ORC2_YEAST
ID ORC2_YEAST Reviewed; 620 AA.
AC P32833; D6VQ60;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 2.
DT 03-AUG-2022, entry version 183.
DE RecName: Full=Origin recognition complex subunit 2;
DE AltName: Full=Origin recognition complex 71 kDa subunit;
GN Name=ORC2; Synonyms=RRR1, SIR5; OrderedLocusNames=YBR060C;
GN ORFNames=YBR0523;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE.
RX PubMed=8266071; DOI=10.1126/science.8266071;
RA Foss M., McNally F.J., Laurenson P., Rine J.;
RT "Origin recognition complex (ORC) in transcriptional silencing and DNA
RT replication in S. cerevisiae.";
RL Science 262:1838-1844(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8232543; DOI=10.1038/366087a0;
RA Micklem G., Rowley A., Harwood J., Nasmyth K., Diffley J.F.X.;
RT "Yeast origin recognition complex is involved in DNA replication and
RT transcriptional silencing.";
RL Nature 366:87-89(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7813418; DOI=10.1002/j.1460-2075.1994.tb06923.x;
RA Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C.,
RA Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M.,
RA Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M.,
RA Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H.,
RA Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D.,
RA Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N.,
RA Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J.,
RA Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C.,
RA Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P.,
RA Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y.,
RA Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F.,
RA Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E.,
RA Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M.,
RA Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B.,
RA Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L.,
RA Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M.,
RA Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S.,
RA Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K.,
RA Mewes H.-W., Kleine K.;
RT "Complete DNA sequence of yeast chromosome II.";
RL EMBO J. 13:5795-5809(1994).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP PARTIAL PROTEIN SEQUENCE, AND CHARACTERIZATION.
RX PubMed=8266072; DOI=10.1126/science.8266072;
RA Bell S.P., Kobayashi R., Stillman B.;
RT "Yeast origin recognition complex functions in transcription silencing and
RT DNA replication.";
RL Science 262:1844-1849(1993).
RN [6]
RP INTERACTION MCM10.
RX PubMed=11168584; DOI=10.1046/j.1365-2443.2000.00387.x;
RA Kawasaki Y., Hiraga S., Sugino A.;
RT "Interactions between Mcm10p and other replication factors are required for
RT proper initiation and elongation of chromosomal DNA replication in
RT Saccharomyces cerevisiae.";
RL Genes Cells 5:975-989(2000).
RN [7]
RP FUNCTION, AND INTERACTION WITH TAH11.
RX PubMed=17825064; DOI=10.1111/j.1567-1364.2007.00299.x;
RA Asano T., Makise M., Takehara M., Mizushima T.;
RT "Interaction between ORC and Cdt1p of Saccharomyces cerevisiae.";
RL FEMS Yeast Res. 7:1256-1262(2007).
RN [8]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [9]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [10]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-60; THR-187 AND SER-188, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- FUNCTION: Component of the origin recognition complex (ORC) that binds
CC origins of replication. It has a role in both chromosomal replication
CC and mating type transcriptional silencing. Binds to the ARS consensus
CC sequence (ACS) of origins of replication.
CC {ECO:0000269|PubMed:17825064}.
CC -!- SUBUNIT: Component of the origin recognition complex (ORC) composed of
CC at least ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6. Interacts with MCM10
CC and TAH11. {ECO:0000269|PubMed:11168584, ECO:0000269|PubMed:17825064}.
CC -!- INTERACTION:
CC P32833; P54790: ORC3; NbExp=6; IntAct=EBI-12572, EBI-12576;
CC P32833; P50874: ORC5; NbExp=4; IntAct=EBI-12572, EBI-12584;
CC P32833; P38826: ORC6; NbExp=6; IntAct=EBI-12572, EBI-12588;
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- MISCELLANEOUS: Present with 1700 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the ORC2 family. {ECO:0000305}.
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DR EMBL; L23924; AAA16325.1; -; Unassigned_DNA.
DR EMBL; Z21817; CAA79883.1; -; Genomic_DNA.
