ORC3_YEAST
ID ORC3_YEAST Reviewed; 616 AA.
AC P54790; D6VXZ9;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 162.
DE RecName: Full=Origin recognition complex subunit 3;
DE AltName: Full=Origin recognition complex 62 kDa subunit;
GN Name=ORC3; Synonyms=OAF1, OIF1; OrderedLocusNames=YLL004W; ORFNames=L1365;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RX PubMed=7585959; DOI=10.1016/0092-8674(95)90096-9;
RA Bell S.P., Mitchell J., Leber J., Kobayashi R., Stillman B.;
RT "The multidomain structure of Orc1p reveals similarity to regulators of DNA
RT replication and transcriptional silencing.";
RL Cell 83:563-568(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8657159; DOI=10.1128/mcb.16.4.1832;
RA Hardy C.F.J.;
RT "Characterization of an essential Orc2p-associated factor that plays a role
RT in DNA replication.";
RL Mol. Cell. Biol. 16:1832-1841(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 90840 / EAY235 / FY23;
RX PubMed=8810043;
RX DOI=10.1002/(sici)1097-0061(19960615)12:7<693::aid-yea956>3.0.co;2-g;
RA Miosga T., Zimmermann F.K.;
RT "Sequence analysis of the CEN12 region of Saccharomyces cerevisiae on a
RT 43.7 kb fragment of chromosome XII including an open reading frame
RT homologous to the human cystic fibrosis transmembrane conductance regulator
RT protein CFTR.";
RL Yeast 12:693-708(1996).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169871;
RA Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W.,
RA Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A.,
RA Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K.,
RA Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., Koetter P., Louis E.J.,
RA Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S.,
RA Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D.,
RA Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M.,
RA Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P.,
RA Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M.,
RA Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K.,
RA Zollner A., Hani J., Hoheisel J.D.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL Nature 387:87-90(1997).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [6]
RP FUNCTION, AND INTERACTION WITH ORC6.
RX PubMed=18006685; DOI=10.1101/gad.1596807;
RA Chen S., de Vries M.A., Bell S.P.;
RT "Orc6 is required for dynamic recruitment of Cdt1 during repeated Mcm2-7
RT loading.";
RL Genes Dev. 21:2897-2907(2007).
RN [7]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, CLEAVAGE OF INITIATOR
RP METHIONINE [LARGE SCALE ANALYSIS], AND IDENTIFICATION BY MASS SPECTROMETRY
RP [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- FUNCTION: Component of the origin recognition complex (ORC) that binds
CC origins of replication. It has a role in both chromosomal replication
CC and mating type transcriptional silencing. Binds to the ARS consensus
CC sequence (ACS) of origins of replication.
CC {ECO:0000269|PubMed:18006685}.
CC -!- SUBUNIT: Component of the origin recognition complex (ORC) composed of
CC at least ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6. Interacts with ORC6.
CC {ECO:0000269|PubMed:18006685}.
CC -!- INTERACTION:
CC P54790; P32833: ORC2; NbExp=6; IntAct=EBI-12576, EBI-12572;
CC P54790; P54791: ORC4; NbExp=3; IntAct=EBI-12576, EBI-12580;
CC P54790; P38826: ORC6; NbExp=4; IntAct=EBI-12576, EBI-12588;
CC P54790; Q12495: RLF2; NbExp=3; IntAct=EBI-12576, EBI-3913;
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- SIMILARITY: Belongs to the ORC3 family. {ECO:0000305}.
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DR EMBL; U34861; AAB38249.1; -; Genomic_DNA.
DR EMBL; U48888; AAC49270.1; -; Genomic_DNA.
DR EMBL; X91488; CAA62765.1; -; Genomic_DNA.
DR EMBL; Z73109; CAA97447.1; -; Genomic_DNA.
DR EMBL; BK006945; DAA09315.1; -; Genomic_DNA.
DR PIR; S64746; S64746.
DR RefSeq; NP_013097.1; NM_001181824.1.
DR PDB; 5V8F; EM; 3.90 A; C=1-616.
