OR4_AEDAE
ID OR4_AEDAE Reviewed; 406 AA.
AC Q16EI9; X2D0Z8; X2D176; X2D1A9; X2D1B0; X2D1E2; X2D1N6;
DT 04-FEB-2015, integrated into UniProtKB/Swiss-Prot.
DT 25-JUL-2006, sequence version 1.
DT 25-MAY-2022, entry version 91.
DE RecName: Full=Odorant receptor 4 {ECO:0000303|PubMed:25391959};
DE Short=AaegOr4;
GN Name=GPROR4 {ECO:0000303|PubMed:25391959};
GN Synonyms=Or4 {ECO:0000303|PubMed:25391959};
GN ORFNames=AAEL015147 {ECO:0000312|EMBL:EAT32646.1};
OS Aedes aegypti (Yellowfever mosquito) (Culex aegypti).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Nematocera; Culicoidea; Culicidae;
OC Culicinae; Aedini; Aedes; Stegomyia.
OX NCBI_TaxID=7159;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], VARIANTS ARG-3; ILE-5; GLU-8; ARG-12; ILE-27;
RP THR-35; SER-42; TYR-69; ILE-75; THR-104; ASP-118; GLU-124; TYR-128;
RP SER-166; LYS-172; PRO-187; PRO-189; ARG-249; ARG-252; GLN-259; ASP-269;
RP ILE-306; PHE-330; VAL-331; VAL-338; ARG-339; 344-ASN--PRO-355 DELINS
RP LYS-TRP-TYR-GLN-LEU-GLU-ALA-SER-GLN-GLN-ASN-SER; ALA-358; 367-GLN--ARG-370
RP DELINS ARG-ALA-GLN-THR; ARG-402 AND ARG-406, FUNCTION, AND POLYMORPHISM.
RX PubMed=25391959; DOI=10.1038/nature13964;
RA McBride C.S., Baier F., Omondi A.B., Spitzer S.A., Lutomiah J., Sang R.,
RA Ignell R., Vosshall L.B.;
RT "Evolution of mosquito preference for humans linked to an odorant
RT receptor.";
RL Nature 515:222-227(2014).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LVPib12;
RX PubMed=17510324; DOI=10.1126/science.1138878;
RA Nene V., Wortman J.R., Lawson D., Haas B.J., Kodira C.D., Tu Z.J.,
RA Loftus B.J., Xi Z., Megy K., Grabherr M., Ren Q., Zdobnov E.M., Lobo N.F.,
RA Campbell K.S., Brown S.E., Bonaldo M.F., Zhu J., Sinkins S.P.,
RA Hogenkamp D.G., Amedeo P., Arensburger P., Atkinson P.W., Bidwell S.L.,
RA Biedler J., Birney E., Bruggner R.V., Costas J., Coy M.R., Crabtree J.,
RA Crawford M., DeBruyn B., DeCaprio D., Eiglmeier K., Eisenstadt E.,
RA El-Dorry H., Gelbart W.M., Gomes S.L., Hammond M., Hannick L.I.,
RA Hogan J.R., Holmes M.H., Jaffe D., Johnston S.J., Kennedy R.C., Koo H.,
RA Kravitz S., Kriventseva E.V., Kulp D., Labutti K., Lee E., Li S.,
RA Lovin D.D., Mao C., Mauceli E., Menck C.F., Miller J.R., Montgomery P.,
RA Mori A., Nascimento A.L., Naveira H.F., Nusbaum C., O'Leary S.B., Orvis J.,
RA Pertea M., Quesneville H., Reidenbach K.R., Rogers Y.-H.C., Roth C.W.,
RA Schneider J.R., Schatz M., Shumway M., Stanke M., Stinson E.O.,
RA Tubio J.M.C., Vanzee J.P., Verjovski-Almeida S., Werner D., White O.R.,
RA Wyder S., Zeng Q., Zhao Q., Zhao Y., Hill C.A., Raikhel A.S., Soares M.B.,
RA Knudson D.L., Lee N.H., Galagan J., Salzberg S.L., Paulsen I.T.,
RA Dimopoulos G., Collins F.H., Bruce B., Fraser-Liggett C.M., Severson D.W.;
RT "Genome sequence of Aedes aegypti, a major arbovirus vector.";
RL Science 316:1718-1723(2007).
CC -!- FUNCTION: Odorant receptor that specifically recognizes the human
CC odorant sulcatone (6-methylhept-5-en-2-onesul), a volatile odorant
CC emitted at uniquely high levels in humans, thereby playing a key role
CC in mosquito's preference in biting human compared to other animals.
