ORC2_MOUSE
ID ORC2_MOUSE Reviewed; 576 AA.
AC Q60862;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Origin recognition complex subunit 2;
GN Name=Orc2; Synonyms=Orc2l;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=8808289; DOI=10.1006/geno.1996.0018;
RA Takahara K., Bong M., Brevard R., Eddy R.L., Haley L.L., Sait S.J.,
RA Shows T.B., Hoffman G.G., Greenspan D.S.;
RT "Mouse and human homologues of the yeast origin of replication recognition
RT complex subunit ORC2 and chromosomal localization of the cognate human gene
RT ORC2L.";
RL Genomics 31:119-122(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Kidney;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP INTERACTION WITH DBF4.
RX PubMed=12614612; DOI=10.1016/s0022-2836(03)00079-2;
RA Kneissl M., Puetter V., Szalay A.A., Grummt F.;
RT "Interaction and assembly of murine pre-replicative complex proteins in
RT yeast and mouse cells.";
RL J. Mol. Biol. 327:111-128(2003).
CC -!- FUNCTION: Component of the origin recognition complex (ORC) that binds
CC origins of replication. DNA-binding is ATP-dependent. The specific DNA
CC sequences that define origins of replication have not been identified
CC yet. ORC is required to assemble the pre-replication complex necessary
CC to initiate DNA replication (By similarity). Binds histone H3 and H4
CC trimethylation marks H3K9me3, H3K20me3 and H4K27me3. Stabilizes LRWD1,
CC by protecting it from ubiquitin-mediated proteasomal degradation. Also
CC stabilizes ORC3 (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of ORC, a complex composed of at least 6 subunits:
CC ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6. ORC is regulated in a cell-cycle
CC dependent manner. It is sequentially assembled at the exit from
CC anaphase of mitosis and disassembled as cells enter S phase (By
CC similarity). Interacts with DBF4 (PubMed:12614612). Interacts with
CC MCM10. Interacts with LRWD1 throughout the cell cycle; this
CC interaction, wich occurs only with non-ubiquitinated form of LRWD1,
CC prevents LRWD1 ubiquitination and hence stabilizes the protein.
CC Interacts with POLQ (By similarity). {ECO:0000250|UniProtKB:Q13416,
CC ECO:0000269|PubMed:12614612}.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- SIMILARITY: Belongs to the ORC2 family. {ECO:0000305}.
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DR EMBL; U27457; AAB33994.1; -; mRNA.
DR EMBL; BC015257; AAH15257.1; -; mRNA.
DR CCDS; CCDS14975.1; -.
DR RefSeq; NP_001258455.1; NM_001271526.1.
DR RefSeq; NP_032791.1; NM_008765.3.
DR AlphaFoldDB; Q60862; -.
DR SMR; Q60862; -.
DR BioGRID; 201975; 11.
DR ComplexPortal; CPX-1915; Nuclear origin of replication recognition complex.
DR CORUM; Q60862; -.
DR IntAct; Q60862; 10.
DR STRING; 10090.ENSMUSP00000027198; -.
DR iPTMnet; Q60862; -.
DR PhosphoSitePlus; Q60862; -.
DR EPD; Q60862; -.
DR MaxQB; Q60862; -.
DR PaxDb; Q60862; -.
DR PRIDE; Q60862; -.
DR ProteomicsDB; 294106; -.
DR Antibodypedia; 34131; 226 antibodies from 37 providers.
DR DNASU; 18393; -.
DR Ensembl; ENSMUST00000027198; ENSMUSP00000027198; ENSMUSG00000026037.
DR GeneID; 18393; -.
DR KEGG; mmu:18393; -.
DR UCSC; uc007bcd.2; mouse.
DR CTD; 4999; -.
DR MGI; MGI:1328306; Orc2.
DR VEuPathDB; HostDB:ENSMUSG00000026037; -.
DR eggNOG; KOG2928; Eukaryota.
