ID   ASAF_ASPFN              Reviewed;          83 AA.
AC   B8N0F2;
DT   20-JUN-2018, integrated into UniProtKB/Swiss-Prot.
DT   03-MAR-2009, sequence version 1.
DT   03-AUG-2022, entry version 33.
DE   RecName: Full=Aspergillic acid biosynthesis cluster protein F {ECO:0000303|PubMed:29674152};
GN   Name=asaF {ECO:0000303|PubMed:29674152}; ORFNames=AFLA_023060;
OS   Aspergillus flavus (strain ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357
OS   / JCM 12722 / SRRC 167).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Circumdati.
OX   NCBI_TaxID=332952;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 200026 / FGSC A1120 / IAM 13836 / NRRL 3357 / JCM 12722 / SRRC
RC   167;
RX   PubMed=25883274; DOI=10.1128/genomea.00168-15;
RA   Nierman W.C., Yu J., Fedorova-Abrams N.D., Losada L., Cleveland T.E.,
RA   Bhatnagar D., Bennett J.W., Dean R., Payne G.A.;
RT   "Genome sequence of Aspergillus flavus NRRL 3357, a strain that causes
RT   aflatoxin contamination of food and feed.";
RL   Genome Announc. 3:E0016815-E0016815(2015).
RN   [2]
RP   IDENTIFICATION WITHIN THE CLUSTER, AND INDUCTION.
RX   PubMed=23834374; DOI=10.1111/mpp.12056;
RA   Dolezal A.L., Obrian G.R., Nielsen D.M., Woloshuk C.P., Boston R.S.,
RA   Payne G.A.;
RT   "Localization, morphology and transcriptional profile of Aspergillus flavus
RT   during seed colonization.";
RL   Mol. Plant Pathol. 14:898-909(2013).
RN   [3]
RP   FUNCTION, DISRUPTION PHENOTYPE, AND PATHWAY.
RX   PubMed=29674152; DOI=10.1016/j.fgb.2018.04.009;
RA   Lebar M.D., Cary J.W., Majumdar R., Carter-Wientjes C.H., Mack B.M.,
RA   Wei Q., Uka V., De Saeger S., Diana Di Mavungu J.;
RT   "Identification and functional analysis of the aspergillic acid gene
RT   cluster in Aspergillus flavus.";
RL   Fungal Genet. Biol. 116:14-23(2018).
CC   -!- FUNCTION: Part of the gene cluster that mediates the biosynthesis of
CC       aspergillic acid, a hydroxamic acid-containing pyrazinone with
CC       aliphatic side chains that originates from leucine (Leu) and isoleucine
CC       (Ile) (PubMed:29674152). Aspergillic acid has antibiotic properties and
CC       was shown to be lethal to mice (PubMed:29674152). The first step in the
CC       pathway is the production of deoxyaspergillic acid via a condensation
CC       between the Ile amine and the Leu carboxylic acid, followed by a
CC       reductive release from the protein forming the dipeptide aldehyde
CC       NH(2)-Leu-Ile-CHO, which could undergo an intermolecular cyclization
CC       resulting in a dihydropyrazinone (PubMed:29674152). As the NRPS asaC
CC       lacks a condensation domain, it is improbable that it is responsible
CC       for condensation of Leu and Ile (PubMed:29674152). One possibility is
CC       that asaC acts on a previously condensed dipeptide and functions as a
CC       Leu-Ile reductase to yield deoxyaspergillic acid (PubMed:29674152).
CC       After asaC forms deoxyaspergillic acid, the cytochrome P450 asaD
CC       oxidizes the pyrazinone to the hydroxamic acid-containing bioactive
CC       metabolite aspergillic acid (PubMed:29674152). The
CC       hydroxylase/desaturase asaB can then convert aspergillic acid to
CC       hydroxyaspergillic acid (PubMed:29674152). Both aspergillic acid and
CC       hydroxyaspergillic acid can form complexes with iron producing
CC       ferriaspergillin analogs (PubMed:29674152).
CC       {ECO:0000269|PubMed:29674152}.
CC   -!- PATHWAY: Secondary metabolite biosynthesis.
CC       {ECO:0000305|PubMed:29674152}.
CC   -!- INDUCTION: Expressed during the earliest stages of maize kernel
CC       infection (PubMed:23834374). {ECO:0000269|PubMed:23834374}.
CC   -!- DISRUPTION PHENOTYPE: Does not affect ferriaspergillin production
CC       (PubMed:29674152). {ECO:0000269|PubMed:29674152}.
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DR   EMBL; EQ963473; EED55035.1; -; Genomic_DNA.
DR   RefSeq; XP_002373817.1; XM_002373776.1.
DR   AlphaFoldDB; B8N0F2; -.
DR   EnsemblFungi; EED55035; EED55035; AFLA_023060.
DR   VEuPathDB; FungiDB:AFLA_023060; -.
DR   HOGENOM; CLU_2542154_0_0_1; -.
DR   Proteomes; UP000001875; Unassembled WGS sequence.
PE   2: Evidence at transcript level;
FT   CHAIN           1..83
FT                   /note="Aspergillic acid biosynthesis cluster protein F"
FT                   /id="PRO_0000444458"
SQ   SEQUENCE   83 AA;  9194 MW;  18E16A8C3CD39418 CRC64;
     MSPTITRPLI GQLCPLTNVG QIAETFSSEE VSFVETVDPL RHASKGGHHD DDNLPQGTLN
     NIWRGNPVIA WAALLRIAHK HFR