DR PDB; 5ZR1; EM; 3.00 A; C=1-616.
DR PDB; 6RQC; EM; 4.40 A; C=1-616.
DR PDB; 6WGC; EM; 4.30 A; C=1-616.
DR PDB; 6WGG; EM; 8.10 A; C=1-616.
DR PDB; 6WGI; EM; 10.00 A; C=1-616.
DR PDB; 7MCA; EM; 3.60 A; C=1-616.
DR PDBsum; 5V8F; -.
DR PDBsum; 5ZR1; -.
DR PDBsum; 6RQC; -.
DR PDBsum; 6WGC; -.
DR PDBsum; 6WGG; -.
DR PDBsum; 6WGI; -.
DR PDBsum; 7MCA; -.
DR AlphaFoldDB; P54790; -.
DR SMR; P54790; -.
DR BioGRID; 31247; 248.
DR ComplexPortal; CPX-768; Nuclear origin recognition complex.
DR DIP; DIP-2286N; -.
DR IntAct; P54790; 17.
DR MINT; P54790; -.
DR STRING; 4932.YLL004W; -.
DR iPTMnet; P54790; -.
DR MaxQB; P54790; -.
DR PaxDb; P54790; -.
DR PRIDE; P54790; -.
DR EnsemblFungi; YLL004W_mRNA; YLL004W; YLL004W.
DR GeneID; 850656; -.
DR KEGG; sce:YLL004W; -.
DR SGD; S000003927; ORC3.
DR VEuPathDB; FungiDB:YLL004W; -.
DR eggNOG; KOG2538; Eukaryota.
DR GeneTree; ENSGT00390000011376; -.
DR HOGENOM; CLU_403318_0_0_1; -.
DR InParanoid; P54790; -.
DR OMA; ESPNVRM; -.
DR BioCyc; YEAST:G3O-32109-MON; -.
DR Reactome; R-SCE-68616; Assembly of the ORC complex at the origin of replication.
DR Reactome; R-SCE-68689; CDC6 association with the ORC:origin complex.
DR Reactome; R-SCE-68949; Orc1 removal from chromatin.
DR Reactome; R-SCE-68962; Activation of the pre-replicative complex.
DR PRO; PR:P54790; -.
DR Proteomes; UP000002311; Chromosome XII.
DR RNAct; P54790; protein.
DR GO; GO:0031261; C:DNA replication preinitiation complex; IDA:SGD.
DR GO; GO:0005664; C:nuclear origin of replication recognition complex; IDA:SGD.
DR GO; GO:0005656; C:nuclear pre-replicative complex; IDA:SGD.
DR GO; GO:0005654; C:nucleoplasm; TAS:Reactome.
DR GO; GO:0005634; C:nucleus; IC:ComplexPortal.
DR GO; GO:0003682; F:chromatin binding; IDA:SGD.
DR GO; GO:0003688; F:DNA replication origin binding; IDA:SGD.
DR GO; GO:0006270; P:DNA replication initiation; IMP:SGD.
DR GO; GO:0006267; P:pre-replicative complex assembly involved in nuclear cell cycle DNA replication; IDA:SGD.
DR GO; GO:0030466; P:silent mating-type cassette heterochromatin assembly; IDA:SGD.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR020795; ORC3.
DR InterPro; IPR040855; ORC_WH_C.
DR PANTHER; PTHR12748; PTHR12748; 2.
