ORC2_ARATH
ID ORC2_ARATH Reviewed; 363 AA.
AC Q38899;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 25-MAY-2022, entry version 129.
DE RecName: Full=Origin of replication complex subunit 2 {ECO:0000303|PubMed:16179646};
DE Short=AtORC2 {ECO:0000303|PubMed:16179646};
GN Name=ORC2 {ECO:0000303|PubMed:16179646};
GN OrderedLocusNames=At2g37560 {ECO:0000312|Araport:AT2G37560};
GN ORFNames=F13M22.6 {ECO:0000312|EMBL:AAC23627.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=7502077; DOI=10.1126/science.270.5242.1667;
RA Gavin K.A., Hidaka M., Stillman B.D.;
RT "Conserved initiator proteins in eukaryotes.";
RL Science 270:1667-1671(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP SUBUNIT, INTERACTION WITH ORC3 AND ORC4, TISSUE SPECIFICITY, INDUCTION BY
RP SUCROSE, AND GENE FAMILY.
RC STRAIN=cv. Columbia;
RX PubMed=15358564; DOI=10.1016/j.febslet.2004.07.088;
RA Masuda H.P., Ramos G.B.A., de Almeida-Engler J., Cabral L.M.,
RA Coqueiro V.M., Macrini C.M.T., Ferreira P.C.G., Hemerly A.S.;
RT "Genome based identification and analysis of the pre-replicative complex of
RT Arabidopsis thaliana.";
RL FEBS Lett. 574:192-202(2004).
RN [7]
RP FUNCTION, DISRUPTION PHENOTYPE, AND DEVELOPMENTAL STAGE.
RX PubMed=15020747; DOI=10.1105/tpc.019059;
RA Collinge M.A., Spillane C., Kohler C., Gheyselinck J., Grossniklaus U.;
RT "Genetic interaction of an origin recognition complex subunit and the
RT Polycomb group gene MEDEA during seed development.";
RL Plant Cell 16:1035-1046(2004).
RN [8]
RP SUBUNIT, INTERACTION WITH ORC1A; ORC1B; ORC3; ORC4; ORC5 AND ORC6, TISSUE
RP SPECIFICITY, INDUCTION BY E2F AND SUCROSE, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16179646; DOI=10.1093/nar/gki854;
RA Diaz-Trivino S., Castellano M.M., Sanchez M.P., Ramirez-Parra E.,
RA Desvoyes B., Gutierrez C.;
RT "The genes encoding Arabidopsis ORC subunits are E2F targets and the two
RT ORC1 genes are differently expressed in proliferating and endoreplicating
RT cells.";
RL Nucleic Acids Res. 33:5404-5414(2005).
RN [9]
RP REVIEW ON THE CORE DNA REPLICATION MACHINERY.
RX PubMed=17556508; DOI=10.1104/pp.107.101105;
RA Shultz R.W., Tatineni V.M., Hanley-Bowdoin L., Thompson W.F.;
RT "Genome-wide analysis of the core DNA replication machinery in the higher
RT plants Arabidopsis and rice.";
RL Plant Physiol. 144:1697-1714(2007).
CC -!- FUNCTION: Essential protein (PubMed:15020747). Component of the origin
CC recognition complex (ORC) that binds origins of replication. DNA-
CC binding is ATP-dependent, however specific DNA sequences that define
CC origins of replication have not been identified so far. ORC is required
CC to assemble the pre-replication complex necessary to initiate DNA
CC replication (By similarity). {ECO:0000250,
CC ECO:0000269|PubMed:15020747}.
CC -!- SUBUNIT: Component of the origin recognition complex (ORC) composed of
CC at least ORC1 (ORC1A or ORC1B), ORC2, ORC3, ORC4, ORC5 and ORC6. ORC is
CC regulated in a cell-cycle and development dependent manner. It is
CC sequentially assembled at the exit from anaphase of mitosis and
CC disassembled as cells enter S phase. Interacts directly with ORC1A,
CC ORC1B, ORC3, ORC4, ORC5 and ORC6. {ECO:0000269|PubMed:15358564,
CC ECO:0000269|PubMed:16179646}.
CC -!- INTERACTION:
CC Q38899; Q6EWX1: ORC4; NbExp=2; IntAct=EBI-2114089, EBI-2114176;
CC Q38899; Q6EWX0: ORC5; NbExp=2; IntAct=EBI-2114089, EBI-2114109;
CC Q38899; Q9ZVH3: ORC6; NbExp=2; IntAct=EBI-2114089, EBI-2114077;
CC Q38899; Q9LF53: RGL3; NbExp=3; IntAct=EBI-2114089, EBI-15681313;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q38899-1; Sequence=Displayed;
CC -!- TISSUE SPECIFICITY: Expressed in female gametophyte and embryos, and,
CC at low levels, in trichomes, pollen and the mesophyll of leaves. Follow
CC a cell-cycle regulation with a peak at the G1/S-phase
CC (PubMed:16179646). Mostly expressed in siliques, flowers, flower buds
CC and leaves, and, to a lower exent, in roots and stems (PubMed:16179646,
CC PubMed:15358564). {ECO:0000269|PubMed:15020747,
CC ECO:0000269|PubMed:15358564, ECO:0000269|PubMed:16179646}.
