OR65A_DROME
ID OR65A_DROME Reviewed; 417 AA.
AC P82982;
DT 01-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Odorant receptor 65a;
GN Name=Or65a; ORFNames=CG32401;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP TISSUE SPECIFICITY.
RX PubMed=10943836; DOI=10.1016/s0092-8674(00)00021-0;
RA Vosshall L.B., Wong A.M., Axel R.;
RT "An olfactory sensory map in the fly brain.";
RL Cell 102:147-159(2000).
RN [4]
RP FUNCTION.
RX PubMed=16615896; DOI=10.1016/j.cell.2006.01.050;
RA Hallem E.A., Carlson J.R.;
RT "Coding of odors by a receptor repertoire.";
RL Cell 125:143-160(2006).
RN [5]
RP FUNCTION.
RX PubMed=21685916; DOI=10.1038/nn.2836;
RA Liu W., Liang X., Gong J., Yang Z., Zhang Y.H., Zhang J.X., Rao Y.;
RT "Social regulation of aggression by pheromonal activation of Or65a
RT olfactory neurons in Drosophila.";
RL Nat. Neurosci. 14:896-902(2011).
CC -!- FUNCTION: Odorant receptor which mediates acceptance or avoidance
CC behavior, depending on its substrates. The odorant receptor repertoire
CC encodes a large collection of odor stimuli that vary widely in
CC identity, intensity, and duration. May form a complex with Orco to form
CC odorant-sensing units, providing sensitive and prolonged odorant
CC signaling and calcium permeability. Involved in olfactory communication
CC for modulating aggression through the sensing of the male-specific
CC pheromone 11-cis-vaccenyl acetate (cVA). Although acute exposure to cVA
CC elicites aggression through Or67d olfactory receptor neurons (ORNs),
CC chronic cVA exposure reduces aggression through Or65a ORNs. Moreover,
CC cVA leads to generalized learning with mated females. It is a major
CC component of the male cuticular hydrocarbon profile, but it is not
CC found on virgin females. During copulation, cVA is transferred to the
CC female in ejaculate along with sperm and peptides that decrease her
CC sexual receptivity. {ECO:0000269|PubMed:16615896,
CC ECO:0000269|PubMed:21685916}.
CC -!- SUBUNIT: Interacts with Orco. Complexes exist early in the endomembrane
CC system in olfactory sensory neurons (OSNs), coupling these complexes to
CC the conserved ciliary trafficking pathway (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in olfactory sensory neurons in the
CC antenna. {ECO:0000269|PubMed:10943836}.
CC -!- MISCELLANEOUS: The atypical heteromeric and topological design of the
CC odorant receptors appears to be an insect-specific solution for odor
CC recognition, making the OR/Orco complex an attractive target for the
CC development of highly selective insect repellents to disrupt olfactory-
CC mediated host-seeking behaviors of insect disease vectors. Odor-evoked
CC OR currents are independent of known G-protein-coupled second messenger
CC pathways.
CC -!- SIMILARITY: Belongs to the insect chemoreceptor superfamily.
CC Heteromeric odorant receptor channel (TC 1.A.69) family. Or49a
CC subfamily. {ECO:0000305}.
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DR EMBL; AE014296; AAN12091.1; -; Genomic_DNA.
DR RefSeq; NP_729161.1; NM_168163.1.
DR AlphaFoldDB; P82982; -.
DR SMR; P82982; -.
DR STRING; 7227.FBpp0076712; -.
DR PaxDb; P82982; -.
DR EnsemblMetazoa; FBtr0077004; FBpp0076712; FBgn0041625.
DR GeneID; 318011; -.
DR KEGG; dme:Dmel_CG32401; -.
DR CTD; 318011; -.
DR FlyBase; FBgn0041625; Or65a.
DR VEuPathDB; VectorBase:FBgn0041625; -.
DR eggNOG; ENOG502SXXU; Eukaryota.
DR GeneTree; ENSGT00940000166470; -.
DR HOGENOM; CLU_055891_1_0_1; -.
DR InParanoid; P82982; -.
DR OMA; EDIYHWS; -.
DR OrthoDB; 622712at2759; -.
DR PhylomeDB; P82982; -.
DR BioGRID-ORCS; 318011; 0 hits in 1 CRISPR screen.
DR ChiTaRS; Or65a; fly.
DR GenomeRNAi; 318011; -.
DR PRO; PR:P82982; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0041625; Expressed in antenna and 1 other tissue.
DR ExpressionAtlas; P82982; differential.
DR Genevisible; P82982; DM.
DR GO; GO:0032590; C:dendrite membrane; ISS:FlyBase.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005549; F:odorant binding; ISS:FlyBase.
DR GO; GO:0004984; F:olfactory receptor activity; HDA:FlyBase.
DR GO; GO:0050911; P:detection of chemical stimulus involved in sensory perception of smell; HDA:FlyBase.
DR GO; GO:0007165; P:signal transduction; IEA:UniProtKB-KW.
DR InterPro; IPR004117; 7tm6_olfct_rcpt.
DR PANTHER; PTHR21137; PTHR21137; 1.
DR Pfam; PF02949; 7tm_6; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Membrane; Olfaction; Receptor; Reference proteome;
KW Sensory transduction; Transducer; Transmembrane; Transmembrane helix.
FT CHAIN 1..417
FT /note="Odorant receptor 65a"
FT /id="PRO_0000174259"
FT TOPO_DOM 1..62
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 63..83
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 84..98
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 99..119
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 120..152
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 153..173
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 174..206
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 207..227
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 228..290
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 291..311
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 312..316
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 317..337
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 338..393
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 394..414
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 415..417
FT /note="Extracellular"
FT /evidence="ECO:0000255"
SQ SEQUENCE 417 AA; 48730 MW; 2112BE9E1E356059 CRC64;
MTELRSERKN GNWDRLFGPF FESWAVFKAP QAKSRHIIAY WTRDQLKALG FYMNSEQRRL
PRIVAWQYFV SIQLATALAS LFYGISESIG DIVNLGRDLV FIITIIFICF RLVFFAQYAG
ELDVIIDALE DIYHWSIKGP ATKEVQETKR LHFLLFMALI ITWFSFLILF MLIKISTPFW
IESQTLPFHV SWPFQLHDPS KHPIAYIIIF VSQSTTMLYF LIWLGVVENM GVSLFFELTS
ALRVLCIELR NLQELCLGDE DMLYRELCRM TKFHQQIILL TDRCNHIFNG AFIMQMLINF
LLVSLSLFEV LAAKKNPQVA VEYMIIMLMT LGHLSFWSKF GDMFSKESEQ VALAVYEAYD
PNVGSKSIHR QFCFFIQRAQ KPLIMKASPF PPFNLENYMF ILKQCYSILT ILANTLE
