ORC3_SCHPO
ID ORC3_SCHPO Reviewed; 690 AA.
AC Q10067; Q9P7G2; Q9UTT0;
DT 01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT 14-AUG-2001, sequence version 2.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Origin recognition complex subunit 3;
GN Name=orc3; Synonyms=orp3; ORFNames=SPAC3H1.01c, SPAP14E8.06c;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 58-77; 80-89 AND 376-384,
RP AND SUBUNIT.
RX PubMed=10535928; DOI=10.1073/pnas.96.22.12367;
RA Moon K.-Y., Kong D., Lee J.-K., Raychaudhuri S., Hurwitz J.;
RT "Identification and reconstitution of the origin recognition complex from
RT Schizosaccharomyces pombe.";
RL Proc. Natl. Acad. Sci. U.S.A. 96:12367-12372(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [3]
RP CHARACTERIZATION OF ORC, AND INTERACTION WITH CDC18.
RX PubMed=11850415; DOI=10.1074/jbc.m107710200;
RA Chuang R.-Y., Chretien L., Dai J., Kelly T.J.;
RT "Purification and characterization of the Schizosaccharomyces pombe origin
RT recognition complex: interaction with origin DNA and Cdc18 protein.";
RL J. Biol. Chem. 277:16920-16927(2002).
CC -!- FUNCTION: Component of the origin recognition complex (ORC) that binds
CC origins of replication. It has a role in both chromosomal replication
CC and mating type transcriptional silencing. ORC binds to multiple sites
CC within the ars1 origin of DNA replication in an ATP-independent manner.
CC -!- SUBUNIT: ORC is composed of six subunits. ORC interacts with cdc18,
CC recruiting it to the ars1 origin of replication.
CC {ECO:0000269|PubMed:10535928, ECO:0000269|PubMed:11850415}.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- SIMILARITY: Belongs to the ORC3 family. {ECO:0000305}.
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DR EMBL; AF188642; AAF05949.1; -; mRNA.
DR EMBL; CU329670; CAB77007.1; -; Genomic_DNA.
DR PIR; T38733; T38733.
DR RefSeq; XP_001713061.1; XM_001713009.2.
DR AlphaFoldDB; Q10067; -.
DR SMR; Q10067; -.
DR BioGRID; 279676; 6.
DR STRING; 4896.SPAC3H1.01c.1; -.
DR iPTMnet; Q10067; -.
DR MaxQB; Q10067; -.
DR PaxDb; Q10067; -.
DR PRIDE; Q10067; -.
DR EnsemblFungi; SPAC3H1.01c.1; SPAC3H1.01c.1:pep; SPAC3H1.01c.
DR PomBase; SPAC3H1.01c; orc3.
DR VEuPathDB; FungiDB:SPAC3H1.01c; -.
DR eggNOG; KOG2538; Eukaryota.
DR HOGENOM; CLU_015257_1_0_1; -.
DR InParanoid; Q10067; -.
DR OMA; FYERSWS; -.
DR PhylomeDB; Q10067; -.
DR Reactome; R-SPO-176187; Activation of ATR in response to replication stress.
DR Reactome; R-SPO-68616; Assembly of the ORC complex at the origin of replication.
DR Reactome; R-SPO-68689; CDC6 association with the ORC:origin complex.
DR Reactome; R-SPO-68949; Orc1 removal from chromatin.
DR Reactome; R-SPO-68962; Activation of the pre-replicative complex.
DR PRO; PR:Q10067; -.
DR Proteomes; UP000002485; Chromosome I.
DR GO; GO:0000785; C:chromatin; IDA:PomBase.
DR GO; GO:0031261; C:DNA replication preinitiation complex; IBA:GO_Central.
DR GO; GO:0005664; C:nuclear origin of replication recognition complex; IDA:UniProtKB.
DR GO; GO:0005656; C:nuclear pre-replicative complex; IBA:GO_Central.
DR GO; GO:0043596; C:nuclear replication fork; IC:PomBase.
DR GO; GO:0003688; F:DNA replication origin binding; IDA:UniProtKB.
DR GO; GO:0006270; P:DNA replication initiation; IBA:GO_Central.
DR GO; GO:1902975; P:mitotic DNA replication initiation; IC:PomBase.
DR InterPro; IPR020795; ORC3.
DR InterPro; IPR045667; ORC3_N.
DR InterPro; IPR040855; ORC_WH_C.
DR PANTHER; PTHR12748; PTHR12748; 1.
DR Pfam; PF07034; ORC3_N; 1.
DR Pfam; PF18137; ORC_WH_C; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; DNA replication; DNA-binding; Nucleus;
KW Reference proteome.
FT CHAIN 1..690
FT /note="Origin recognition complex subunit 3"
FT /id="PRO_0000127085"
SQ SEQUENCE 690 AA; 80193 MW; 60801BF9642CE4F3 CRC64;
MSAILQYDSV SKGTYSVKPK PTESLGNEFS EGLFVPLIGG KEPIEYVKLR ENLCTKVLNE
LEEITTHTTY DSNARVLRQL CEHASEPVDE TGLLRTAIVC DKSSNSMHLK FYEQLKDDME
KIGWKNFVVL NLKAHRDLKS CFRVIKGGSF GLGVLSYDIE KLDEKTVLVL EDVEDCDRRL
LSSLVEALSS LVRHSKLQGC LYTIFNLKIP LEMFDTSLDS KFLSNVKTKV FNMKASTEIL
ESLFTSIEES LSLKFGWRTR RFFRSMFYER SWSVERVIEC IRYSILTHFY GNALSIIEHL
IYQKDFHLIS PLHLTTLRTV PSFQRHIEQR LEIGDLESIN YVEKMLNDDT YFQEMVIQMC
ENLLYRERLL RTQAKIWHEL EMAVKTTTRI SFADYLETFL QGDWLSSPQY KSICQAILRM
NSTKALACLD YLNENIFTGN QTMKCLEIHQ ELSELIRNSS TNYLEPVEVR MQNYSGTKHT
RKVVESGFDK SIIDFSKILK KIVGLLDEEV QYGCLGTESI DMYPFYEVLF YDYCRPLNQA
FASGHQRSVI HSSCMDPEYY VGDEKKIDSE FSNAEKDGKD LISWLPDLSI LYKLYSESGA
LLNLYDWYIA FSEHLQAGKE NLKEDDNHPR ESPQGNLQRE LNELDEDKRK LEEAKLQSRF
LFGLEELRFL GLIKPTARKT DHVMKTIYHQ
