ORAI2_HUMAN
ID ORAI2_HUMAN Reviewed; 254 AA.
AC Q96SN7; Q6IA68; Q8WY94; Q9H9Y3;
DT 16-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Protein orai-2;
DE AltName: Full=CAP-binding protein complex-interacting protein 2;
DE AltName: Full=Transmembrane protein 142B;
GN Name=ORAI2; Synonyms=C7orf19, CBCIP2, TMEM142B; ORFNames=PP1729;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Teratocarcinoma;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12853948; DOI=10.1038/nature01782;
RA Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H.,
RA Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K.,
RA Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A.,
RA Delehaunty K.D., Miner T.L., Nash W.E., Cordes M., Du H., Sun H.,
RA Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A.,
RA Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P.,
RA Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M.,
RA Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S.,
RA Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R.,
RA Strowmatt C., Latreille P., Miller N., Johnson D., Murray J.,
RA Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W.,
RA Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A.,
RA Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E.,
RA Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E.,
RA Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A.,
RA Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A.,
RA Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R.,
RA McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H.,
RA Wilson R.K.;
RT "The DNA sequence of human chromosome 7.";
RL Nature 424:157-164(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 191-254.
RX PubMed=15498874; DOI=10.1073/pnas.0404089101;
RA Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X.,
RA Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X.,
RA Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S., Gu J.;
RT "Large-scale cDNA transfection screening for genes related to cancer
RT development and progression.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004).
RN [5]
RP PARTIAL NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION.
RX PubMed=16807233; DOI=10.1074/jbc.m604589200;
RA Mercer J.C., Dehaven W.I., Smyth J.T., Wedel B., Boyles R.R., Bird G.S.,
RA Putney J.W. Jr.;
RT "Large store-operated calcium selective currents due to co-expression of
RT Orai1 or Orai2 with the intracellular calcium sensor, Stim1.";
RL J. Biol. Chem. 281:24979-24990(2006).
RN [7]
RP IDENTIFICATION.
RX PubMed=16582901; DOI=10.1038/nature04702;
RA Feske S., Gwack Y., Prakriya M., Srikanth S., Puppel S.-H., Tanasa B.,
RA Hogan P.G., Lewis R.S., Daly M., Rao A.;
RT "A mutation in Orai1 causes immune deficiency by abrogating CRAC channel
RT function.";
RL Nature 441:179-185(2006).
RN [8]
RP FUNCTION.
RX PubMed=17452328; DOI=10.1074/jbc.m611374200;
RA Dehaven W.I., Smyth J.T., Boyles R.R., Putney J.W. Jr.;
RT "Calcium inhibition and calcium potentiation of orai1, orai2, and orai3
RT calcium release-activated calcium channels.";
RL J. Biol. Chem. 282:17548-17556(2007).
RN [9]
RP INTERACTION WITH CRACR2A.
RX PubMed=20418871; DOI=10.1038/ncb2045;
RA Srikanth S., Jung H.J., Kim K.D., Souda P., Whitelegge J., Gwack Y.;
RT "A novel EF-hand protein, CRACR2A, is a cytosolic Ca2+ sensor that
RT stabilizes CRAC channels in T cells.";
RL Nat. Cell Biol. 12:436-446(2010).
CC -!- FUNCTION: Ca(2+) release-activated Ca(2+)-like (CRAC-like) channel
CC subunit which mediates Ca(2+) influx and increase in Ca(2+)-selective
CC current by synergy with the Ca(2+) sensor, STIM1.
CC {ECO:0000269|PubMed:16807233, ECO:0000269|PubMed:17452328}.
CC -!- SUBUNIT: Interacts with CRACR2A/EFCAB4B. {ECO:0000269|PubMed:20418871}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the Orai family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG23755.1; Type=Erroneous translation; Note=Wrong choice of CDS.; Evidence={ECO:0000305};
CC Sequence=AAP22352.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAG33568.1; Type=Erroneous translation; Note=Wrong choice of CDS.; Evidence={ECO:0000305};
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DR EMBL; AK027639; BAB55256.1; -; mRNA.
DR EMBL; AC093668; AAP22351.1; -; Genomic_DNA.
DR EMBL; AC093668; AAP22352.1; ALT_SEQ; Genomic_DNA.
DR EMBL; BC069270; AAH69270.1; -; mRNA.
DR EMBL; AF258552; AAG23755.1; ALT_SEQ; mRNA.
DR EMBL; CR457287; CAG33568.1; ALT_SEQ; mRNA.
DR CCDS; CCDS5722.1; -.
DR RefSeq; NP_001119812.1; NM_001126340.2.
DR RefSeq; NP_001258747.1; NM_001271818.1.
DR RefSeq; NP_116220.1; NM_032831.3.
DR AlphaFoldDB; Q96SN7; -.
DR SMR; Q96SN7; -.
DR BioGRID; 123192; 6.
DR DIP; DIP-48944N; -.
DR IntAct; Q96SN7; 9.
DR STRING; 9606.ENSP00000348752; -.
DR ChEMBL; CHEMBL3038476; -.
DR GuidetoPHARMACOLOGY; 2965; -.
DR iPTMnet; Q96SN7; -.
