ORAI3_HUMAN
ID ORAI3_HUMAN Reviewed; 295 AA.
AC Q9BRQ5; Q96BI8;
DT 16-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 2.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Protein orai-3;
DE AltName: Full=Transmembrane protein 142C;
GN Name=ORAI3; Synonyms=TMEM142C;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Lung, and Uterus;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP IDENTIFICATION.
RX PubMed=16582901; DOI=10.1038/nature04702;
RA Feske S., Gwack Y., Prakriya M., Srikanth S., Puppel S.-H., Tanasa B.,
RA Hogan P.G., Lewis R.S., Daly M., Rao A.;
RT "A mutation in Orai1 causes immune deficiency by abrogating CRAC channel
RT function.";
RL Nature 441:179-185(2006).
RN [3]
RP INTERACTION WITH CRACR2A.
RX PubMed=20418871; DOI=10.1038/ncb2045;
RA Srikanth S., Jung H.J., Kim K.D., Souda P., Whitelegge J., Gwack Y.;
RT "A novel EF-hand protein, CRACR2A, is a cytosolic Ca2+ sensor that
RT stabilizes CRAC channels in T cells.";
RL Nat. Cell Biol. 12:436-446(2010).
RN [4]
RP FUNCTION, TISSUE SPECIFICITY, AND MUTAGENESIS OF GLY-170.
RX PubMed=20354224; DOI=10.1126/scisignal.2000672;
RA Bogeski I., Kummerow C., Al-Ansary D., Schwarz E.C., Koehler R., Kozai D.,
RA Takahashi N., Peinelt C., Griesemer D., Bozem M., Mori Y., Hoth M.,
RA Niemeyer B.A.;
RT "Differential redox regulation of ORAI ion channels: a mechanism to tune
RT cellular calcium signaling.";
RL Sci. Signal. 3:RA24-RA24(2010).
CC -!- FUNCTION: Ca(2+) release-activated Ca(2+)-like (CRAC-like) channel
CC subunit which mediates Ca(2+) influx and increase in Ca(2+)-selective
CC current by synergy with the Ca(2+) sensor, STIM1.
CC {ECO:0000269|PubMed:20354224}.
CC -!- SUBUNIT: Interacts with CRACR2A/EFCAB4B. {ECO:0000269|PubMed:20418871}.
CC -!- INTERACTION:
CC Q9BRQ5; Q96D31: ORAI1; NbExp=4; IntAct=EBI-4279484, EBI-2291476;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed in both naive and effector T helper cells
CC with higher levels in effector cells. {ECO:0000269|PubMed:20354224}.
CC -!- SIMILARITY: Belongs to the Orai family. {ECO:0000305}.
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DR EMBL; BC006126; AAH06126.2; -; mRNA.
DR EMBL; BC015555; AAH15555.1; -; mRNA.
DR EMBL; BC022786; AAH22786.1; -; mRNA.
DR CCDS; CCDS10697.1; -.
DR RefSeq; NP_689501.1; NM_152288.2.
DR AlphaFoldDB; Q9BRQ5; -.
DR SMR; Q9BRQ5; -.
DR DIP; DIP-48945N; -.
DR IntAct; Q9BRQ5; 4.
DR STRING; 9606.ENSP00000322249; -.
DR ChEMBL; CHEMBL3038476; -.
DR TCDB; 1.A.52.1.2; the ca(2+) release-activated ca(2+) (crac) channel (crac-c) family.
DR GlyGen; Q9BRQ5; 2 sites, 1 O-linked glycan (2 sites).
DR iPTMnet; Q9BRQ5; -.
DR PhosphoSitePlus; Q9BRQ5; -.
DR BioMuta; ORAI3; -.
DR DMDM; 74732916; -.
DR jPOST; Q9BRQ5; -.
DR MassIVE; Q9BRQ5; -.
DR PaxDb; Q9BRQ5; -.
DR PeptideAtlas; Q9BRQ5; -.
DR PRIDE; Q9BRQ5; -.
DR ProteomicsDB; 78806; -.
