ORC2_RAT
ID ORC2_RAT Reviewed; 576 AA.
AC Q75PQ8;
DT 29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=Origin recognition complex subunit 2;
GN Name=Orc2; Synonyms=Orc2l;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Saitoh Y., Tsutsumi K.;
RT "Rat origin recognition complex, subunit 2.";
RL Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Component of the origin recognition complex (ORC) that binds
CC origins of replication. DNA-binding is ATP-dependent. The specific DNA
CC sequences that define origins of replication have not been identified
CC yet. ORC is required to assemble the pre-replication complex necessary
CC to initiate DNA replication (By similarity). Binds histone H3 and H4
CC trimethylation marks H3K9me3, H3K20me3 and H4K27me3. Stabilizes LRWD1,
CC by protecting it from ubiquitin-mediated proteasomal degradation. Also
CC stabilizes ORC3 (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Component of ORC, a complex composed of at least 6 subunits:
CC ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6. ORC is regulated in a cell-cycle
CC dependent manner. It is sequentially assembled at the exit from
CC anaphase of mitosis and disassembled as cells enter S phase (By
CC similarity). Interacts with DBF4 (By similarity). Interacts with MCM10.
CC Interacts with LRWD1 throughout the cell cycle; this interaction, wich
CC occurs only with non-ubiquitinated form of LRWD1, prevents LRWD1
CC ubiquitination and hence stabilizes the protein. Interacts with POLQ
CC (By similarity). {ECO:0000250|UniProtKB:Q13416,
CC ECO:0000250|UniProtKB:Q60862}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the ORC2 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB164704; BAD12235.1; -; mRNA.
DR RefSeq; NP_001012003.1; NM_001012003.1.
DR RefSeq; XP_006245018.1; XM_006244956.3.
DR RefSeq; XP_017451848.1; XM_017596359.1.
DR AlphaFoldDB; Q75PQ8; -.
DR SMR; Q75PQ8; -.
DR STRING; 10116.ENSRNOP00000042867; -.
DR PhosphoSitePlus; Q75PQ8; -.
DR PaxDb; Q75PQ8; -.
DR Ensembl; ENSRNOT00000051843; ENSRNOP00000042867; ENSRNOG00000012558.
DR GeneID; 301430; -.
DR KEGG; rno:301430; -.
DR UCSC; RGD:1311844; rat.
DR CTD; 4999; -.
DR RGD; 1311844; Orc2.
DR eggNOG; KOG2928; Eukaryota.
DR GeneTree; ENSGT00390000015228; -.
DR HOGENOM; CLU_018596_3_0_1; -.
DR InParanoid; Q75PQ8; -.
DR OMA; FPQWAFE; -.
DR OrthoDB; 1404162at2759; -.
DR PhylomeDB; Q75PQ8; -.
DR TreeFam; TF101092; -.
DR Reactome; R-RNO-176187; Activation of ATR in response to replication stress.
DR Reactome; R-RNO-68616; Assembly of the ORC complex at the origin of replication.
DR Reactome; R-RNO-68689; CDC6 association with the ORC:origin complex.
DR Reactome; R-RNO-68949; Orc1 removal from chromatin.
DR Reactome; R-RNO-68962; Activation of the pre-replicative complex.
DR PRO; PR:Q75PQ8; -.
DR Proteomes; UP000002494; Chromosome 9.
DR Bgee; ENSRNOG00000012558; Expressed in thymus and 20 other tissues.
DR Genevisible; Q75PQ8; RN.
DR GO; GO:0005813; C:centrosome; ISO:RGD.
DR GO; GO:0000785; C:chromatin; ISO:RGD.
DR GO; GO:0000781; C:chromosome, telomeric region; IEA:Ensembl.
DR GO; GO:0000792; C:heterochromatin; ISO:RGD.
DR GO; GO:0000939; C:inner kinetochore; ISO:RGD.
DR GO; GO:0005664; C:nuclear origin of replication recognition complex; ISO:RGD.
DR GO; GO:0005654; C:nucleoplasm; IEA:Ensembl.
DR GO; GO:0005634; C:nucleus; ISO:RGD.
DR GO; GO:0000808; C:origin recognition complex; ISO:RGD.
DR GO; GO:0003688; F:DNA replication origin binding; ISO:RGD.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR InterPro; IPR007220; ORC2.
DR PANTHER; PTHR14052; PTHR14052; 1.
DR Pfam; PF04084; ORC2; 1.
PE 2: Evidence at transcript level;
KW DNA replication; Nucleus; Phosphoprotein; Reference proteome.
FT CHAIN 1..576
FT /note="Origin recognition complex subunit 2"
FT /id="PRO_0000329973"
FT REPEAT 1..100
FT /note="Involved in LRWD1-binding"
FT /evidence="ECO:0000250"
FT REGION 119..218
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 132..148
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 182..196
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 116
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q13416"
FT MOD_RES 246
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q13416"
SQ SEQUENCE 576 AA; 65850 MW; B9EF9FBBD2AD2BA2 CRC64;
MSTLRLKEAK VPSVQFVGDD DVLSHILDRE GGTKLKKEKV QLLVNPQKVI KKAECELEKS
DLEVLEDQNY VEVLGRNIQE SLGNGSAVDG RNKVYSFQHR KHPEEMTKLA LELAKTSGKI
DPLASNDPKI TKNIARKSKG HSSEKAPLEN NNKNEFPSIQ PHNVRKRLIA SRSHCDSESE
YSASSSEDDE EVAKDDEEDT NVVRCSKKSQ GQNRLLPAPV SKEILPKKRK RDKAGDLVEE
YFEAHSSSKV LTSDRTLQKL RRARVDQKTL RNLLSKFVPS FSTEIGQLNQ QHERLFDKWI
LQLRLGFNIV LYGLGSKRDL LEKFRTTMLQ DSIHVVINGF FPGISVKSIL NSITEDVLSH
MGTFQSVLDQ RDWIMNRFKE DSSLELFLLI HNLDSQMLRG DNSQQILGQL SSLRNVYLIA
SIDHLNAPLM WDHAKQSLYN WLWYETTTYS PYTEETSYEN SLLVKQSGSL PLSSLIHVLR
SLTPNARGIF RLLMKYQLDN QDCPSYIGLS FQDFYQQCRE AFLVNSDLTL RAQLTEFRDH
KLIRTKKGTD GVEYLLIPVD SGTLADFLEK EEEEES
