OR46B_DROME
ID OR46B_DROME Reviewed; 384 AA.
AC Q9V3N2; P81920;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 2.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Odorant receptor 46a, isoform B;
GN Name=Or46a;
GN Synonyms=AN8, AN9, dor19, DOR46F, DOR46F.1, Or46b, OR46F, Or46F.1, Or46F.2;
GN ORFNames=CG33478;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=10458908; DOI=10.1006/geno.1999.5894;
RA Gao Q., Chess A.;
RT "Identification of candidate Drosophila olfactory receptors from genomic
RT DNA sequence.";
RL Genomics 60:31-39(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP IDENTIFICATION, AND TISSUE SPECIFICITY.
RX PubMed=10069338; DOI=10.1016/s0896-6273(00)81093-4;
RA Clyne P.J., Warr C.G., Freeman M.R., Lessing D., Kim J., Carlson J.R.;
RT "A novel family of divergent seven-transmembrane proteins: candidate
RT odorant receptors in Drosophila.";
RL Neuron 22:327-338(1999).
RN [5]
RP IDENTIFICATION.
RC STRAIN=Oregon-R; TISSUE=Antenna;
RX PubMed=10089887; DOI=10.1016/s0092-8674(00)80582-6;
RA Vosshall L.B., Amrein H., Morozov P.S., Rzhetsky A., Axel R.;
RT "A spatial map of olfactory receptor expression in the Drosophila
RT antenna.";
RL Cell 96:725-736(1999).
RN [6]
RP IDENTIFICATION OF ALTERNATIVE SPLICING.
RX PubMed=14608037; DOI=10.1073/pnas.2335847100;
RA Robertson H.M., Warr C.G., Carlson J.R.;
RT "Molecular evolution of the insect chemoreceptor gene superfamily in
RT Drosophila melanogaster.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:14537-14542(2003).
CC -!- FUNCTION: Odorant receptor which mediates acceptance or avoidance
CC behavior, depending on its substrates. The odorant receptor repertoire
CC encodes a large collection of odor stimuli that vary widely in
CC identity, intensity, and duration. May form a complex with Orco to form
CC odorant-sensing units, providing sensitive and prolonged odorant
CC signaling and calcium permeability (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Interacts with Orco. Complexes exist early in the endomembrane
CC system in olfactory sensory neurons (OSNs), coupling these complexes to
CC the conserved ciliary trafficking pathway (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=B;
CC IsoId=Q9V3N2-1; Sequence=Displayed;
CC Name=A;
CC IsoId=P81919-1; Sequence=External;
CC -!- TISSUE SPECIFICITY: Isoform B is expressed in the antenna.
CC {ECO:0000269|PubMed:10069338}.
CC -!- MISCELLANEOUS: The atypical heteromeric and topological design of the
CC odorant receptors appears to be an insect-specific solution for odor
CC recognition, making the OR/Orco complex an attractive target for the
CC development of highly selective insect repellents to disrupt olfactory-
CC mediated host-seeking behaviors of insect disease vectors. Odor-evoked
CC OR currents are independent of known G-protein-coupled second messenger
CC pathways.
CC -!- SIMILARITY: Belongs to the insect chemoreceptor superfamily.
CC Heteromeric odorant receptor channel (TC 1.A.69) family. Or2a
CC subfamily. {ECO:0000305}.
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DR EMBL; AE013599; AAM68774.1; -; Genomic_DNA.
DR RefSeq; NP_995793.1; NM_206071.2. [Q9V3N2-1]
DR AlphaFoldDB; Q9V3N2; -.
DR SMR; Q9V3N2; -.
DR IntAct; Q9V3N2; 1.
DR STRING; 7227.FBpp0089408; -.
DR GlyGen; Q9V3N2; 2 sites.
DR PaxDb; Q9V3N2; -.
DR EnsemblMetazoa; FBtr0088331; FBpp0089408; FBgn0026388. [Q9V3N2-1]
DR GeneID; 2768728; -.
DR KEGG; dme:Dmel_CG33478; -.
DR CTD; 2768728; -.
DR FlyBase; FBgn0026388; Or46a.
DR VEuPathDB; VectorBase:FBgn0026388; -.
DR eggNOG; ENOG502SV87; Eukaryota.
DR GeneTree; ENSGT00530000064740; -.
DR HOGENOM; CLU_033399_6_3_1; -.
DR InParanoid; Q9V3N2; -.
DR PhylomeDB; Q9V3N2; -.
DR BioGRID-ORCS; 2768728; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 2768728; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0026388; Expressed in maxillary palp olfactory receptor neuron (Drosophila) and 2 other tissues.
DR ExpressionAtlas; Q9V3N2; differential.
DR Genevisible; Q9V3N2; DM.
DR GO; GO:0032590; C:dendrite membrane; ISS:FlyBase.
DR GO; GO:0016021; C:integral component of membrane; ISM:FlyBase.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005549; F:odorant binding; ISS:FlyBase.
DR GO; GO:0004984; F:olfactory receptor activity; IMP:FlyBase.
DR GO; GO:0050911; P:detection of chemical stimulus involved in sensory perception of smell; IMP:FlyBase.
DR GO; GO:0007608; P:sensory perception of smell; ISS:FlyBase.
DR GO; GO:0007165; P:signal transduction; IEA:UniProtKB-KW.
DR InterPro; IPR004117; 7tm6_olfct_rcpt.
DR PANTHER; PTHR21137; PTHR21137; 1.
DR Pfam; PF02949; 7tm_6; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cell membrane; Glycoprotein; Membrane; Olfaction;
KW Receptor; Reference proteome; Sensory transduction; Transducer;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..384
FT /note="Odorant receptor 46a, isoform B"
FT /id="PRO_0000174249"
FT TOPO_DOM 1..37
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 38..58
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 59..65
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 66..86
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 87..130
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 131..151
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 152..165
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 166..186
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 187..247
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 248..268
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 269..283
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 284..304
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 305..348
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 349..369
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 370..384
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT CARBOHYD 59
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 370
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 384 AA; 44451 MW; B86E9D7F6CC665C4 CRC64;
MVTEDFYKYQ VWYFQILGVW QLPTWAADHQ RRFQSMRFGF ILVILFIMLL LFSFEMLNNI
SQVREILKVF FMFATEISCM AKLLHLKLKS RKLAGLVDAM LSPEFGVKSE QEMQMLELDR
VAVVRMRNSY GIMSLGAASL ILIVPCFDNF GELPLAMLEV CSIEGWICYW SQYLFHSICL
LPTCVLNITY DSVAYSLLCF LKVQLQMLVL RLEKLGPVIE PQDNEKIAME LRECAAYYNR
IVRFKDLVEL FIKGPGSVQL MCSVLVLVSN LYDMSTMSIA NGDAIFMLKT CIYQLVMLWQ
IFIICYASNE VTVQSSRLCH SIYSSQWTGW NRANRRIVLL MMQRFNSPML LSTFNPTFAF
SLEAFGSIVN CSYSYFALLK RVNS