CC {ECO:0000269|PubMed:25391959}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- POLYMORPHISM: The protein-coding sequence is highly variable and 7
CC major alleles have been identified, numbered from allele A to G.
CC Alleles A, B, C, F, and G are highly sensitive to sulcatone, while
CC alleles D and E are less sensitive to sulcatone odor. The sequence
CC displayed is that of allele A. {ECO:0000269|PubMed:25391959}.
CC -!- MISCELLANEOUS: Expressed at much higher levels in mosquito strains that
CC preferentially bite human compared to 'ancestral' strains that prefer
CC to bite non-human animals. {ECO:0000269|PubMed:25391959}.
CC -!- SIMILARITY: Belongs to the heteromeric odorant receptor channel (TC
CC 1.A.69) family. {ECO:0000255|RuleBase:RU351113, ECO:0000305}.
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DR EMBL; KF801614; AHF20361.1; -; mRNA.
DR EMBL; KF801615; AHF20362.1; -; mRNA.
DR EMBL; KF801617; AHF20363.1; -; mRNA.
DR EMBL; KF801618; AHF20364.1; -; mRNA.
DR EMBL; KF801619; AHF20365.1; -; mRNA.
DR EMBL; KF801620; AHF20366.1; -; mRNA.
DR EMBL; KF801621; AHF20367.1; -; mRNA.
DR EMBL; CH478696; EAT32646.1; -; Genomic_DNA.
DR RefSeq; XP_001650792.1; XM_001650742.1.
DR AlphaFoldDB; Q16EI9; -.
DR SMR; Q16EI9; -.
DR STRING; 7159.AAEL015147-PA; -.
DR PRIDE; Q16EI9; -.
DR GeneID; 5566400; -.
DR KEGG; aag:5566400; -.
DR CTD; 5566400; -.
DR VEuPathDB; VectorBase:AAEL015147; -.
DR eggNOG; ENOG502SSPN; Eukaryota.
DR HOGENOM; CLU_033399_0_1_1; -.
DR InParanoid; Q16EI9; -.
DR OMA; DCLFCIL; -.
DR OrthoDB; 957822at2759; -.
DR PhylomeDB; Q16EI9; -.
DR Proteomes; UP000008820; Unassembled WGS sequence.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005549; F:odorant binding; IEA:InterPro.
DR GO; GO:0004984; F:olfactory receptor activity; IEA:InterPro.
DR GO; GO:0007165; P:signal transduction; IEA:UniProtKB-KW.
DR InterPro; IPR004117; 7tm6_olfct_rcpt.
DR PANTHER; PTHR21137; PTHR21137; 1.
DR Pfam; PF02949; 7tm_6; 1.
PE 2: Evidence at transcript level;
KW Membrane; Olfaction; Receptor; Reference proteome; Sensory transduction;
KW Transducer; Transmembrane; Transmembrane helix.
FT CHAIN 1..406
FT /note="Odorant receptor 4"
FT /id="PRO_0000431949"
FT TOPO_DOM 1..43
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 44..64
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 65..78
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 79..99
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 100..136
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 137..157
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 158..167
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 168..188
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 189..276
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 277..297
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 298..301
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 302..322
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 323..370
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 371..391
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 392..406
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT VARIANT 3
FT /note="Q -> R (in allele E)"
FT /evidence="ECO:0000269|PubMed:25391959"
FT VARIANT 5
FT /note="L -> I (in allele C, allele D, allele E, allele F
FT and allele G)"
FT /evidence="ECO:0000269|PubMed:25391959"
FT VARIANT 8
FT /note="D -> E (in allele C, allele D and allele G)"
