ORANG_BRAOB
ID ORANG_BRAOB Reviewed; 305 AA.
AC A2T1U1;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 06-MAR-2007, sequence version 1.
DT 25-MAY-2022, entry version 29.
DE RecName: Full=Protein ORANGE, chloroplastic {ECO:0000305};
DE Short=BoOr {ECO:0000305};
DE AltName: Full=DnaJ-like cysteine-rich domain-containing protein Or {ECO:0000303|PubMed:17172359};
DE Flags: Precursor;
GN Name=OR {ECO:0000303|PubMed:17172359};
OS Brassica oleracea var. botrytis (Cauliflower).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX NCBI_TaxID=3715;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, AND POLYMORPHISM.
RX PubMed=17172359; DOI=10.1105/tpc.106.046417;
RA Lu S., Van Eck J., Zhou X., Lopez A.B., O'Halloran D.M., Cosman K.M.,
RA Conlin B.J., Paolillo D.J., Garvin D.F., Vrebalov J., Kochian L.V.,
RA Kupper H., Earle E.D., Cao J., Li L.;
RT "The cauliflower Or gene encodes a DnaJ cysteine-rich domain-containing
RT protein that mediates high levels of beta-carotene accumulation.";
RL Plant Cell 18:3594-3605(2006).
RN [2]
RP FUNCTION.
RX PubMed=18256051; DOI=10.1093/jxb/erm299;
RA Lopez A.B., Van Eck J., Conlin B.J., Paolillo D.J., O'Neill J., Li L.;
RT "Effect of the cauliflower Or transgene on carotenoid accumulation and
RT chromoplast formation in transgenic potato tubers.";
RL J. Exp. Bot. 59:213-223(2008).
RN [3]
RP FUNCTION, INTERACTION WITH ERF1-2, AND SUBCELLULAR LOCATION.
RX PubMed=21175633; DOI=10.1111/j.1469-8137.2010.03578.x;
RA Zhou X., Sun T.H., Wang N., Ling H.Q., Lu S., Li L.;
RT "The cauliflower Orange gene enhances petiole elongation by suppressing
RT expression of eukaryotic release factor 1.";
RL New Phytol. 190:89-100(2011).
RN [4]
RP FUNCTION.
RX PubMed=23887833; DOI=10.1007/s10265-013-0579-x;
RA Men X., Dong K.;
RT "Or mutation leads to photo-oxidative stress responses in cauliflower
RT (Brassica oleracea) seedlings during de-etiolation.";
RL J. Plant Res. 126:823-832(2013).
CC -!- FUNCTION: Involved in chromoplast differentiation. Is associated with a
CC cellular process that triggers the differentiation of pro-plastids or
CC other non-colored plastids into chromoplasts for carotenoid
CC accumulation (PubMed:17172359, PubMed:18256051). Associated with
CC carotenoid accumulation in de-etiolated cotyledons (PubMed:23887833).
CC Controls leaf petiole elongation by suppressing the expression of ERF1
CC genes (PubMed:21175633). {ECO:0000269|PubMed:17172359,
CC ECO:0000269|PubMed:18256051, ECO:0000269|PubMed:21175633,
CC ECO:0000269|PubMed:23887833}.
CC -!- SUBUNIT: Interacts with ERF1-2. {ECO:0000269|PubMed:21175633}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane {ECO:0000305}.
CC Plastid {ECO:0000269|PubMed:17172359}. Nucleus
CC {ECO:0000269|PubMed:21175633}. Note=Targeted to non-green plastids.
CC {ECO:0000269|PubMed:17172359}.
CC -!- TISSUE SPECIFICITY: Expressed in young leaves, curds and flower buds.
CC {ECO:0000269|PubMed:17172359}.
CC -!- POLYMORPHISM: A probable gain-of-function mutant of OR gene confers the
CC accumulation of high levels of beta-carotene in various tissues
CC normally devoid of carotenoids, converting the white color of curd
CC tissue into the distinct orange color. Hybrid OR cauliflower containing
CC large amounts of beta-carotene is commercially available.
CC {ECO:0000305|PubMed:17172359}.
CC -!- MISCELLANEOUS: Its sequence is related to the DnaJ family but lacks the
CC J domain. The CR-type-like region is similar to CR-type zinc-fingers.
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the orange-like family.
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DR EMBL; DQ482456; ABH07405.1; -; mRNA.
DR AlphaFoldDB; A2T1U1; -.
DR GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0009661; P:chromoplast organization; IMP:UniProtKB.
DR GO; GO:1904143; P:positive regulation of carotenoid biosynthetic process; IMP:UniProtKB.
DR GO; GO:0009826; P:unidimensional cell growth; IMP:UniProtKB.
PE 1: Evidence at protein level;
KW Chloroplast; Membrane; Nucleus; Plastid; Repeat; Transit peptide;
KW Transmembrane; Transmembrane helix.
FT TRANSIT 1..54
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 55..305
FT /note="Protein ORANGE, chloroplastic"
FT /id="PRO_0000438014"
FT TRANSMEM 144..164
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 197..217
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REPEAT 228..235
FT /note="CXXCXGXG motif"
FT /evidence="ECO:0000305"
FT REPEAT 239..246
FT /note="CXXCXXXG motif"
FT /evidence="ECO:0000305"
FT REPEAT 272..279
FT /note="CXXCXGXG motif"
FT /evidence="ECO:0000305"
FT REPEAT 283..290
FT /note="CXXCXXXG motif"
FT /evidence="ECO:0000305"
FT REGION 206..297
FT /note="CR-type-like"
FT /evidence="ECO:0000305"
SQ SEQUENCE 305 AA; 33403 MW; FED4B38112E91BB4 CRC64;
MSCLGRILSV SYPPDPYGSR LSVSKLSSPG RNRRLRWRFT ALDSDSSSLD SDSSDKFAAG
FCIIEGPETV QDFAKMQLQE IQDNIRSRRN KIFLHMEEVR RLRIQQRIRN TELGIIDEEQ
EHELPNFPSF IPFLPPLTAA NLRVYYATCF SLIAGIILFG GLLAPTLELK LGIGGTSYKD
FIQSLHLPMQ LSQVDPIVAS FSGGAVGVIS ALMVVEVNNV KQQEHKRCKY CLGTGYLACA
RCSSTGSLII SEPVSAIAGG NHSVSTSKTE RCSNCSGAGK VMCPTCLCTG MAMASEHDPR
IDPFL