DR Antibodypedia; 3108; 270 antibodies from 29 providers.
DR DNASU; 93129; -.
DR Ensembl; ENST00000318663.5; ENSP00000322249.4; ENSG00000175938.7.
DR GeneID; 93129; -.
DR KEGG; hsa:93129; -.
DR MANE-Select; ENST00000318663.5; ENSP00000322249.4; NM_152288.3; NP_689501.1.
DR UCSC; uc002eac.4; human.
DR CTD; 93129; -.
DR DisGeNET; 93129; -.
DR GeneCards; ORAI3; -.
DR HGNC; HGNC:28185; ORAI3.
DR HPA; ENSG00000175938; Low tissue specificity.
DR MIM; 610930; gene.
DR neXtProt; NX_Q9BRQ5; -.
DR OpenTargets; ENSG00000175938; -.
DR PharmGKB; PA162398465; -.
DR VEuPathDB; HostDB:ENSG00000175938; -.
DR eggNOG; KOG4298; Eukaryota.
DR GeneTree; ENSGT00390000015354; -.
DR HOGENOM; CLU_062509_1_1_1; -.
DR InParanoid; Q9BRQ5; -.
DR OMA; MASTVIM; -.
DR OrthoDB; 1197243at2759; -.
DR PhylomeDB; Q9BRQ5; -.
DR TreeFam; TF313576; -.
DR PathwayCommons; Q9BRQ5; -.
DR SignaLink; Q9BRQ5; -.
DR BioGRID-ORCS; 93129; 19 hits in 1075 CRISPR screens.
DR ChiTaRS; ORAI3; human.
DR GenomeRNAi; 93129; -.
DR Pharos; Q9BRQ5; Tbio.
DR PRO; PR:Q9BRQ5; -.
DR Proteomes; UP000005640; Chromosome 16.
DR RNAct; Q9BRQ5; protein.
DR Bgee; ENSG00000175938; Expressed in right lobe of liver and 96 other tissues.
DR ExpressionAtlas; Q9BRQ5; baseline and differential.
DR Genevisible; Q9BRQ5; HS.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015279; F:store-operated calcium channel activity; IBA:GO_Central.
DR GO; GO:0002115; P:store-operated calcium entry; IBA:GO_Central.
DR Gene3D; 1.20.140.140; -; 1.
DR InterPro; IPR012446; CRAC_channel.
DR InterPro; IPR030032; Orai-3.
DR InterPro; IPR038350; Orai_sf.
DR PANTHER; PTHR31501; PTHR31501; 1.
DR PANTHER; PTHR31501:SF6; PTHR31501:SF6; 1.
DR Pfam; PF07856; Orai-1; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Phosphoprotein; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..295
FT /note="Protein orai-3"
FT /id="PRO_0000234395"
FT TRANSMEM 63..82
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 95..115
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 157..177
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 244..264
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..23
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 204..238
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 204..220
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 42
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q6P8G8"
FT MOD_RES 45
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q6P8G8"
FT MUTAGEN 170
FT /note="G->C: Causes redox sensitivity with channel activity
FT inhibited by hydrogen peroxide."
FT /evidence="ECO:0000269|PubMed:20354224"
SQ SEQUENCE 295 AA; 31499 MW; DC44C8AE7EEF238D CRC64;
MKGGEGDAGE QAPLNPEGES PAGSATYREF VHRGYLDLMG ASQHSLRALS WRRLYLSRAK
LKASSRTSAL LSGFAMVAMV EVQLESDHEY PPGLLVAFSA CTTVLVAVHL FALMVSTCLL
PHIEAVSNIH NLNSVHQSPH QRLHRYVELA WGFSTALGTF LFLAEVVLVG WVKFVPIGAP
LDTPTPMVPT SRVPGTLAPV ATSLSPASNL PRSSASAAPS QAEPACPPRQ ACGGGGAHGP
GWQAAMASTA IMVPVGLVFV AFALHFYRSL VAHKTDRYKQ ELEELNRLQG ELQAV
