OR47A_DROME
ID OR47A_DROME Reviewed; 385 AA.
AC P81921; Q9U6X8;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1999, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Odorant receptor 47a;
GN Name=Or47a; Synonyms=AN10, dor24, DOR47E.1, Or47E.1; ORFNames=CG13225;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Oregon-R; TISSUE=Antenna;
RX PubMed=10089887; DOI=10.1016/s0092-8674(00)80582-6;
RA Vosshall L.B., Amrein H., Morozov P.S., Rzhetsky A., Axel R.;
RT "A spatial map of olfactory receptor expression in the Drosophila
RT antenna.";
RL Cell 96:725-736(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=10458908; DOI=10.1006/geno.1999.5894;
RA Gao Q., Chess A.;
RT "Identification of candidate Drosophila olfactory receptors from genomic
RT DNA sequence.";
RL Genomics 60:31-39(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5]
RP IDENTIFICATION, AND TISSUE SPECIFICITY.
RX PubMed=10069338; DOI=10.1016/s0896-6273(00)81093-4;
RA Clyne P.J., Warr C.G., Freeman M.R., Lessing D., Kim J., Carlson J.R.;
RT "A novel family of divergent seven-transmembrane proteins: candidate
RT odorant receptors in Drosophila.";
RL Neuron 22:327-338(1999).
RN [6]
RP TISSUE SPECIFICITY.
RX PubMed=10943836; DOI=10.1016/s0092-8674(00)00021-0;
RA Vosshall L.B., Wong A.M., Axel R.;
RT "An olfactory sensory map in the fly brain.";
RL Cell 102:147-159(2000).
RN [7]
RP FUNCTION.
RX PubMed=16615896; DOI=10.1016/j.cell.2006.01.050;
RA Hallem E.A., Carlson J.R.;
RT "Coding of odors by a receptor repertoire.";
RL Cell 125:143-160(2006).
RN [8]
RP FUNCTION, INTERACTION WITH ORCO, AND TISSUE SPECIFICITY.
RX PubMed=16402857; DOI=10.1371/journal.pbio.0040020;
RA Benton R., Sachse S., Michnick S.W., Vosshall L.B.;
RT "Atypical membrane topology and heteromeric function of Drosophila odorant
RT receptors in vivo.";
RL PLoS Biol. 4:E20-E20(2006).
RN [9]
RP FUNCTION, AND INTERACTION WITH ORCO.
RX PubMed=18408712; DOI=10.1038/nature06850;
RA Sato K., Pellegrino M., Nakagawa T., Nakagawa T., Vosshall L.B.,
RA Touhara K.;
RT "Insect olfactory receptors are heteromeric ligand-gated ion channels.";
RL Nature 452:1002-1006(2008).
RN [10]
RP FUNCTION, AND INTERACTION WITH ORCO.
RX PubMed=18408711; DOI=10.1038/nature06861;
RA Wicher D., Schaefer R., Bauernfeind R., Stensmyr M.C., Heller R.,
RA Heinemann S.H., Hansson B.S.;
RT "Drosophila odorant receptors are both ligand-gated and cyclic-nucleotide-
RT activated cation channels.";
RL Nature 452:1007-1011(2008).
RN [11]
RP FUNCTION.
RX PubMed=21613503; DOI=10.1523/jneurosci.6254-10.2011;
RA Montague S.A., Mathew D., Carlson J.R.;
RT "Similar odorants elicit different behavioral and physiological responses,
RT some supersustained.";
RL J. Neurosci. 31:7891-7899(2011).
RN [12]
RP FUNCTION.
RX PubMed=21857978; DOI=10.1371/journal.pone.0022996;
RA Hoare D.J., Humble J., Jin D., Gilding N., Petersen R., Cobb M.,
RA McCrohan C.;
RT "Modeling peripheral olfactory coding in Drosophila larvae.";
RL PLoS ONE 6:E22996-E22996(2011).
RN [13]
RP FUNCTION.
RX PubMed=22038943; DOI=10.1093/chemse/bjr097;
RA Richgels P.K., Rollmann S.M.;
RT "Genetic variation in odorant receptors contributes to variation in
RT olfactory behavior in a natural population of Drosophila melanogaster.";
RL Chem. Senses 37:229-240(2012).
CC -!- FUNCTION: Odorant receptor which mediates acceptance or avoidance
CC behavior, depending on its substrates. The odorant receptor repertoire
CC encodes a large collection of odor stimuli that vary widely in
CC identity, intensity, and duration. Complexes with Orco to form odorant-
CC sensing units, providing sensitive and prolonged odorant signaling and
CC calcium permeability. They are necessary and sufficient to promote
CC functional reconstitution of odor-evoked signaling in sensory neurons
CC that normally respond only to carbon dioxide. Involved in the
CC behavioral responses to esters. Involved in the behavioral responses to
CC pentyl acetate. {ECO:0000269|PubMed:16402857,
CC ECO:0000269|PubMed:16615896, ECO:0000269|PubMed:18408711,
CC ECO:0000269|PubMed:18408712, ECO:0000269|PubMed:21613503,
CC ECO:0000269|PubMed:21857978, ECO:0000269|PubMed:22038943}.