DR GeneTree; ENSGT00390000015228; -.
DR InParanoid; Q60862; -.
DR OMA; FPQWAFE; -.
DR OrthoDB; 1404162at2759; -.
DR PhylomeDB; Q60862; -.
DR TreeFam; TF101092; -.
DR Reactome; R-MMU-176187; Activation of ATR in response to replication stress.
DR Reactome; R-MMU-68616; Assembly of the ORC complex at the origin of replication.
DR Reactome; R-MMU-68689; CDC6 association with the ORC:origin complex.
DR Reactome; R-MMU-68949; Orc1 removal from chromatin.
DR Reactome; R-MMU-68962; Activation of the pre-replicative complex.
DR BioGRID-ORCS; 18393; 4 hits in 74 CRISPR screens.
DR ChiTaRS; Orc2; mouse.
DR PRO; PR:Q60862; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; Q60862; protein.
DR Bgee; ENSMUSG00000026037; Expressed in spermatocyte and 251 other tissues.
DR ExpressionAtlas; Q60862; baseline and differential.
DR Genevisible; Q60862; MM.
DR GO; GO:0005813; C:centrosome; ISO:MGI.
DR GO; GO:0000785; C:chromatin; IDA:MGI.
DR GO; GO:0000781; C:chromosome, telomeric region; ISO:MGI.
DR GO; GO:0000792; C:heterochromatin; IDA:MGI.
DR GO; GO:0000939; C:inner kinetochore; IDA:MGI.
DR GO; GO:0005664; C:nuclear origin of replication recognition complex; IDA:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0000808; C:origin recognition complex; ISO:MGI.
DR GO; GO:0003688; F:DNA replication origin binding; IDA:MGI.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR InterPro; IPR007220; ORC2.
DR PANTHER; PTHR14052; PTHR14052; 1.
DR Pfam; PF04084; ORC2; 1.
PE 1: Evidence at protein level;
KW DNA replication; Nucleus; Phosphoprotein; Reference proteome.
FT CHAIN 1..576
FT /note="Origin recognition complex subunit 2"
FT /id="PRO_0000127076"
FT REPEAT 1..100
FT /note="Involved in LRWD1-binding"
FT /evidence="ECO:0000250"
FT REGION 112..233
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 112..127
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 129..167
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 183..197
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 116
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q13416"
FT MOD_RES 225
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q13416"
FT MOD_RES 247
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q13416"
SQ SEQUENCE 576 AA; 65893 MW; A18BA2D18E72553B CRC64;
MSTLQLKETK VPSVQFVGDD DVLSHILDRE GGTKLKKEKA QLLVNPQKVI KKADCELEKS
DLEVLEDQNY VKVLGRNIQE SLGNGSAKDG RNKVYSFQQR KHPEEMTKLA LELAKTSGKK
DPLDSNDPEI TKNIAQKSKG HSTSEKAPLV NNNKTEFLST QPHNLRKRII ASRSHYDSES
EYSASSSEDD EEATKDEEED TNVARLSQKS QGQNRLLPAP VSKETLPKKK KRDKASDLVE
EYFEAHSSSK VLTSDRTLQR LRRARVDQKT LHNLLRKFVP SFSAEIERLN QQHEKLFHKW
MLQLHLGFNI VLYGLGSKRD LLEKFRTTML QDSIHVVING YFPGVSVKSI LNSITEDVLS
HVGTFQSVLD QRDWIINRFK EDSSLELFLL IHNLDSQMLR GDNSQQILGQ LSSLHNVYLI
ASIDHLNAPL MWDHAKQSLY NWLWYETTTY SPYTEETSYE NSLLVKQSGS LPLSSLIHVL
RSLTPNARGI FRLLMKFQLD NQDSPSYIGL SFQDFYQQCR EAFLVNSDLT LRAQLTEFRD
HKLIRTKKGT DGVEYLLIPV DSGILADFLE KEEEEA