FT /evidence="ECO:0000269|PubMed:25391959"
FT VARIANT 12
FT /note="G -> R (in allele E and allele F)"
FT /evidence="ECO:0000269|PubMed:25391959"
FT VARIANT 27
FT /note="L -> I (in allele E)"
FT /evidence="ECO:0000269|PubMed:25391959"
FT VARIANT 35
FT /note="A -> T (in allele F)"
FT /evidence="ECO:0000269|PubMed:25391959"
FT VARIANT 42
FT /note="A -> S (in allele B)"
FT /evidence="ECO:0000269|PubMed:25391959"
FT VARIANT 69
FT /note="H -> Y (in allele D, allele E, allele F and allele
FT G)"
FT /evidence="ECO:0000269|PubMed:25391959"
FT VARIANT 75
FT /note="V -> I (in allele E)"
FT /evidence="ECO:0000269|PubMed:25391959"
FT VARIANT 103
FT /note="R -> H (in allele G)"
FT /evidence="ECO:0000269|PubMed:25391959"
FT VARIANT 104
FT /note="A -> T (in allele G)"
FT /evidence="ECO:0000269|PubMed:25391959"
FT VARIANT 118
FT /note="N -> D (in allele D)"
FT /evidence="ECO:0000269|PubMed:25391959"
FT VARIANT 124
FT /note="D -> E (in allele D, allele E and allele G)"
FT /evidence="ECO:0000269|PubMed:25391959"
FT VARIANT 128
FT /note="S -> Y (in allele E)"
FT /evidence="ECO:0000269|PubMed:25391959"
FT VARIANT 166
FT /note="G -> S (in allele C)"
FT /evidence="ECO:0000269|PubMed:25391959"
FT VARIANT 172
FT /note="E -> K (in allele F)"
FT /evidence="ECO:0000269|PubMed:25391959"
FT VARIANT 187
FT /note="S -> P (in allele C, allele D, allele E, allele F
FT and allele G)"
FT /evidence="ECO:0000269|PubMed:25391959"
FT VARIANT 189
FT /note="H -> P (in allele C, allele F and allele G)"
FT /evidence="ECO:0000269|PubMed:25391959"
FT VARIANT 249
FT /note="Q -> R (in allele C, allele D, allele E, allele F
FT and allele G)"
FT /evidence="ECO:0000269|PubMed:25391959"
FT VARIANT 252
FT /note="K -> R (in allele G)"
FT /evidence="ECO:0000269|PubMed:25391959"
FT VARIANT 259
FT /note="R -> Q (in allele E)"
FT /evidence="ECO:0000269|PubMed:25391959"
FT VARIANT 269
FT /note="N -> D (in allele G)"
FT /evidence="ECO:0000269|PubMed:25391959"
FT VARIANT 306
FT /note="V -> I (in allele G)"
FT /evidence="ECO:0000269|PubMed:25391959"
FT VARIANT 330
FT /note="L -> F (in allele G)"
FT /evidence="ECO:0000269|PubMed:25391959"
FT VARIANT 331
FT /note="A -> V (in allele C, allele D, allele E and allele
FT F)"
FT /evidence="ECO:0000269|PubMed:25391959"
FT VARIANT 338
FT /note="A -> V (in allele G)"
FT /evidence="ECO:0000269|PubMed:25391959"
FT VARIANT 339
FT /note="G -> R (in allele F)"
FT /evidence="ECO:0000269|PubMed:25391959"
FT VARIANT 344..355
FT /note="NWYQSKVSQQNP -> KWYQLEASQQNS (in allele G)"
FT /evidence="ECO:0000269|PubMed:25391959"
FT VARIANT 358
FT /note="G -> A (in allele E)"
FT /evidence="ECO:0000269|PubMed:25391959"
FT VARIANT 367..370
FT /note="QGQR -> RAQT (in allele G)"
FT /evidence="ECO:0000269|PubMed:25391959"
FT VARIANT 402
FT /note="K -> R (in allele C, allele D, allele E, allele F
FT and allele G)"
FT /evidence="ECO:0000269|PubMed:25391959"
FT VARIANT 406
FT /note="H -> R (in allele C and allele G)"
FT /evidence="ECO:0000269|PubMed:25391959"
SQ SEQUENCE 406 AA; 46099 MW; 74A6421EA11BA6A9 CRC64;
MTQSLEFDQT FGFIAKVLQM IGYPSCLAPY PTTFASRLKS SAGFVVCFLM LTYCVFGQII
NIGLLMMGHR QTDQVVEEVA IQVSSTGFCI IGLAKMYSLS YNRAILSWLI ADFRVKWNAG
ELTDKDRSIR DGTLRPTVAI TTVAALGNII MVSAFNFQPV VEMIYGRVVT GEWVKLFPYV
IWFPFNSTHG AIYYLVYLFE VYSGVIVAVG NVGFNCIFCL LTSHLSMQLK LLCSWIEDMV
EVEDEKGVQS KKKLYRIVRY HQDLIRGRNA LQSMFSTTLF LNFSASSVLM CMQLYLITTA
GITLMVKFTL FMLCILMEIF ILCYYGEEIL ANSSSIAAGA FNSNWYQSKV SQQNPRFGKN
LIPIIQQGQR PMVLTAWKFW PITIRTFSAI LQTSWSYFTL LKTVMH
