ORC3_MOUSE
ID ORC3_MOUSE Reviewed; 715 AA.
AC Q9JK30; A2AMF4; A2AMF5; Q6P8H2; Q8BR83; Q8C395;
DT 24-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Origin recognition complex subunit 3;
DE AltName: Full=Origin recognition complex subunit Latheo;
GN Name=Orc3; Synonyms=Orc3l;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX PubMed=10702681; DOI=10.1159/000015435;
RA Springer J., Nanda I., Hoehn K., Schmid M., Grummt F.;
RT "Identification and chromosomal localization of murine ORC3, a new member
RT of the mouse origin recognition complex.";
RL Cytogenet. Cell Genet. 87:245-251(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RX PubMed=15634681; DOI=10.1074/jbc.m413280200;
RA Miyake Y., Mizuno T., Yanagi K., Hanaoka F.;
RT "Novel splicing variant of mouse Orc1 is deficient in nuclear translocation
RT and resistant for proteasome-mediated degradation.";
RL J. Biol. Chem. 280:12643-12652(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Corpora quadrigemina, and Head;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Pituitary;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-522 (ISOFORM 2), AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Embryonic fibroblast;
RX PubMed=17525332; DOI=10.1126/science.1140321;
RA Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E.,
RA Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y.,
RA Gygi S.P., Elledge S.J.;
RT "ATM and ATR substrate analysis reveals extensive protein networks
RT responsive to DNA damage.";
RL Science 316:1160-1166(2007).
CC -!- FUNCTION: Component of the origin recognition complex (ORC) that binds
CC origins of replication. DNA-binding is ATP-dependent. The specific DNA
CC sequences that define origins of replication have not been identified
CC yet. ORC is required to assemble the pre-replication complex necessary
CC to initiate DNA replication. Binds histone H3 and H4 trimethylation
CC marks H3K9me3, H3K27me3 and H4K20me3. {ECO:0000250|UniProtKB:Q9UBD5}.
CC -!- SUBUNIT: Component of ORC, a complex composed of at least 6 subunits:
CC ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6. ORC is regulated in a cell-cycle
CC dependent manner. It is sequentially assembled at the exit from
CC anaphase of mitosis and disassembled as cells enter S phase.
CC {ECO:0000250|UniProtKB:Q9UBD5}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9UBD5}.
CC Chromosome {ECO:0000250|UniProtKB:Q9UBD5}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9JK30-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9JK30-2; Sequence=VSP_026407;
CC -!- PTM: Multi-mono-ubiquitinated by OBI1; ubiquitination is important for
CC efficient DNA replication origin site activation. Ubiquitination levels
CC are low in mitotic and early G1-phAse cells and are induced in late
CC G1-/early S-phase, peaking in S-phase and decrease toward the end of
CC the cell cycle. {ECO:0000250|UniProtKB:Q9UBD5}.
CC -!- SIMILARITY: Belongs to the ORC3 family. {ECO:0000305}.
CC -!- CAUTION: It is uncertain whether Met-1 or Met-8 is the initiator.
CC {ECO:0000305}.
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DR EMBL; AJ132830; CAB76399.1; -; mRNA.
DR EMBL; AB190257; BAD91665.2; -; mRNA.
DR EMBL; AK045388; BAC32339.1; -; mRNA.
DR EMBL; AK086579; BAC39695.1; -; mRNA.
DR EMBL; AL807397; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL928738; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC061252; AAH61252.1; -; mRNA.
DR CCDS; CCDS18028.1; -. [Q9JK30-1]
DR CCDS; CCDS51137.1; -. [Q9JK30-2]
DR RefSeq; NP_001153035.1; NM_001159563.1. [Q9JK30-2]
DR RefSeq; NP_056639.3; NM_015824.4. [Q9JK30-1]
DR AlphaFoldDB; Q9JK30; -.
DR SMR; Q9JK30; -.
DR BioGRID; 206124; 4.
DR ComplexPortal; CPX-1915; Nuclear origin of replication recognition complex.
DR CORUM; Q9JK30; -.
DR STRING; 10090.ENSMUSP00000048319; -.
DR iPTMnet; Q9JK30; -.
DR PhosphoSitePlus; Q9JK30; -.
DR EPD; Q9JK30; -.
DR MaxQB; Q9JK30; -.
DR PaxDb; Q9JK30; -.
DR PeptideAtlas; Q9JK30; -.
DR PRIDE; Q9JK30; -.
DR ProteomicsDB; 294107; -. [Q9JK30-1]
DR ProteomicsDB; 294108; -. [Q9JK30-2]
DR Antibodypedia; 18665; 244 antibodies from 29 providers.
DR DNASU; 50793; -.
DR Ensembl; ENSMUST00000048706; ENSMUSP00000048319; ENSMUSG00000040044. [Q9JK30-1]
DR Ensembl; ENSMUST00000108142; ENSMUSP00000103777; ENSMUSG00000040044. [Q9JK30-2]
DR GeneID; 50793; -.
DR KEGG; mmu:50793; -.
DR UCSC; uc008sgb.2; mouse. [Q9JK30-1]
DR UCSC; uc008sgd.1; mouse. [Q9JK30-2]
DR CTD; 23595; -.
DR MGI; MGI:1354944; Orc3.
DR VEuPathDB; HostDB:ENSMUSG00000040044; -.
DR eggNOG; KOG2538; Eukaryota.
DR GeneTree; ENSGT00390000011376; -.
