ID   A2MGH_ECOLI             Reviewed;        1367 AA.
AC   P76464; P76465;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   27-SEP-2017, sequence version 3.
DT   25-MAY-2022, entry version 118.
DE   RecName: Full=Putative alpha-2-macroglobulin homolog {ECO:0000305};
DE   Flags: Precursor;
GN   Name=yfaS; Synonyms=yfaR; OrderedLocusNames=b4500, JW2221/JW2222;
GN   ORFNames=b2227/b2228;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=9205837; DOI=10.1093/dnares/4.2.91;
RA   Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T.,
RA   Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K.,
RA   Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T., Oyama S.,
RA   Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H.,
RA   Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.;
RT   "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12
RT   genome corresponding to 50.0-68.8 min on the linkage map and analysis of
RT   its sequence features.";
RL   DNA Res. 4:91-113(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
CC   -!- SIMILARITY: Belongs to the protease inhibitor I39 (alpha-2-
CC       macroglobulin) family. Bacterial alpha-2-macroglobulin subfamily.
CC       {ECO:0000305}.
CC   -!- CAUTION: Could be the product of a pseudogene, it is missing the C-
CC       terminus compared to orthologs. {ECO:0000305}.
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DR   EMBL; U00096; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AP009048; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   AlphaFoldDB; P76464; -.
DR   SMR; P76464; -.
DR   DIP; DIP-11951N; -.
DR   IntAct; P76464; 25.
DR   PRIDE; P76464; -.
DR   EchoBASE; EB3834; -.
DR   InParanoid; P76464; -.
DR   PhylomeDB; P76464; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0004866; F:endopeptidase inhibitor activity; IEA:InterPro.
DR   Gene3D; 2.60.40.10; -; 1.
DR   InterPro; IPR011625; A2M_N_BRD.
DR   InterPro; IPR013783; Ig-like_fold.
DR   InterPro; IPR001599; Macroglobln_a2.
DR   InterPro; IPR002890; MG2.
DR   InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR   Pfam; PF00207; A2M; 1.
DR   Pfam; PF07703; A2M_BRD; 1.
DR   Pfam; PF01835; MG2; 1.
DR   SMART; SM01360; A2M; 1.
DR   SMART; SM01359; A2M_N_2; 1.
DR   SUPFAM; SSF48239; SSF48239; 1.
PE   5: Uncertain;
KW   Reference proteome; Signal.
FT   SIGNAL          1..38
FT                   /evidence="ECO:0000255"
FT   CHAIN           39..1367
FT                   /note="Putative alpha-2-macroglobulin homolog"
FT                   /id="PRO_0000036239"
SQ   SEQUENCE   1367 AA;  151114 MW;  41ED70F6A73271DB CRC64;
     MDTQRFQSQF HWHLSFKFSG AIAACLSLSL VGTGLANADD SLPSSNYAPP AGGTFFLLAD
     SSFSSSEEAK VRLEAPGRDY RRYQMEEYGG VDVRLYRIPD PMAFLRQQKN LHRIVVQPQY
     LGDGLNNTLT WLWDNWYGKS RRVMQRTFSS QSRQNVTQAL PELQLGNAII KPSRYVQNNQ
     FSPLKKYPLV KQFRYPLWQA KPFEPQQGVK LEGASSNFIS PQPGNIYIPL GQQEPGLYLV
     EAMVGGYRAT TVVFVSDTVA LSKVSGKELL VWTAGKKQGE AKPGSEILWT DGLGVMTRGV
     TDDSGTLQLQ HISPERSYIL GKDAEGGVFV SENFFYESEI YNTRLYIFTD RPLYRAGDRV
     DVKVIGREFH DPLHSSPIVS APAKLSVLDA NGSLLQTVNV TLDARNGGQG SFRLPENAVA
     GGYELRLAYR NQVYSSSFRV ANYIKPHFEI GLALAKKEFK TGEAVSGKLQ LLYPDGEPVK
     NARVQLSLRA QQLSMVGNDL RYAGRFPVSL EGSETVSDAS GHVALNLPAA DKPSRYLLTV
     SASDGAAYRV TTTKEILIER GLAHYSLSTA AQYSNSGESV VFRYAALESS KQVPVTYEWL
     RLEDRTSHSG ELPSGGKSFT VNFAKPGNYN LTLRDKDGLI LAGLSHAVSG KGSTAHTGTV
     DIVADKTLYQ PGETAKMLIT FPEPIDEALL TLERDRVEQQ SLLSHPANWL TLQRLNDTQY
     EARVPVSNSF APNITFSVLY TRNGQYSFQN AGIKVAVPQL DIRVKTDKTH YQPGELVNVE
     LTSSLKGKPV SAQLTVGVVD EMIYALQPEI APNIGKFFYP LGRNNVRTSS SLSFISYDQA
     LSSEPVAPGA TNRSERRVKM LERPRREEVD TAAWMPSLTT DKQGKAYFTF LMPDSLTRWR
     ITARGMNGDG LVGQGRAYLR SEKNLYMKWS MPTVYRVGDK PAAGLFIFSQ QDNEPVALVT
     KFAGAEMRQT LTLHKGANYI SLTQNIQQSG LLSAELQQNG QVQDSISTKL SFVDNSWPVE
     QQKNVMLGGG DNALMLPEQA SNIRLQSSET PQEIFRNNLD ALVDEPWGGV INTGSRLIPL
     SLAWRSLADH QSAAANDIRQ MIQDNRLRLM QLAGPGARFT WWGEDGNGDA FLTAWAWYAD
     WQASQAIGVT QQPEYWQHML DSYAEQADNM PLLHRALVLA WAQEMNLPCK TLLKGLDEAI
     ARRGTKTEDF SEEDTRDIND SLILDTPESP LADAVANVLT MTLLKKAQLK STVMPQVQQY
     AWDKAANSNQ PLAHTVVLLN SGGDATQTAA ILSGLTAEQS TIERALAMNW LAKYMATMPP
     VVLPAPAGAW AKHKLTGGGE DWRWVGQGVP DILSFGDELS PQNVQVR