ORC3_ARATH
ID ORC3_ARATH Reviewed; 734 AA.
AC Q6E7H0; Q9LFE8;
DT 07-JAN-2015, integrated into UniProtKB/Swiss-Prot.
DT 16-AUG-2004, sequence version 1.
DT 25-MAY-2022, entry version 110.
DE RecName: Full=Origin of replication complex subunit 3 {ECO:0000303|PubMed:16179646};
DE Short=AtORC3 {ECO:0000303|PubMed:16179646};
GN Name=ORC3 {ECO:0000303|PubMed:16179646};
GN OrderedLocusNames=At5g16690 {ECO:0000312|Araport:AT5G16690};
GN ORFNames=F5E19.30 {ECO:0000312|EMBL:CAC01833.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|EMBL:AAT37463.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], SUBUNIT, INTERACTION WITH ORC3; ORC4; ORC5 AND
RP ORC6, TISSUE SPECIFICITY, INDUCTION BY SUCROSE, AND GENE FAMILY.
RX PubMed=15358564; DOI=10.1016/j.febslet.2004.07.088;
RA Masuda H.P., Ramos G.B.A., de Almeida-Engler J., Cabral L.M.,
RA Coqueiro V.M., Macrini C.M.T., Ferreira P.C.G., Hemerly A.S.;
RT "Genome based identification and analysis of the pre-replicative complex of
RT Arabidopsis thaliana.";
RL FEBS Lett. 574:192-202(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP SUBUNIT, INTERACTION WITH ORC1A; ORC2; ORC4; ORC5 AND ORC6, TISSUE
RP SPECIFICITY, INDUCTION BY E2F AND SUCROSE, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16179646; DOI=10.1093/nar/gki854;
RA Diaz-Trivino S., Castellano M.M., Sanchez M.P., Ramirez-Parra E.,
RA Desvoyes B., Gutierrez C.;
RT "The genes encoding Arabidopsis ORC subunits are E2F targets and the two
RT ORC1 genes are differently expressed in proliferating and endoreplicating
RT cells.";
RL Nucleic Acids Res. 33:5404-5414(2005).
RN [5]
RP INDUCTION BY E2F.
RX PubMed=16126853; DOI=10.1104/pp.105.066290;
RA Vandepoele K., Vlieghe K., Florquin K., Hennig L., Beemster G.T.S.,
RA Gruissem W., Van de Peer Y., Inze D., De Veylder L.;
RT "Genome-wide identification of potential plant E2F target genes.";
RL Plant Physiol. 139:316-328(2005).
RN [6]
RP REVIEW ON THE CORE DNA REPLICATION MACHINERY.
RX PubMed=17556508; DOI=10.1104/pp.107.101105;
RA Shultz R.W., Tatineni V.M., Hanley-Bowdoin L., Thompson W.F.;
RT "Genome-wide analysis of the core DNA replication machinery in the higher
RT plants Arabidopsis and rice.";
RL Plant Physiol. 144:1697-1714(2007).
CC -!- FUNCTION: Component of the origin recognition complex (ORC) that binds
CC origins of replication. DNA-binding is ATP-dependent. The specific DNA
CC sequences that define origins of replication have not been identified
CC yet. {ECO:0000250|UniProtKB:Q9UBD5}.
CC -!- SUBUNIT: Component of the origin recognition complex (ORC) composed of
CC at least ORC1 (ORC1A or ORC1B), ORC2, ORC3, ORC4, ORC5 and ORC6. ORC is
CC regulated in a cell-cycle and development dependent manner. It is
CC sequentially assembled at the exit from anaphase of mitosis and
CC disassembled as cells enter S phase. Interacts directly with ORC1A,
CC ORC2, ORC4, ORC5 and ORC6. {ECO:0000269|PubMed:15358564,
CC ECO:0000269|PubMed:16179646}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9UBD5}.
CC -!- TISSUE SPECIFICITY: Follow a cell-cycle regulation with a peak at the
CC G1/S-phase (PubMed:16179646). Mostly expressed in siliques and flowers,
CC and, to a lower exent, in flower buds, leaves, roots and stems
CC (PubMed:16179646, PubMed:15358564). {ECO:0000269|PubMed:15358564,
CC ECO:0000269|PubMed:16179646}.
CC -!- INDUCTION: Regulated by E2F (PubMed:16179646, PubMed:16126853).
CC Accumulates rapidly after cell cycle reactivation by sucrose addition
CC following cell cycle arrest mediated by sucrose deprivation
CC (PubMed:16179646, PubMed:15358564). {ECO:0000269|PubMed:15358564,
CC ECO:0000269|PubMed:16126853, ECO:0000269|PubMed:16179646}.
CC -!- SIMILARITY: Belongs to the ORC3 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAC01833.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AY524002; AAT37463.1; -; mRNA.
