ORC1_DICDI
ID ORC1_DICDI Reviewed; 631 AA.
AC Q54RM2;
DT 29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=Origin recognition complex subunit 1;
DE AltName: Full=Origin replication complex subunit A;
GN Name=orcA; Synonyms=orc1; ORFNames=DDB_G0283073;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Component of the origin recognition complex (ORC) that binds
CC origins of replication. DNA-binding is ATP-dependent, however specific
CC DNA sequences that define origins of replication have not been
CC identified so far. ORC is required to assemble the pre-replication
CC complex necessary to initiate DNA replication (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: ORC is composed of six subunits. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the ORC1 family. {ECO:0000305}.
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DR EMBL; AAFI02000049; EAL65980.1; -; Genomic_DNA.
DR RefSeq; XP_639329.1; XM_634237.1.
DR AlphaFoldDB; Q54RM2; -.
DR SMR; Q54RM2; -.
DR STRING; 44689.DDB0233110; -.
DR PaxDb; Q54RM2; -.
DR EnsemblProtists; EAL65980; EAL65980; DDB_G0283073.
DR GeneID; 8623900; -.
DR KEGG; ddi:DDB_G0283073; -.
DR dictyBase; DDB_G0283073; orcA.
DR eggNOG; KOG1514; Eukaryota.
DR HOGENOM; CLU_433777_0_0_1; -.
DR InParanoid; Q54RM2; -.
DR OMA; RSEVAFK; -.
DR PhylomeDB; Q54RM2; -.
DR Reactome; R-DDI-68616; Assembly of the ORC complex at the origin of replication.
DR Reactome; R-DDI-68689; CDC6 association with the ORC:origin complex.
DR Reactome; R-DDI-68949; Orc1 removal from chromatin.
DR Reactome; R-DDI-68962; Activation of the pre-replicative complex.
DR PRO; PR:Q54RM2; -.
DR Proteomes; UP000002195; Chromosome 4.
DR GO; GO:0005664; C:nuclear origin of replication recognition complex; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; ISS:dictyBase.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0003688; F:DNA replication origin binding; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006270; P:DNA replication initiation; IBA:GO_Central.
DR GO; GO:0033314; P:mitotic DNA replication checkpoint signaling; IBA:GO_Central.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR041083; AAA_lid_10.
DR InterPro; IPR003959; ATPase_AAA_core.
DR InterPro; IPR020793; ORC1.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR10763:SF23; PTHR10763:SF23; 1.
DR Pfam; PF00004; AAA; 1.
DR Pfam; PF17872; AAA_lid_10; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 3: Inferred from homology;
KW ATP-binding; DNA replication; DNA-binding; Magnesium; Metal-binding;
KW Nucleotide-binding; Nucleus; Reference proteome.
FT CHAIN 1..631
FT /note="Origin recognition complex subunit 1"
FT /id="PRO_0000329974"
FT REGION 1..164
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 20..50
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 51..79
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 88..107
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 108..129
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 141..164
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 230
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250|UniProtKB:Q13415"
FT BINDING 265..273
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250|UniProtKB:Q13415"
FT BINDING 361
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250|UniProtKB:Q13415"
FT BINDING 362
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250|UniProtKB:Q13415"
FT BINDING 362
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250|UniProtKB:Q13415"
FT BINDING 395
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250|UniProtKB:Q13415"
FT BINDING 460
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250|UniProtKB:Q13415"
SQ SEQUENCE 631 AA; 72401 MW; 7A59F1C555ABCA8C CRC64;
MTDESSSSIS YPPIKKYKKS LKLNNNTNNN NKNNNNHNNR DIVMQPNSTD EESSDNEKIG
FSDPENEKIN KHKASFKDSN DENNNNSTYY EDTDDDDYED EDEDENHKIK DESDNSEDFN
NHTKNTTDLD EGFKSTVNGS ESEEEEEEEE YEEEEEEDEE GKFNLDQYDE FYEDDDDGDD
DDDDDENAQD KVEILQIEHQ NLDNDNDNDN DYANDNIYTK AKEALHLSAV PDKLPGREKE
KATIASFIRA KLKANESGGC LYIAGMPGTG KTATVKEIIK ELQAKKKQQG GGGGLNFQFI
EINGMQLSDP HQLYHILYNK MQKTRKSLEP KKISSQDALR LIQRNFELKN KKKQFRVILV
DEFDSLITKK QTVIYNLFEW PNKPNSKLII IAIANTMNLP DTLLPRVKSR MGLQKVPFTP
YNIEQLETII KYRLQDLDAF DEESIQICSK RVAAVCGDAR RALEICRKAA TIANQEYQKK
LLIFNNSNNN KSLSGSQKLP IPGPITADHI EEVFEQFSSP LLKKLNQLSF YEKLFLLCIC
RENQFSNVPE VKYGTISTRM RIITKKINVS CPNPTQLFQL AGNLLGCKFI IIQDDKPINW
DHQIKLNLPL EDLSFGLEQD PDLKNLEISN N
