OR47B_DROME
ID OR47B_DROME Reviewed; 412 AA.
AC P81922; Q9V5X4;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2004, sequence version 2.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Odorant receptor 47b;
GN Name=Or47b; Synonyms=DOR47E.2, Or47E.2; ORFNames=CG13206;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP TISSUE SPECIFICITY.
RX PubMed=10069338; DOI=10.1016/s0896-6273(00)81093-4;
RA Clyne P.J., Warr C.G., Freeman M.R., Lessing D., Kim J., Carlson J.R.;
RT "A novel family of divergent seven-transmembrane proteins: candidate
RT odorant receptors in Drosophila.";
RL Neuron 22:327-338(1999).
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=10943836; DOI=10.1016/s0092-8674(00)00021-0;
RA Vosshall L.B., Wong A.M., Axel R.;
RT "An olfactory sensory map in the fly brain.";
RL Cell 102:147-159(2000).
RN [5]
RP FUNCTION.
RX PubMed=16615896; DOI=10.1016/j.cell.2006.01.050;
RA Hallem E.A., Carlson J.R.;
RT "Coding of odors by a receptor repertoire.";
RL Cell 125:143-160(2006).
RN [6]
RP FUNCTION.
RX PubMed=21516101; DOI=10.1038/nn.2800;
RA Wang L., Han X., Mehren J., Hiroi M., Billeter J.C., Miyamoto T.,
RA Amrein H., Levine J.D., Anderson D.J.;
RT "Hierarchical chemosensory regulation of male-male social interactions in
RT Drosophila.";
RL Nat. Neurosci. 14:757-762(2011).
RN [7]
RP FUNCTION.
RX PubMed=22476771; DOI=10.1177/0748730411434384;
RA Lone S.R., Sharma V.K.;
RT "Or47b receptor neurons mediate sociosexual interactions in the fruit fly
RT Drosophila melanogaster.";
RL J. Biol. Rhythms 27:107-116(2012).
RN [8]
RP FUNCTION.
RX PubMed=23652013; DOI=10.1038/ncomms2837;
RA Sakurai A., Koganezawa M., Yasunaga K., Emoto K., Yamamoto D.;
RT "Select interneuron clusters determine female sexual receptivity in
RT Drosophila.";
RL Nat. Commun. 4:1825-1825(2013).
CC -!- FUNCTION: Odorant receptor which mediates acceptance or avoidance
CC behavior, depending on its substrates. The odorant receptor repertoire
CC encodes a large collection of odor stimuli that vary widely in
CC identity, intensity, and duration. May form a complex with Orco to form
CC odorant-sensing units, providing sensitive and prolonged odorant
CC signaling and calcium permeability. Plays an important role in
CC sociosexual interactions since its enhances courtship in a pheromone-
CC dependent manner. {ECO:0000269|PubMed:16615896,
CC ECO:0000269|PubMed:21516101, ECO:0000269|PubMed:22476771,
CC ECO:0000269|PubMed:23652013}.
CC -!- SUBUNIT: Interacts with Orco. Complexes exist early in the endomembrane
CC system in olfactory sensory neurons (OSNs), coupling these complexes to
CC the conserved ciliary trafficking pathway (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in olfactory sensory neurons in the
CC antenna. {ECO:0000269|PubMed:10069338, ECO:0000269|PubMed:10943836}.
CC -!- MISCELLANEOUS: The atypical heteromeric and topological design of the
CC odorant receptors appears to be an insect-specific solution for odor
CC recognition, making the OR/Orco complex an attractive target for the
CC development of highly selective insect repellents to disrupt olfactory-
CC mediated host-seeking behaviors of insect disease vectors. Odor-evoked
CC OR currents are independent of known G-protein-coupled second messenger
CC pathways.
CC -!- SIMILARITY: Belongs to the insect chemoreceptor superfamily.
CC Heteromeric odorant receptor channel (TC 1.A.69) family. Or49a
CC subfamily. {ECO:0000305}.
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DR EMBL; AE013599; AAF58667.3; -; Genomic_DNA.
DR RefSeq; NP_523690.3; NM_078966.3.
DR AlphaFoldDB; P81922; -.
DR SMR; P81922; -.
DR STRING; 7227.FBpp0087262; -.
DR PaxDb; P81922; -.
DR EnsemblMetazoa; FBtr0088166; FBpp0087262; FBgn0026385.
DR GeneID; 36212; -.
DR KEGG; dme:Dmel_CG13206; -.
DR CTD; 36212; -.
DR FlyBase; FBgn0026385; Or47b.
DR VEuPathDB; VectorBase:FBgn0026385; -.
DR eggNOG; ENOG502SSXX; Eukaryota.
DR GeneTree; ENSGT00940000166470; -.
DR HOGENOM; CLU_055891_1_0_1; -.
DR InParanoid; P81922; -.
DR OMA; FTLGTYM; -.
DR OrthoDB; 622712at2759; -.
DR PhylomeDB; P81922; -.
DR BioGRID-ORCS; 36212; 1 hit in 1 CRISPR screen.
DR GenomeRNAi; 36212; -.
DR PRO; PR:P81922; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0026385; Expressed in antenna.
DR Genevisible; P81922; DM.
DR GO; GO:0032590; C:dendrite membrane; ISS:FlyBase.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005549; F:odorant binding; ISS:FlyBase.
DR GO; GO:0004984; F:olfactory receptor activity; HDA:FlyBase.
DR GO; GO:0050911; P:detection of chemical stimulus involved in sensory perception of smell; HDA:FlyBase.
DR GO; GO:0007608; P:sensory perception of smell; NAS:UniProtKB.
DR GO; GO:0007165; P:signal transduction; IEA:UniProtKB-KW.
DR InterPro; IPR004117; 7tm6_olfct_rcpt.
DR PANTHER; PTHR21137; PTHR21137; 1.
DR Pfam; PF02949; 7tm_6; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Membrane; Olfaction; Receptor; Reference proteome;
KW Sensory transduction; Transducer; Transmembrane; Transmembrane helix.
FT CHAIN 1..412
FT /note="Odorant receptor 47b"
FT /id="PRO_0000174251"
FT TOPO_DOM 1..74
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 75..95
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 96..103
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 104..124
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 125..169
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 170..190
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 191..229
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 230..250
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 251..302
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 303..323
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 324..330
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 331..351
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 352..389
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 390..410
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 411..412
FT /note="Extracellular"
FT /evidence="ECO:0000255"
SQ SEQUENCE 412 AA; 47913 MW; F49279703127DB3F CRC64;
MNDSGYQSNL SLLRVFLDEF RSVLRQESPG LIPRLAFYYV RAFLSLLCQY PNKKLASLPL
YRWINLFIMC NVMTIFWTMF VALPESKNVI EMGDDLVWIS GMALVFTKIF YMHLRCDEID
ELISDFEYYN RELRPHNIDE EVLGWQRLCY VIESGLYINC FCLVNFFSAA IFLQPLLGEG
KLPFHSVYPF QWHRLDLHPY TFWFLYIWQS LTSQHNLMSI LMVDMVGIST FLQTALNLKL
LCIEIRKLGD MEVSDKRFHE EFCRVVRFHQ HIIKLVGKAN RAFNGAFNAQ LMASFSLISI
STFETMAAAA VDPKMAAKFV LLMLVAFIQL SLWCVSGTLV YTQSVEVAQA AFDINDWHTK
SPGIQRDISF VILRAQKPLM YVAEPFLPFT LGTYMLVLKN CYRLLALMQE SM