DR EMBL; Z35929; CAA85003.1; -; Genomic_DNA.
DR EMBL; BK006936; DAA07180.1; -; Genomic_DNA.
DR PIR; S38994; S38994.
DR RefSeq; NP_009616.1; NM_001178408.1.
DR PDB; 5V8F; EM; 3.90 A; B=1-620.
DR PDB; 5ZR1; EM; 3.00 A; B=1-620.
DR PDB; 6RQC; EM; 4.40 A; B=1-620.
DR PDB; 6WGC; EM; 4.30 A; B=1-620.
DR PDB; 6WGG; EM; 8.10 A; B=1-620.
DR PDB; 6WGI; EM; 10.00 A; B=1-620.
DR PDB; 7MCA; EM; 3.60 A; B=1-620.
DR PDBsum; 5V8F; -.
DR PDBsum; 5ZR1; -.
DR PDBsum; 6RQC; -.
DR PDBsum; 6WGC; -.
DR PDBsum; 6WGG; -.
DR PDBsum; 6WGI; -.
DR PDBsum; 7MCA; -.
DR AlphaFoldDB; P32833; -.
DR SMR; P32833; -.
DR BioGRID; 32764; 691.
DR ComplexPortal; CPX-768; Nuclear origin recognition complex.
DR DIP; DIP-2285N; -.
DR IntAct; P32833; 17.
DR MINT; P32833; -.
DR STRING; 4932.YBR060C; -.
DR iPTMnet; P32833; -.
DR MaxQB; P32833; -.
DR PaxDb; P32833; -.
DR PRIDE; P32833; -.
DR TopDownProteomics; P32833; -.
DR EnsemblFungi; YBR060C_mRNA; YBR060C; YBR060C.
DR GeneID; 852352; -.
DR KEGG; sce:YBR060C; -.
DR SGD; S000000264; ORC2.
DR VEuPathDB; FungiDB:YBR060C; -.
DR eggNOG; KOG2928; Eukaryota.
DR GeneTree; ENSGT00390000015228; -.
DR HOGENOM; CLU_022671_0_0_1; -.
DR InParanoid; P32833; -.
DR OMA; FIEHKMA; -.
DR BioCyc; YEAST:G3O-29031-MON; -.
DR Reactome; R-SCE-68616; Assembly of the ORC complex at the origin of replication.
DR Reactome; R-SCE-68689; CDC6 association with the ORC:origin complex.
DR Reactome; R-SCE-68949; Orc1 removal from chromatin.
DR Reactome; R-SCE-68962; Activation of the pre-replicative complex.
DR PRO; PR:P32833; -.
DR Proteomes; UP000002311; Chromosome II.
DR RNAct; P32833; protein.
DR GO; GO:0000781; C:chromosome, telomeric region; IEA:GOC.
DR GO; GO:0031261; C:DNA replication preinitiation complex; IDA:SGD.
DR GO; GO:0005664; C:nuclear origin of replication recognition complex; IDA:SGD.
DR GO; GO:0005656; C:nuclear pre-replicative complex; IDA:SGD.
DR GO; GO:0005654; C:nucleoplasm; TAS:Reactome.
DR GO; GO:0005634; C:nucleus; IC:ComplexPortal.
DR GO; GO:0003682; F:chromatin binding; IDA:SGD.
DR GO; GO:0003688; F:DNA replication origin binding; IDA:SGD.
DR GO; GO:0006270; P:DNA replication initiation; IMP:SGD.
DR GO; GO:0051568; P:histone H3-K4 methylation; IMP:SGD.
DR GO; GO:0006267; P:pre-replicative complex assembly involved in nuclear cell cycle DNA replication; IDA:SGD.
DR GO; GO:0030466; P:silent mating-type cassette heterochromatin assembly; IDA:SGD.
DR GO; GO:0031509; P:subtelomeric heterochromatin assembly; IMP:SGD.
DR InterPro; IPR007220; ORC2.
DR PANTHER; PTHR14052; PTHR14052; 1.
