OR85B_DROME
ID OR85B_DROME Reviewed; 390 AA.
AC Q9VHQ7;
DT 01-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 2.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Odorant receptor 85b;
GN Name=Or85b; ORFNames=CG11735;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP TISSUE SPECIFICITY.
RX PubMed=10943836; DOI=10.1016/s0092-8674(00)00021-0;
RA Vosshall L.B., Wong A.M., Axel R.;
RT "An olfactory sensory map in the fly brain.";
RL Cell 102:147-159(2000).
RN [4]
RP FUNCTION.
RX PubMed=16615896; DOI=10.1016/j.cell.2006.01.050;
RA Hallem E.A., Carlson J.R.;
RT "Coding of odors by a receptor repertoire.";
RL Cell 125:143-160(2006).
RN [5]
RP INTERACTION WITH ORCO, FUNCTION, DOMAIN, AND MUTAGENESIS OF PHE-142;
RP ASN-143; CYS-208 AND CYS-278.
RX PubMed=20147286; DOI=10.1074/jbc.m109.058321;
RA Nichols A.S., Luetje C.W.;
RT "Transmembrane segment 3 of Drosophila melanogaster odorant receptor
RT subunit 85b contributes to ligand-receptor interactions.";
RL J. Biol. Chem. 285:11854-11862(2010).
CC -!- FUNCTION: Odorant receptor which mediates acceptance or avoidance
CC behavior, depending on its substrates. The odorant receptor repertoire
CC encodes a large collection of odor stimuli that vary widely in
CC identity, intensity, and duration. Forms a complex with Orco to form
CC odorant-sensing units, providing sensitive and prolonged odorant
CC signaling and calcium permeability. Involved in the behavioral
CC responses to 2-heptanone, amyl acetate, and butyl acetate.
CC {ECO:0000269|PubMed:16615896, ECO:0000269|PubMed:20147286}.
CC -!- SUBUNIT: Interacts with Orco. Complexes exist early in the endomembrane
CC system in olfactory sensory neurons (OSNs), coupling these complexes to
CC the conserved ciliary trafficking pathway.
CC {ECO:0000269|PubMed:20147286}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in olfactory sensory neurons in the
CC antenna. {ECO:0000269|PubMed:10943836}.
CC -!- DOMAIN: Residues 146 to 150 play a role in odorant (2-heptanone)
CC activation. {ECO:0000269|PubMed:20147286}.
CC -!- MISCELLANEOUS: The atypical heteromeric and topological design of the
CC odorant receptors appears to be an insect-specific solution for odor
CC recognition, making the OR/Orco complex an attractive target for the
CC development of highly selective insect repellents to disrupt olfactory-
CC mediated host-seeking behaviors of insect disease vectors. Odor-evoked
CC OR currents are independent of known G-protein-coupled second messenger
CC pathways.
CC -!- SIMILARITY: Belongs to the insect chemoreceptor superfamily.
CC Heteromeric odorant receptor channel (TC 1.A.69) family. Or49a
CC subfamily. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE014297; AAF54244.2; -; Genomic_DNA.
DR RefSeq; NP_524279.2; NM_079555.2.
DR AlphaFoldDB; Q9VHQ7; -.
DR SMR; Q9VHQ7; -.
DR IntAct; Q9VHQ7; 3.
DR STRING; 7227.FBpp0081383; -.
DR GlyGen; Q9VHQ7; 1 site.
DR PaxDb; Q9VHQ7; -.
DR EnsemblMetazoa; FBtr0081900; FBpp0081383; FBgn0037590.
DR GeneID; 41007; -.
DR KEGG; dme:Dmel_CG11735; -.
DR CTD; 41007; -.
DR FlyBase; FBgn0037590; Or85b.
DR VEuPathDB; VectorBase:FBgn0037590; -.
DR eggNOG; ENOG502SR0T; Eukaryota.
DR GeneTree; ENSGT00560000077544; -.
DR HOGENOM; CLU_033399_0_0_1; -.
DR InParanoid; Q9VHQ7; -.
DR OMA; MEYWVYD; -.
DR OrthoDB; 957822at2759; -.
DR PhylomeDB; Q9VHQ7; -.
DR BioGRID-ORCS; 41007; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 41007; -.
DR PRO; PR:Q9VHQ7; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0037590; Expressed in antennal basiconic sensillum ab3 (Drosophila) and 3 other tissues.
DR ExpressionAtlas; Q9VHQ7; baseline and differential.
DR Genevisible; Q9VHQ7; DM.
DR GO; GO:0032590; C:dendrite membrane; ISS:FlyBase.
DR GO; GO:0016021; C:integral component of membrane; HDA:FlyBase.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005549; F:odorant binding; IPI:FlyBase.
DR GO; GO:0004984; F:olfactory receptor activity; HDA:FlyBase.
DR GO; GO:0050911; P:detection of chemical stimulus involved in sensory perception of smell; HDA:FlyBase.
DR GO; GO:0007165; P:signal transduction; IEA:UniProtKB-KW.
DR InterPro; IPR004117; 7tm6_olfct_rcpt.
DR PANTHER; PTHR21137; PTHR21137; 1.
DR Pfam; PF02949; 7tm_6; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Glycoprotein; Membrane; Olfaction; Receptor;
KW Reference proteome; Sensory transduction; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..390
FT /note="Odorant receptor 85b"
FT /id="PRO_0000174275"
FT TOPO_DOM 1..30
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 31..51
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 52..66
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 67..87
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 88..126
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 127..147
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 148..200
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 201..221
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 222..260
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 261..281
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 282..291
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 292..312
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 313..360
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 361..381
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 382..390
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT CARBOHYD 179
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT MUTAGEN 142
FT /note="F->C: Leads to altered odorant preference."
FT /evidence="ECO:0000269|PubMed:20147286"
FT MUTAGEN 143
FT /note="N->C: Leads to altered odorant preference."
FT /evidence="ECO:0000269|PubMed:20147286"
FT MUTAGEN 208
FT /note="C->S: Increases odorant sensitivity."
FT /evidence="ECO:0000269|PubMed:20147286"
FT MUTAGEN 278
FT /note="C->S: Increases odorant sensitivity."
FT /evidence="ECO:0000269|PubMed:20147286"
SQ SEQUENCE 390 AA; 45665 MW; A0E338F8838AC3F3 CRC64;
MEKLMKYASF FYTAVGIRPY TNGEESKMNK LIFHIVFWSN VINLSFVGLF ESIYVYSAFM
DNKFLEAVTA LSYIGFVTVG MSKMFFIRWK KTAITELINE LKEIYPNGLI REERYNLPMY
LGTCSRISLI YSLLYSVLIW TFNLFCVMEY WVYDKWLNIR VVGKQLPYLM YIPWKWQDNW
SYYPLLFSQN FAGYTSAAGQ ISTDVLLCAV ATQLVMHFDF LSNSMERHEL SGDWKKDSRF
LVDIVRYHER ILRLSDAVND IFGIPLLLNF MVSSFVICFV GFQMTVGVPP DIVVKLFLFL
VSSMSQVYLI CHYGQLVADA SYGFSVATYN QKWYKADVRY KRALVIIIAR SQKVTFLKAT
IFLDITRSTM TDLLQISYKF FALLRTMYTQ
