID; 35743; -.
DR KEGG; dme:Dmel_CG17853; -.
DR CTD; 35743; -.
DR FlyBase; FBgn0026393; Or43b.
DR VEuPathDB; VectorBase:FBgn0026393; -.
DR GeneTree; ENSGT00540000073151; -.
DR HOGENOM; CLU_033399_8_0_1; -.
DR InParanoid; P81918; -.
DR OMA; LFHSAWW; -.
DR OrthoDB; 710105at2759; -.
DR PhylomeDB; P81918; -.
DR BioGRID-ORCS; 35743; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 35743; -.
DR PRO; PR:P81918; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0026393; Expressed in antenna and 12 other tissues.
DR ExpressionAtlas; P81918; baseline and differential.
DR Genevisible; P81918; DM.
DR GO; GO:0032590; C:dendrite membrane; ISS:FlyBase.
DR GO; GO:0016021; C:integral component of membrane; HDA:FlyBase.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005549; F:odorant binding; IMP:FlyBase.
DR GO; GO:0004984; F:olfactory receptor activity; IDA:FlyBase.
DR GO; GO:0050911; P:detection of chemical stimulus involved in sensory perception of smell; IDA:FlyBase.
DR GO; GO:0007608; P:sensory perception of smell; IMP:FlyBase.
DR GO; GO:0007165; P:signal transduction; IEA:UniProtKB-KW.
DR InterPro; IPR004117; 7tm6_olfct_rcpt.
DR PANTHER; PTHR21137; PTHR21137; 1.
DR Pfam; PF02949; 7tm_6; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Membrane; Olfaction; Receptor; Reference proteome;
KW Sensory transduction; Transducer; Transmembrane; Transmembrane helix.
FT CHAIN 1..403
FT /note="Odorant receptor 43b"
FT /id="PRO_0000174245"
FT TOPO_DOM 1..49
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 50..70
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 71..83
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 84..104
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 105..139
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 140..160
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 161..193
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 194..214
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 215..271
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 272..292
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 293..299
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 300..320
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 321..372
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 373..393
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 394..403
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT CONFLICT 259
FT /note="N -> KCS (in Ref. 1)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 403 AA; 46626 MW; 90C55CCBB5C5B94F CRC64;
MFGHFKLVYP APISEPIQSR DSNAYMMETL RNSGLNLKND FGIGRKIWRV FSFTYNMVIL
PVSFPINYVI HLAEFPPELL LQSLQLCLNT WCFALKFFTL IVYTHRLELA NKHFDELDKY
CVKPAEKRKV RDMVATITRL YLTFVVVYVL YATSTLLDGL LHHRVPYNTY YPFINWRVDR
TQMYIQSFLE YFTVGYAIYV ATATDSYPVI YVAALRTHIL LLKDRIIYLG DPSNEGSSDP
SYMFKSLVDC IKAHRTMLNF CDAIQPIISG TIFAQFIICG SILGIIMINM VLFADQSTRF
GIVIYVMAVL LQTFPLCFYC NAIVDDCKEL AHALFHSAWW VQDKRYQRTV IQFLQKLQQP
MTFTAMNIFN INLATNINVA KFAFTVYAIA SGMNLDQKLS IKE