DR Pfam; PF04084; ORC2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; DNA replication; Nucleus;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..620
FT /note="Origin recognition complex subunit 2"
FT /id="PRO_0000127082"
FT REGION 1..184
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 204..225
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 24..45
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 80..116
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 130..146
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 147..167
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 60
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT MOD_RES 187
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT MOD_RES 188
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT HELIX 243..249
FT /evidence="ECO:0007829|PDB:5ZR1"
FT TURN 255..257
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 271..284
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 287..296
FT /evidence="ECO:0007829|PDB:5ZR1"
FT TURN 297..300
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 301..308
FT /evidence="ECO:0007829|PDB:5ZR1"
FT TURN 309..311
FT /evidence="ECO:0007829|PDB:5ZR1"
FT STRAND 313..317
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 323..331
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 334..341
FT /evidence="ECO:0007829|PDB:5ZR1"
FT STRAND 360..364
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 372..383
FT /evidence="ECO:0007829|PDB:5ZR1"
FT TURN 390..392
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 399..411
FT /evidence="ECO:0007829|PDB:5ZR1"
FT STRAND 420..425
FT /evidence="ECO:0007829|PDB:5ZR1"
FT TURN 430..432
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 435..445
FT /evidence="ECO:0007829|PDB:5ZR1"
FT STRAND 450..455
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 461..464
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 467..473
FT /evidence="ECO:0007829|PDB:5ZR1"
FT STRAND 475..479
FT /evidence="ECO:0007829|PDB:5ZR1"
FT TURN 487..490
FT /evidence="ECO:0007829|PDB:5ZR1"
FT STRAND 491..493
FT /evidence="ECO:0007829|PDB:5ZR1"
FT TURN 495..499
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 507..514
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 519..539
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 550..552
FT /evidence="ECO:0007829|PDB:5ZR1"
FT STRAND 553..555
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 556..565
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 572..583
FT /evidence="ECO:0007829|PDB:5ZR1"
FT TURN 584..586
FT /evidence="ECO:0007829|PDB:5ZR1"
FT STRAND 587..592
FT /evidence="ECO:0007829|PDB:5ZR1"
FT STRAND 598..601
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 606..615
FT /evidence="ECO:0007829|PDB:5ZR1"
FT TURN 616..619
FT /evidence="ECO:0007829|PDB:5ZR1"
SQ SEQUENCE 620 AA; 71238 MW; 486E3E5CE353807F CRC64;
MLNGEDFVEH NDILSSPAKS RNVTPKRVDP HGERQLRRIH SSKKNLLERI SLVGNERKNT
SPDPALKPKT PSKAPRKRGR PRKIQEELTD RIKKDEKDTI SSKKKRKLDK DTSGNVNEES
KTSNNKQVME KTGIKEKRER EKIQVATTTY EDNVTPQTDD NFVSNSPEPP EPATPSKKSL
TTNHDFTSPL KQIIMNNLKE YKDSTSPGKL TLSRNFTPTP VPKNKKLYQT SETKSASSFL
DTFEGYFDQR KIVRTNAKSR HTMSMAPDVT REEFSLVSNF FNENFQKRPR QKLFEIQKKM
FPQYWFELTQ GFSLLFYGVG SKRNFLEEFA IDYLSPKIAY SQLAYENELQ QNKPVNSIPC
LILNGYNPSC NYRDVFKEIT DLLVPAELTR SETKYWGNHV ILQIQKMIDF YKNQPLDIKL
ILVVHNLDGP SIRKNTFQTM LSFLSVIRQI AIVASTDHIY APLLWDNMKA QNYNFVFHDI
SNFEPSTVES TFQDVMKMGK SDTSSGAEGA KYVLQSLTVN SKKMYKLLIE TQMQNMGNLS
ANTGPKRGTQ RTGVELKLFN HLCAADFIAS NEIALRSMLR EFIEHKMANI TKNNSGMEII
WVPYTYAELE KLLKTVLNTL