CC -!- SUBUNIT: Interacts with Orco. Complexes exist early in the endomembrane
CC system in olfactory sensory neurons (OSNs), coupling these complexes to
CC the conserved ciliary trafficking pathway.
CC {ECO:0000269|PubMed:16402857, ECO:0000269|PubMed:18408711,
CC ECO:0000269|PubMed:18408712}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed with Orco in 40 olfactory receptor
CC neurons in a broad area across the antenna, including both anterior and
CC posterior faces. This expression pattern matches the distribution of
CC the small sensilla basiconica. Expression in the antenna is observed
CC late in antennal development at 93 hours APF.
CC {ECO:0000269|PubMed:10069338, ECO:0000269|PubMed:10943836,
CC ECO:0000269|PubMed:16402857}.
CC -!- MISCELLANEOUS: The atypical heteromeric and topological design of the
CC odorant receptors appears to be an insect-specific solution for odor
CC recognition, making the OR/Orco complex an attractive target for the
CC development of highly selective insect repellents to disrupt olfactory-
CC mediated host-seeking behaviors of insect disease vectors. Odor-evoked
CC OR currents are independent of known G-protein-coupled second messenger
CC pathways.
CC -!- SIMILARITY: Belongs to the insect chemoreceptor superfamily.
CC Heteromeric odorant receptor channel (TC 1.A.69) family. Or1a
CC subfamily. {ECO:0000305}.
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DR EMBL; AF156880; AAD39006.1; -; mRNA.
DR EMBL; AE013599; AAF58695.1; -; Genomic_DNA.
DR RefSeq; NP_523689.1; NM_078965.3.
DR AlphaFoldDB; P81921; -.
DR SMR; P81921; -.
DR STRING; 7227.FBpp0087212; -.
DR PaxDb; P81921; -.
DR EnsemblMetazoa; FBtr0088111; FBpp0087212; FBgn0026386.
DR GeneID; 36187; -.
DR KEGG; dme:Dmel_CG13225; -.
DR CTD; 36187; -.
DR FlyBase; FBgn0026386; Or47a.
DR VEuPathDB; VectorBase:FBgn0026386; -.
DR eggNOG; ENOG502SSPN; Eukaryota.
DR GeneTree; ENSGT00940000168837; -.
DR HOGENOM; CLU_033399_7_1_1; -.
DR InParanoid; P81921; -.
DR OMA; RITGYFF; -.
DR OrthoDB; 957822at2759; -.
DR PhylomeDB; P81921; -.
DR BioGRID-ORCS; 36187; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 36187; -.
DR PRO; PR:P81921; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0026386; Expressed in antennal basiconic sensillum ab5 (Drosophila) and 5 other tissues.
DR ExpressionAtlas; P81921; differential.
DR Genevisible; P81921; DM.
DR GO; GO:0030425; C:dendrite; IDA:FlyBase.
DR GO; GO:0032590; C:dendrite membrane; ISS:FlyBase.
DR GO; GO:0016021; C:integral component of membrane; HDA:FlyBase.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005549; F:odorant binding; ISS:FlyBase.
DR GO; GO:0004984; F:olfactory receptor activity; IDA:FlyBase.
DR GO; GO:0004888; F:transmembrane signaling receptor activity; ISM:FlyBase.
DR GO; GO:0050911; P:detection of chemical stimulus involved in sensory perception of smell; HDA:FlyBase.
DR GO; GO:0007608; P:sensory perception of smell; IEP:FlyBase.
DR GO; GO:0007165; P:signal transduction; IEA:UniProtKB-KW.
DR InterPro; IPR004117; 7tm6_olfct_rcpt.
DR PANTHER; PTHR21137; PTHR21137; 1.
DR Pfam; PF02949; 7tm_6; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Olfaction; Receptor; Reference proteome;
KW Sensory transduction; Transducer; Transmembrane; Transmembrane helix.
FT CHAIN 1..385
FT /note="Odorant receptor 47a"
FT /id="PRO_0000174250"
FT TOPO_DOM 1..33
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 34..54
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 55..62
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 63..83
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 84..129
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 130..150
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 151..175
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 176..196
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 197..255
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 256..276
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 277..284
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 285..305
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 306..357
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 358..378
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 379..385
FT /note="Extracellular"
FT /evidence="ECO:0000255"
SQ SEQUENCE 385 AA; 43966 MW; 498DA16E50A86038 CRC64;
MDSFLQVQKS TIALLGFDLF SENREMWKRP YRAMNVFSIA AIFPFILAAV LHNWKNVLLL
ADAMVALLIT ILGLFKFSMI LYLRRDFKRL IDKFRLLMSN EAEQGEEYAE ILNAANKQDQ
RMCTLFRTCF LLAWALNSVL PLVRMGLSYW LAGHAEPELP FPCLFPWNIH IIRNYVLSFI
WSAFASTGVV LPAVSLDTIF CSFTSNLCAF FKIAQYKVVR FKGGSLKESQ ATLNKVFALY
QTSLDMCNDL NQCYQPIICA QFFISSLQLC MLGYLFSITF AQTEGVYYAS FIATIIIQAY
IYCYCGENLK TESASFEWAI YDSPWHESLG AGGASTSICR SLLISMMRAH RGFRITGYFF
EANMEAFSSI VRTAMSYITM LRSFS
