ORA1_DANRE
ID ORA1_DANRE Reviewed; 316 AA.
AC A0A0R4IM31; A8E0W7; B3DJV6; Q58QC6; Q597G5;
DT 20-JUN-2018, integrated into UniProtKB/Swiss-Prot.
DT 20-JAN-2016, sequence version 1.
DT 03-AUG-2022, entry version 32.
DE RecName: Full=Olfactory receptor class A-like protein 1 {ECO:0000305};
DE AltName: Full=Vomeronasal type-1 receptor-like protein {ECO:0000303|PubMed:15809442};
GN Name=ora1 {ECO:0000305}; Synonyms=Vlr2 {ECO:0000303|PubMed:15809442};
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955 {ECO:0000312|Proteomes:UP000000437};
RN [1] {ECO:0000312|EMBL:AAQ88216.1}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND TISSUE SPECIFICITY.
RX PubMed=15809442; DOI=10.1073/pnas.0402581102;
RA Pfister P., Rodriguez I.;
RT "Olfactory expression of a single and highly variable V1r pheromone
RT receptor-like gene in fish species.";
RL Proc. Natl. Acad. Sci. U.S.A. 102:5489-5494(2005).
RN [2] {ECO:0000312|Proteomes:UP000000437}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen;
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [3] {ECO:0000312|EMBL:AAI63621.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RG NIH - Zebrafish Gene Collection (ZGC) project;
RL Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000312|EMBL:CAO85673.1}
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1-174, AND TISSUE SPECIFICITY.
RC TISSUE=Olfactory rosette {ECO:0000312|EMBL:CAO85673.1};
RX PubMed=17717047; DOI=10.1101/gr.6553207;
RA Saraiva L.R., Korsching S.I.;
RT "A novel olfactory receptor gene family in teleost fish.";
RL Genome Res. 17:1448-1457(2007).
RN [5] {ECO:0000305}
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=24831010; DOI=10.1074/jbc.m114.573162;
RA Behrens M., Frank O., Rawel H., Ahuja G., Potting C., Hofmann T.,
RA Meyerhof W., Korsching S.;
RT "ORA1, a zebrafish olfactory receptor ancestral to all mammalian V1R genes,
RT recognizes 4-hydroxyphenylacetic acid, a putative reproductive pheromone.";
RL J. Biol. Chem. 289:19778-19788(2014).
CC -!- FUNCTION: Probable pheromone receptor. Shows high specificity for 4-
CC hydroxyphenylacetic acid. Activation of the receptor stimulates
CC intracellular calcium release. {ECO:0000269|PubMed:24831010}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|RuleBase:RU364061,
CC ECO:0000269|PubMed:24831010}; Multi-pass membrane protein
CC {ECO:0000255|RuleBase:RU364061}.
CC -!- TISSUE SPECIFICITY: Highly expressed in the olfactory rosette where it
CC localizes to a subset of olfactory sensory neurons, mainly in the
CC apical region of the neuroepithelium. Not detected in other tissues
CC tested. {ECO:0000269|PubMed:15809442, ECO:0000269|PubMed:17717047}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000255|RuleBase:RU364061}.
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DR EMBL; AY279524; AAQ88216.1; -; Genomic_DNA.
DR EMBL; AY900117; AAX54938.1; -; Genomic_DNA.
DR EMBL; CABZ01099332; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC163621; AAI63621.1; -; mRNA.
DR EMBL; AM778163; CAO85673.1; -; mRNA.
DR RefSeq; NP_001124140.1; NM_001130668.1.
DR AlphaFoldDB; A0A0R4IM31; -.
DR SMR; A0A0R4IM31; -.
DR BindingDB; A0A0R4IM31; -.
DR Ensembl; ENSDART00000171954; ENSDARP00000135478; ENSDARG00000098601.
DR GeneID; 553133; -.
DR KEGG; dre:553133; -.
DR CTD; 553133; -.
DR ZFIN; ZDB-GENE-050510-2; ora1.
DR GeneTree; ENSGT01030000234553; -.
DR OMA; LCVTIKG; -.
DR OrthoDB; 1219728at2759; -.
DR PRO; PR:A0A0R4IM31; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 22.
DR Bgee; ENSDARG00000098601; Expressed in olfactory rosette and 3 other tissues.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:ZFIN.
DR GO; GO:0033293; F:monocarboxylic acid binding; IDA:ZFIN.
DR GO; GO:0005550; F:pheromone binding; IDA:ZFIN.
DR GO; GO:0016503; F:pheromone receptor activity; ISS:ZFIN.
DR GO; GO:0072545; F:tyrosine binding; IDA:ZFIN.
DR GO; GO:0019236; P:response to pheromone; IEA:UniProtKB-KW.
DR GO; GO:0007608; P:sensory perception of smell; IEA:UniProtKB-KW.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR004072; Vmron_rcpt_1.
DR PANTHER; PTHR24062; PTHR24062; 1.
DR Pfam; PF03402; V1R; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW Membrane; Olfaction; Pheromone response; Pheromone-binding; Receptor;
KW Reference proteome; Sensory transduction; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..316
FT /note="Olfactory receptor class A-like protein 1"
FT /id="PRO_0000444571"
FT TOPO_DOM 1..8
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 9..29
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 30..39
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 40..60
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 61..97
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 98..118
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 119..132
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 133..153
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 154..187
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 188..208
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 209..233
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 234..254
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 255..264
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 265..285
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 286..316
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT CARBOHYD 156
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 176
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT DISULFID 81..169
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT CONFLICT 121
FT /note="V -> L (in Ref. 3; AAI63621)"
FT /evidence="ECO:0000305"
FT CONFLICT 266..267
FT /note="AD -> GH (in Ref. 1; AAX54938)"
FT /evidence="ECO:0000305"
FT CONFLICT 267
FT /note="D -> H (in Ref. 1; AAQ88216)"
FT /evidence="ECO:0000305"
FT CONFLICT 280
FT /note="S -> I (in Ref. 1; AAX54938)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 316 AA; 34498 MW; D8C521370C54C672 CRC64;
MDLCVTIKGV SFLLQAGLGI LANALVLLAY AHIRLAEARL QPVDAILCHL ALVDLLLLLT
RGVPQTMTVF GMRNLLDDTG CKVVIYTYRI ARALSVCITC MLSVFQAVTV APAAGPLLSG
VKARLPQLLA PTFAALWFIN MAVCIAAPFF SVAPRNGTVP PFTLNLGFCH VDFHDNLSYV
LNGVAVSVRD FAFVGAMLAS SGFILLLLHR HRRQVRAVRR SQGSTMETRA ARTVLMLVIL
YSVFFGIDNV IWIYMLTVAQ VPPVVADMRV FFSSCYASLS PFLIISSNRK LKARMVCATS
EQERQAEDGK NSSGKN