DR Pfam; PF18137; ORC_WH_C; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Direct protein sequencing; DNA replication;
KW DNA-binding; Nucleus; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0007744|PubMed:22814378"
FT CHAIN 2..616
FT /note="Origin recognition complex subunit 3"
FT /id="PRO_0000127086"
FT MOD_RES 2
FT /note="N-acetylserine"
FT /evidence="ECO:0007744|PubMed:22814378"
FT STRAND 20..25
FT /evidence="ECO:0007829|PDB:5ZR1"
FT TURN 45..48
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 51..91
FT /evidence="ECO:0007829|PDB:5ZR1"
FT STRAND 103..108
FT /evidence="ECO:0007829|PDB:5ZR1"
FT STRAND 122..131
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 139..155
FT /evidence="ECO:0007829|PDB:5ZR1"
FT TURN 156..158
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 186..189
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 192..195
FT /evidence="ECO:0007829|PDB:5ZR1"
FT STRAND 199..206
FT /evidence="ECO:0007829|PDB:5ZR1"
FT TURN 208..210
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 213..224
FT /evidence="ECO:0007829|PDB:5ZR1"
FT STRAND 227..231
FT /evidence="ECO:0007829|PDB:5ZR1"
FT STRAND 234..238
FT /evidence="ECO:0007829|PDB:5ZR1"
FT TURN 245..250
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 253..256
FT /evidence="ECO:0007829|PDB:5ZR1"
FT TURN 257..260
FT /evidence="ECO:0007829|PDB:5ZR1"
FT STRAND 261..267
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 272..274
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 277..287
FT /evidence="ECO:0007829|PDB:5ZR1"
FT TURN 288..291
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 296..305
FT /evidence="ECO:0007829|PDB:5ZR1"
FT STRAND 310..312
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 314..331
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 333..338
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 340..345
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 348..354
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 358..370
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 375..380
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 387..392
FT /evidence="ECO:0007829|PDB:5ZR1"
FT STRAND 394..397
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 402..415
FT /evidence="ECO:0007829|PDB:5ZR1"
FT TURN 416..418
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 425..433
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 437..440
FT /evidence="ECO:0007829|PDB:5ZR1"
FT TURN 447..452
FT /evidence="ECO:0007829|PDB:5ZR1"
FT TURN 461..464
FT /evidence="ECO:0007829|PDB:5ZR1"
FT STRAND 465..468
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 475..478
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 483..492
FT /evidence="ECO:0007829|PDB:5ZR1"
FT TURN 495..497
FT /evidence="ECO:0007829|PDB:5ZR1"
FT TURN 504..507
FT /evidence="ECO:0007829|PDB:5ZR1"
FT STRAND 508..510
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 511..514
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 520..529
FT /evidence="ECO:0007829|PDB:5ZR1"
FT STRAND 533..536
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 537..545
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 550..558
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 564..571
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 575..584
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 587..593
FT /evidence="ECO:0007829|PDB:5ZR1"
FT TURN 602..604
FT /evidence="ECO:0007829|PDB:5ZR1"
FT STRAND 606..610
FT /evidence="ECO:0007829|PDB:5ZR1"
SQ SEQUENCE 616 AA; 72077 MW; 3FAAEE3F645D3F9C CRC64;
MSDLNQSKKM NVSEFADAQR SHYTVYPSLP QSNKNDKHIP FVKLLSGKES EVNVEKRWEL
YHQLHSHFHD QVDHIIDNIE ADLKAEISDL LYSETTQKRR CFNTIFLLGS DSTTKIELKD
ESSRYNVLIE LTPKESPNVR MMLRRSMYKL YSAADAEEHP TIKYEDINDE DGDFTEQNND
VSYDLSLVEN FKRLFGKDLA MVFNFKDVDS INFNTLDNFI ILLKSAFKYD HVKISLIFNI
NTNLSNIEKN LRQSTIRLLK RNYHKLDVSS NKGFKYGNQI FQSFLDTVDG KLNLSDRFVE
FILSKMANNT NHNLQLLTKM LDYSLMSYFF QNAFSVFIDP VNVDFLNDDY LKILSRCPTF
MFFVEGLIKQ HAPADEILSL LTNKNRGLEE FFVEFLVREN PINGHAKFVA RFLEEELNIT
NFNLIELYHN LLIGKLDSYL DRWSACKEYK DRLHFEPIDT IFQELFTLDN RSGLLTQSIF
PSYKSNIEDN LLSWEQVLPS LDKENYDTLS GDLDKIMAPV LGQLFKLYRE ANMTINIYDF
YIAFRETLPK EEILNFIRKD PSNTKLLELA ETPDAFDKVA LILFMQAIFA FENMGLIKFQ
STKSYDLVEK CVWRGI