CC -!- DEVELOPMENTAL STAGE: Detected in the mature female gametophyte, in the
CC synergids and central cell. Mostly expressed in embryos (but not the
CC suspensor) and endosperm of preglobular stage seeds. Present in pollen
CC as well as in pollen tubes. {ECO:0000269|PubMed:15020747}.
CC -!- INDUCTION: Regulated by E2F (PubMed:16179646). Accumulates rapidly
CC after cell cycle reactivation by sucrose addition following cell cycle
CC arrest mediated by sucrose deprivation (PubMed:16179646,
CC PubMed:15358564). {ECO:0000269|PubMed:15358564,
CC ECO:0000269|PubMed:16179646}.
CC -!- DISRUPTION PHENOTYPE: Zygotic lethal mutant phenotype. Seeds abort
CC early, with embryos made of up to eight cells, characterized by
CC enlarged nuclei in endosperm with arrested cell cycle. These phenotypes
CC are partly suppressed by mutation in MEA/MEDEA.
CC {ECO:0000269|PubMed:15020747}.
CC -!- SIMILARITY: Belongs to the ORC2 family. {ECO:0000305}.
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DR EMBL; U40269; AAC49131.1; -; mRNA.
DR EMBL; AC004684; AAC23627.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC09416.1; -; Genomic_DNA.
DR EMBL; AY070386; AAL49882.1; -; mRNA.
DR EMBL; AY096598; AAM20248.1; -; mRNA.
DR EMBL; AY088188; AAM65731.1; -; mRNA.
DR PIR; T02522; T02522.
DR RefSeq; NP_181292.1; NM_129311.3. [Q38899-1]
DR AlphaFoldDB; Q38899; -.
DR SMR; Q38899; -.
DR BioGRID; 3676; 2.
DR IntAct; Q38899; 7.
DR STRING; 3702.AT2G37560.1; -.
DR PaxDb; Q38899; -.
DR ProteomicsDB; 248905; -. [Q38899-1]
DR DNASU; 818332; -.
DR EnsemblPlants; AT2G37560.1; AT2G37560.1; AT2G37560. [Q38899-1]
DR GeneID; 818332; -.
DR Gramene; AT2G37560.1; AT2G37560.1; AT2G37560. [Q38899-1]
DR KEGG; ath:AT2G37560; -.
DR Araport; AT2G37560; -.
DR TAIR; locus:2040706; AT2G37560.
DR eggNOG; KOG2928; Eukaryota.
DR InParanoid; Q38899; -.
DR OMA; YDFELAY; -.
DR PhylomeDB; Q38899; -.
DR PRO; PR:Q38899; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q38899; baseline and differential.
DR Genevisible; Q38899; AT.
DR GO; GO:0005664; C:nuclear origin of replication recognition complex; IBA:GO_Central.
DR GO; GO:0000808; C:origin recognition complex; ISS:TAIR.
DR GO; GO:0003688; F:DNA replication origin binding; ISS:TAIR.
DR GO; GO:0006260; P:DNA replication; ISS:TAIR.
DR InterPro; IPR007220; ORC2.
DR PANTHER; PTHR14052; PTHR14052; 1.
DR Pfam; PF04084; ORC2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; DNA replication; Nucleus; Reference proteome.
FT CHAIN 1..363
FT /note="Origin of replication complex subunit 2"
FT /id="PRO_0000127080"
SQ SEQUENCE 363 AA; 40811 MW; 773A02B3F8FE25DF CRC64;
MEDIENIEED EYGFSRNYFL AKELGGASKR SAHKLSDIHI VDEQELRETA STIEMKHSKE
ISELMSDYKT MYSKWVFELR CGFGLLMYGF GSKKALVEDF ASASLTDYSV VVINGYLPSV
NLKQVLLALA ELLSELLKCK RKSSGSLSKG QETFPSRSMD DILSFLHGPQ SGDKDCFICV
VVHNIDGPAL RDPESQQTLA RLSSCSHIRL VASIDHVNAP LLWDKKMVHK QFNWLWHHVP
TFAPYNVEGV FFPLVLAQGS TAQTAKTAAI VLQSLTPNGQ NVFKILAEYQ LSHPDEDGMP
TDDLYSASRE RFFVSSQVTL NSHLTEFKDH ELVKTKRNSD GQECLNIPLT SDAIRQLLLD
LNQ