DR HOGENOM; CLU_015257_2_0_1; -.
DR InParanoid; Q9JK30; -.
DR OMA; HLECGRM; -.
DR OrthoDB; 1196817at2759; -.
DR PhylomeDB; Q9JK30; -.
DR TreeFam; TF101093; -.
DR Reactome; R-MMU-176187; Activation of ATR in response to replication stress.
DR Reactome; R-MMU-68616; Assembly of the ORC complex at the origin of replication.
DR Reactome; R-MMU-68689; CDC6 association with the ORC:origin complex.
DR Reactome; R-MMU-68949; Orc1 removal from chromatin.
DR Reactome; R-MMU-68962; Activation of the pre-replicative complex.
DR BioGRID-ORCS; 50793; 19 hits in 73 CRISPR screens.
DR ChiTaRS; Orc3; mouse.
DR PRO; PR:Q9JK30; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; Q9JK30; protein.
DR Bgee; ENSMUSG00000040044; Expressed in undifferentiated genital tubercle and 254 other tissues.
DR ExpressionAtlas; Q9JK30; baseline and differential.
DR Genevisible; Q9JK30; MM.
DR GO; GO:0000785; C:chromatin; ISS:UniProtKB.
DR GO; GO:0031261; C:DNA replication preinitiation complex; IBA:GO_Central.
DR GO; GO:0016604; C:nuclear body; ISO:MGI.
DR GO; GO:0005664; C:nuclear origin of replication recognition complex; ISS:UniProtKB.
DR GO; GO:0005656; C:nuclear pre-replicative complex; IBA:GO_Central.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0000808; C:origin recognition complex; ISO:MGI.
DR GO; GO:0003688; F:DNA replication origin binding; IBA:GO_Central.
DR GO; GO:0006260; P:DNA replication; TAS:MGI.
DR GO; GO:0006270; P:DNA replication initiation; IBA:GO_Central.
DR GO; GO:0014009; P:glial cell proliferation; IMP:MGI.
DR GO; GO:0061351; P:neural precursor cell proliferation; IMP:MGI.
DR GO; GO:0006275; P:regulation of DNA replication; ISS:UniProtKB.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR020795; ORC3.
DR InterPro; IPR045663; ORC3_ins.
DR InterPro; IPR045667; ORC3_N.
DR InterPro; IPR040855; ORC_WH_C.
DR PANTHER; PTHR12748; PTHR12748; 1.
DR Pfam; PF19675; ORC3_ins; 1.
DR Pfam; PF07034; ORC3_N; 1.
DR Pfam; PF18137; ORC_WH_C; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Chromosome; DNA replication; DNA-binding; Nucleus;
KW Phosphoprotein; Reference proteome; Ubl conjugation.
FT CHAIN 1..715
FT /note="Origin recognition complex subunit 3"
FT /id="PRO_0000127084"
FT MOD_RES 523
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9UBD5"
FT VAR_SEQ 512
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:15634681"
FT /id="VSP_026407"
FT CONFLICT 114
FT /note="L -> F (in Ref. 3; BAC39695)"
FT /evidence="ECO:0000305"
FT CONFLICT 212
FT /note="A -> P (in Ref. 2; BAD91665 and 5; AAH61252)"
FT /evidence="ECO:0000305"
FT CONFLICT 360
FT /note="R -> G (in Ref. 3; BAC32339)"
FT /evidence="ECO:0000305"
FT CONFLICT 645
FT /note="L -> R (in Ref. 2; BAD91665 and 5; AAH61252)"
FT /evidence="ECO:0000305"
FT MOD_RES Q9JK30-2:522
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17525332"
SQ SEQUENCE 715 AA; 82342 MW; 1E915A719A2E33EB CRC64;
MHTGPRTMAT SSVSKGCFVF KPDFKKRKVF VPIEDYFNNE ELDSEDSKLR FETYSLLWQR
MKSETEQLQE ELNENLFDNL VDFLQKSHPE FQKNSRDWGS QMKFREIPTA ALILGVNVTD
HDVILRSLTE TLQNNVTPYV VSLQAKDCPD VKHFLQKFTS QLMDCCVDRH SKEVTSGKAL
KKTNYSMDSL CSWYSAVTQK ADHKVTIKKR TASGHWRSPP VVLILKSMES FTSKVLQDFI
TISSQHLHEF PLILIFGIAT SPVIIHRLLP HSVSSLLCVE LFQSLSCEQH LTVVLDKLLL
TPQFPFKLSK KALQVLTNIF LYHDFSIQSF IKGIKLSLLE HFYSQPLSVL CCDLSEAKKR
VNVFSVSQCE NIRRLPSFRR YVENQPLGKQ VALLTNETFL KEKTQSLLED LHVYHINYFL
VLRCLHNFTS SLPKYPLGRQ IRELYCTCLE KKIWDSEEYK SALQLLRMLA KDELVSILQR
CIEVLDSSTE KQLGNTTQKI KDFLTQFQNL DADSKEEEDA CGSQPKGLQK TDLYHLQKSL
LEMKELRRTK KPTKFEMLRE NVMNFIDNLV RDYLLPPESQ PLHEVVYFSA ANTLREHLNA
APRIALHTAL NNPYYYLKNE ELEGCIPNTA PDICIAYKLH LECSLLINLV DWAEAFATVV
TAAEKMDANS TVSEEMSEVI HARFIRAVSE LELLGFIKPT KQKTDHVARL TWGGC
