ID   ORC2_BOVIN              Reviewed;         577 AA.
AC   A6QNM3;
DT   29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   29-APR-2008, sequence version 2.
DT   03-AUG-2022, entry version 62.
DE   RecName: Full=Origin recognition complex subunit 2;
GN   Name=ORC2; Synonyms=ORC2L;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Thymus;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Component of the origin recognition complex (ORC) that binds
CC       origins of replication. DNA-binding is ATP-dependent. The specific DNA
CC       sequences that define origins of replication have not been identified
CC       yet. ORC is required to assemble the pre-replication complex necessary
CC       to initiate DNA replication. Binds histone H3 and H4 trimethylation
CC       marks H3K9me3, H3K20me3 and H4K27me3. Stabilizes LRWD1, by protecting
CC       it from ubiquitin-mediated proteasomal degradation. Also stabilizes
CC       ORC3 (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of ORC, a complex composed of at least 6 subunits:
CC       ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6. ORC is regulated in a cell-cycle
CC       dependent manner. It is sequentially assembled at the exit from
CC       anaphase of mitosis and disassembled as cells enter S phase (By
CC       similarity). Interacts with DBF4 (By similarity). Interacts with MCM10.
CC       Interacts with LRWD1 throughout the cell cycle; this interaction, wich
CC       occurs only with non-ubiquitinated form of LRWD1, prevents LRWD1
CC       ubiquitination and hence stabilizes the protein. Interacts with POLQ
CC       (By similarity). {ECO:0000250|UniProtKB:Q13416,
CC       ECO:0000250|UniProtKB:Q60862}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ORC2 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAI48897.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; BC148896; AAI48897.1; ALT_INIT; mRNA.
DR   RefSeq; NP_001095412.1; NM_001101942.2.
DR   AlphaFoldDB; A6QNM3; -.
DR   SMR; A6QNM3; -.
DR   STRING; 9913.ENSBTAP00000042074; -.
DR   PaxDb; A6QNM3; -.
DR   PRIDE; A6QNM3; -.
DR   GeneID; 511281; -.
DR   KEGG; bta:511281; -.
DR   CTD; 4999; -.
DR   eggNOG; KOG2928; Eukaryota.
DR   InParanoid; A6QNM3; -.
DR   Proteomes; UP000009136; Unplaced.
DR   GO; GO:0005664; C:nuclear origin of replication recognition complex; ISS:UniProtKB.
DR   GO; GO:0003688; F:DNA replication origin binding; IBA:GO_Central.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR   InterPro; IPR007220; ORC2.
DR   PANTHER; PTHR14052; PTHR14052; 1.
DR   Pfam; PF04084; ORC2; 1.
PE   2: Evidence at transcript level;
KW   DNA replication; Nucleus; Phosphoprotein; Reference proteome.
FT   CHAIN           1..577
FT                   /note="Origin recognition complex subunit 2"
FT                   /id="PRO_0000329972"
FT   REPEAT          1..100
FT                   /note="Involved in LRWD1-binding"
FT                   /evidence="ECO:0000250"
FT   REGION          83..103
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          133..204
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        185..203
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         117
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000250|UniProtKB:Q13416"
FT   MOD_RES         227
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000250|UniProtKB:Q13416"
FT   MOD_RES         249
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q13416"
FT   MOD_RES         281
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q13416"
SQ   SEQUENCE   577 AA;  66263 MW;  C1750C5873386A7D CRC64;
     MTSTLELKAD KMLKVQFVGD DDVLNHILDR EGGPKIKKER AQLLVNTQKI IKKPENELEE
     DDQEVLKDHN YVEVLGQDVQ ESLKNGSATD GGNKVYPFQN RKHPEKMAKL ASELAKTPRK
     SVLLNMKNDP KITIDIPQCS KGHSSSDKSQ LKNNDKSEFL STAPRGLRKR LIVPRSHSDS
     ESEYSASNSE DDEGVAQEYE EDTNEDILSQ KIQAQNREVS APVFRETPSK KMKREKTSHL
     VEKYFEAHSS SKVLTSDRTL QKLKRAKLDQ QTLHNLLSKV SPSFSAELKQ LNQQHEQLFH
     KWMLQLHLGF NIVLYGLGSK RDLLERFRTT MLQDCIHIVI NGFFPGISVK SVLNSVTEVL
     DHVGTFRSVL DQLEWITNKF KEDSSLELFL LIHNLDSQML RGDKSQQIIG QLSSLRNIYL
     IASIDHLNAP LMWDHAKQSL YNWLWYETTT YSHYTEETSY ENSLLVKQSG SLPLSSLIHV
     LRSLTPNARG IFRLLIKYQL DNQDNPSYIG LCFQDFYQQC REAFLVNSDL TLRAQLTEFR
     DHKLIRTKKG TDGVEYLLIP VDNGILTDFL ENEEEEL