DR EMBL; AL391147; CAC01833.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED92326.1; -; Genomic_DNA.
DR PIR; T51501; T51501.
DR RefSeq; NP_197171.2; NM_121674.4.
DR AlphaFoldDB; Q6E7H0; -.
DR SMR; Q6E7H0; -.
DR IntAct; Q6E7H0; 7.
DR MINT; Q6E7H0; -.
DR STRING; 3702.AT5G16690.1; -.
DR iPTMnet; Q6E7H0; -.
DR PaxDb; Q6E7H0; -.
DR PRIDE; Q6E7H0; -.
DR ProteomicsDB; 248641; -.
DR EnsemblPlants; AT5G16690.1; AT5G16690.1; AT5G16690.
DR GeneID; 831530; -.
DR Gramene; AT5G16690.1; AT5G16690.1; AT5G16690.
DR KEGG; ath:AT5G16690; -.
DR Araport; AT5G16690; -.
DR TAIR; locus:2148995; AT5G16690.
DR eggNOG; KOG2538; Eukaryota.
DR HOGENOM; CLU_010669_0_0_1; -.
DR InParanoid; Q6E7H0; -.
DR OMA; HLECGRM; -.
DR OrthoDB; 1196817at2759; -.
DR PhylomeDB; Q6E7H0; -.
DR PRO; PR:Q6E7H0; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q6E7H0; baseline and differential.
DR Genevisible; Q6E7H0; AT.
DR GO; GO:0031261; C:DNA replication preinitiation complex; IBA:GO_Central.
DR GO; GO:0005664; C:nuclear origin of replication recognition complex; IBA:GO_Central.
DR GO; GO:0005656; C:nuclear pre-replicative complex; IBA:GO_Central.
DR GO; GO:0000808; C:origin recognition complex; ISS:TAIR.
DR GO; GO:0003688; F:DNA replication origin binding; IBA:GO_Central.
DR GO; GO:0006260; P:DNA replication; ISS:TAIR.
DR GO; GO:0006270; P:DNA replication initiation; IBA:GO_Central.
DR GO; GO:0048527; P:lateral root development; IEA:EnsemblPlants.
DR GO; GO:0009744; P:response to sucrose; IEA:EnsemblPlants.
DR InterPro; IPR020795; ORC3.
DR InterPro; IPR045667; ORC3_N.
DR InterPro; IPR040855; ORC_WH_C.
DR PANTHER; PTHR12748; PTHR12748; 1.
DR Pfam; PF07034; ORC3_N; 1.
DR Pfam; PF18137; ORC_WH_C; 1.
PE 1: Evidence at protein level;
KW DNA replication; DNA-binding; Nucleus; Reference proteome.
FT CHAIN 1..734
FT /note="Origin of replication complex subunit 3"
FT /id="PRO_0000431431"
FT REGION 1..25
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 532..554
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 8..25
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 734 AA; 82755 MW; FDE04D7FA55F769D CRC64;
MAPSGTVADP PQCSTTDSFN SSDTAENDIR PFFVLHKASS GNHNGKLTGI VKSKRRIESP
SPKIAKRSEV ESVEEEDGQF FSTLRFKVFE TVWSKIEKTI EDVLRNSNSK VFSGIHDWIR
ESFESIISSG ALKLSEAVRS YPVLTQASSK QLLTAMVLTR NLEMVDDLLT FEELELHLKS
QGCHVAKLSS MDFSAKSGVG GCLRGLLRQF VMPTVDVADV TILASWYREN KNHENPVVII
VDDTERCCGP VLSDLILILS EWAIKVPIFL IMGVSTAHDA PRKILSVNAL QRLCATRFTL
SSPAERMDAV LKAVFLKPCS GFTVSHKVAL FMRSYFLCQD GTLTSFVRTL KIACLQHFSL
EPLSIMLEHF CHDGVNQLSG EGTELLTEAT MKHAFDLPSV TRNKITRSTF EMLPHFLLDL
QRMPNPWSIV VLCLYEAGKF DKLRLLDIFC EILDPEARYL KYFSPSEIVN SQSHNSGRNN
VIRRVLRKLR DLSPSQLSSM LKSWENLTAE FTEINDKVIE LHPFMRAVEA AGQRQGLPNS
PKKHASRSNS KLEKELKAMT DKVATVIEFM LREYMKPVES VPFHEILCFK NVDKLQSALL
GDPRGRIQLD LLESHDILHC VCCSQRGTTL LPSMHDTSIL YKLAQEHADV INLHDWYQSF
KTILIPRSSK AKQKSKSSSK SKKRKEICEE PEAPAEALIQ ARFCRAVMEL QITGLIRMPS
KRRPDFVQRV AFGS
