OR85A_DROME
ID OR85A_DROME Reviewed; 397 AA.
AC Q9VHS4;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Odorant receptor 85a;
GN Name=Or85a; ORFNames=CG7454;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP TISSUE SPECIFICITY.
RX PubMed=10943836; DOI=10.1016/s0092-8674(00)00021-0;
RA Vosshall L.B., Wong A.M., Axel R.;
RT "An olfactory sensory map in the fly brain.";
RL Cell 102:147-159(2000).
RN [4]
RP FUNCTION.
RX PubMed=16615896; DOI=10.1016/j.cell.2006.01.050;
RA Hallem E.A., Carlson J.R.;
RT "Coding of odors by a receptor repertoire.";
RL Cell 125:143-160(2006).
CC -!- FUNCTION: Odorant receptor which mediates acceptance or avoidance
CC behavior, depending on its substrates. The odorant receptor repertoire
CC encodes a large collection of odor stimuli that vary widely in
CC identity, intensity, and duration. May form a complex with Orco to form
CC odorant-sensing units, providing sensitive and prolonged odorant
CC signaling and calcium permeability. {ECO:0000269|PubMed:16615896}.
CC -!- SUBUNIT: Interacts with Orco. Complexes exist early in the endomembrane
CC system in olfactory sensory neurons (OSNs), coupling these complexes to
CC the conserved ciliary trafficking pathway (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in olfactory sensory neurons in the
CC antenna. {ECO:0000269|PubMed:10943836}.
CC -!- MISCELLANEOUS: The atypical heteromeric and topological design of the
CC odorant receptors appears to be an insect-specific solution for odor
CC recognition, making the OR/Orco complex an attractive target for the
CC development of highly selective insect repellents to disrupt olfactory-
CC mediated host-seeking behaviors of insect disease vectors. Odor-evoked
CC OR currents are independent of known G-protein-coupled second messenger
CC pathways.
CC -!- SIMILARITY: Belongs to the insect chemoreceptor superfamily.
CC Heteromeric odorant receptor channel (TC 1.A.69) family. Or2a
CC subfamily. {ECO:0000305}.
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DR EMBL; AE014297; AAF54225.1; -; Genomic_DNA.
DR RefSeq; NP_524277.1; NM_079553.1.
DR AlphaFoldDB; Q9VHS4; -.
DR SMR; Q9VHS4; -.
DR BioGRID; 66172; 1.
DR DIP; DIP-22791N; -.
DR IntAct; Q9VHS4; 1.
DR STRING; 7227.FBpp0081326; -.
DR GlyGen; Q9VHS4; 1 site.
DR PaxDb; Q9VHS4; -.
DR PRIDE; Q9VHS4; -.
DR EnsemblMetazoa; FBtr0081836; FBpp0081326; FBgn0037576.
DR GeneID; 40991; -.
DR KEGG; dme:Dmel_CG7454; -.
DR CTD; 40991; -.
DR FlyBase; FBgn0037576; Or85a.
DR VEuPathDB; VectorBase:FBgn0037576; -.
DR GeneTree; ENSGT00540000073151; -.
DR HOGENOM; CLU_033399_8_0_1; -.
DR InParanoid; Q9VHS4; -.
DR OMA; LIVEYGN; -.
DR OrthoDB; 733024at2759; -.
DR PhylomeDB; Q9VHS4; -.
DR BioGRID-ORCS; 40991; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 40991; -.
DR PRO; PR:Q9VHS4; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0037576; Expressed in antenna and 2 other tissues.
DR ExpressionAtlas; Q9VHS4; baseline and differential.
DR Genevisible; Q9VHS4; DM.
DR GO; GO:0032590; C:dendrite membrane; ISS:FlyBase.
DR GO; GO:0016021; C:integral component of membrane; HDA:FlyBase.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005549; F:odorant binding; ISS:FlyBase.
DR GO; GO:0004984; F:olfactory receptor activity; HDA:FlyBase.
DR GO; GO:0050911; P:detection of chemical stimulus involved in sensory perception of smell; HDA:FlyBase.
DR GO; GO:0007165; P:signal transduction; IEA:UniProtKB-KW.
DR InterPro; IPR004117; 7tm6_olfct_rcpt.
DR PANTHER; PTHR21137; PTHR21137; 1.
DR Pfam; PF02949; 7tm_6; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Glycoprotein; Membrane; Olfaction; Receptor;
KW Reference proteome; Sensory transduction; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..397
FT /note="Odorant receptor 85a"
FT /id="PRO_0000174274"
FT TOPO_DOM 1..46
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 47..67
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 68..83
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 84..104
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 105..142
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 143..163
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 164..192
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 193..213
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 214..262
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 263..283
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 284..294
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 295..315
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 316..347
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 348..368
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 369..397
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT CARBOHYD 75
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 397 AA; 45820 MW; 4ED07645226497C2 CRC64;
MIFKYIQEPV LGSLFRSRDS LIYLNRSIDQ MGWRLPPRTK PYWWLYYIWT LVVIVLVFIF
IPYGLIMTGI KEFKNFTTTD LFTYVQVPVN TNASIMKGII VLFMRRRFSR AQKMMDAMDI
RCTKMEEKVQ VHRAAALCNR VVVIYHCIYF GYLSMALTGA LVIGKTPFCL YNPLVNPDDH
FYLATAIESV TMAGIILANL ILDVYPIIYV VVLRIHMELL SERIKTLRTD VEKGDDQHYA
ELVECVKDHK LIVEYGNTLR PMISATMFIQ LLSVGLLLGL AAVSMQFYNT VMERVVSGVY
TIAILSQTFP FCYVCEQLSS DCESLTNTLF HSKWIGAERR YRTTMLYFIH NVQQSILFTA
GGIFPICLNT NIKMAKFAFS VVTIVNEMDL AEKLRRE