DR PhosphoSitePlus; Q96SN7; -.
DR BioMuta; ORAI2; -.
DR DMDM; 74732728; -.
DR EPD; Q96SN7; -.
DR jPOST; Q96SN7; -.
DR MassIVE; Q96SN7; -.
DR MaxQB; Q96SN7; -.
DR PaxDb; Q96SN7; -.
DR PeptideAtlas; Q96SN7; -.
DR PRIDE; Q96SN7; -.
DR ProteomicsDB; 78130; -.
DR Antibodypedia; 31061; 165 antibodies from 27 providers.
DR DNASU; 80228; -.
DR Ensembl; ENST00000356387.6; ENSP00000348752.2; ENSG00000160991.17.
DR Ensembl; ENST00000403646.8; ENSP00000385489.3; ENSG00000160991.17.
DR Ensembl; ENST00000473939.2; ENSP00000417928.1; ENSG00000160991.17.
DR Ensembl; ENST00000478730.7; ENSP00000418140.1; ENSG00000160991.17.
DR Ensembl; ENST00000495936.7; ENSP00000420178.2; ENSG00000160991.17.
DR Ensembl; ENST00000498661.6; ENSP00000418464.2; ENSG00000160991.17.
DR Ensembl; ENST00000611770.5; ENSP00000478113.1; ENSG00000160991.17.
DR GeneID; 80228; -.
DR KEGG; hsa:80228; -.
DR MANE-Select; ENST00000495936.7; ENSP00000420178.2; NM_001126340.3; NP_001119812.1.
DR UCSC; uc003uzj.3; human.
DR CTD; 80228; -.
DR DisGeNET; 80228; -.
DR GeneCards; ORAI2; -.
DR HGNC; HGNC:21667; ORAI2.
DR HPA; ENSG00000160991; Low tissue specificity.
DR MIM; 610929; gene.
DR neXtProt; NX_Q96SN7; -.
DR OpenTargets; ENSG00000160991; -.
DR PharmGKB; PA162398454; -.
DR VEuPathDB; HostDB:ENSG00000160991; -.
DR eggNOG; KOG4298; Eukaryota.
DR GeneTree; ENSGT00390000015354; -.
DR InParanoid; Q96SN7; -.
DR OMA; KMSQSGD; -.
DR OrthoDB; 1197243at2759; -.
DR PhylomeDB; Q96SN7; -.
DR TreeFam; TF313576; -.
DR PathwayCommons; Q96SN7; -.
DR Reactome; R-HSA-139853; Elevation of cytosolic Ca2+ levels.
DR Reactome; R-HSA-5578775; Ion homeostasis.
DR Reactome; R-HSA-983695; Antigen activates B Cell Receptor (BCR) leading to generation of second messengers.
DR SignaLink; Q96SN7; -.
DR BioGRID-ORCS; 80228; 13 hits in 1072 CRISPR screens.
DR ChiTaRS; ORAI2; human.
DR GeneWiki; ORAI2; -.
DR GenomeRNAi; 80228; -.
DR Pharos; Q96SN7; Tchem.
DR PRO; PR:Q96SN7; -.
DR Proteomes; UP000005640; Chromosome 7.
DR RNAct; Q96SN7; protein.
DR Bgee; ENSG00000160991; Expressed in renal medulla and 187 other tissues.
DR ExpressionAtlas; Q96SN7; baseline and differential.
DR Genevisible; Q96SN7; HS.
DR GO; GO:0030426; C:growth cone; IEA:Ensembl.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0015279; F:store-operated calcium channel activity; IBA:GO_Central.
DR GO; GO:0002115; P:store-operated calcium entry; IBA:GO_Central.
DR Gene3D; 1.20.140.140; -; 1.
DR InterPro; IPR012446; CRAC_channel.
DR InterPro; IPR030034; Orai-2.
DR InterPro; IPR038350; Orai_sf.
DR PANTHER; PTHR31501; PTHR31501; 1.
DR PANTHER; PTHR31501:SF5; PTHR31501:SF5; 1.
DR Pfam; PF07856; Orai-1; 1.
PE 1: Evidence at protein level;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..254
FT /note="Protein orai-2"
FT /id="PRO_0000234391"
FT TRANSMEM 66..83
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 94..114
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 148..168
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 196..216
FT /note="Helical"
FT /evidence="ECO:0000255"
FT VARIANT 15
FT /note="A -> P (in dbSNP:rs34947403)"
FT /id="VAR_053543"
SQ SEQUENCE 254 AA; 28570 MW; D53CD2376F48E23C CRC64;
MSAELNVPID PSAPACPEPG HKGMDYRDWV RRSYLELVTS NHHSVQALSW RKLYLSRAKL
KASSRTSALL SGFAMVAMVE VQLETQYQYP RPLLIAFSAC TTVLVAVHLF ALLISTCILP
NVEAVSNIHN LNSISESPHE RMHPYIELAW GFSTVLGILL FLAEVVLLCW IKFLPVDARR
QPGPPPGPGS HTGWQAALVS TIIMVPVGLI FVVFTIHFYR SLVRHKTERH NREIEELHKL
KVQLDGHERS LQVL
