ORC4_ARATH
ID ORC4_ARATH Reviewed; 417 AA.
AC Q6EWX1; F4IM83; Q6E7G9; Q70BT4; Q9SJV4;
DT 07-JAN-2015, integrated into UniProtKB/Swiss-Prot.
DT 16-AUG-2004, sequence version 1.
DT 03-AUG-2022, entry version 91.
DE RecName: Full=Origin of replication complex subunit 4 {ECO:0000303|PubMed:16179646};
DE Short=AtORC4 {ECO:0000303|PubMed:16179646};
GN Name=ORC4 {ECO:0000303|PubMed:16179646};
GN OrderedLocusNames=At2g01120 {ECO:0000312|Araport:AT2G01120};
GN ORFNames=F23H14.9 {ECO:0000312|EMBL:AAF18660.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|EMBL:CAE01428.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), SUBUNIT, INTERACTION WITH ORC2 AND
RP ORC3, TISSUE SPECIFICITY, INDUCTION BY SUCROSE, AND GENE FAMILY.
RX PubMed=15358564; DOI=10.1016/j.febslet.2004.07.088;
RA Masuda H.P., Ramos G.B.A., de Almeida-Engler J., Cabral L.M.,
RA Coqueiro V.M., Macrini C.M.T., Ferreira P.C.G., Hemerly A.S.;
RT "Genome based identification and analysis of the pre-replicative complex of
RT Arabidopsis thaliana.";
RL FEBS Lett. 574:192-202(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC STRAIN=cv. Landsberg erecta; TISSUE=Flower;
RX PubMed=15020747; DOI=10.1105/tpc.019059;
RA Collinge M.A., Spillane C., Kohler C., Gheyselinck J., Grossniklaus U.;
RT "Genetic interaction of an origin recognition complex subunit and the
RT Polycomb group gene MEDEA during seed development.";
RL Plant Cell 16:1035-1046(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RA Diaz-Trivino S., Castellano M.M., Gutierrez C.;
RT "Organization of Arabidopsis origin recognition complex genes.";
RL Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [5]
RP GENOME REANNOTATION, AND SEQUENCE REVISION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 285-417 (ISOFORM 1).
RA Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP SUBUNIT, INTERACTION WITH ORC1A; ORC2; ORC3; ORC5 AND ORC6, TISSUE
RP SPECIFICITY, INDUCTION BY E2F AND SUCROSE, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16179646; DOI=10.1093/nar/gki854;
RA Diaz-Trivino S., Castellano M.M., Sanchez M.P., Ramirez-Parra E.,
RA Desvoyes B., Gutierrez C.;
RT "The genes encoding Arabidopsis ORC subunits are E2F targets and the two
RT ORC1 genes are differently expressed in proliferating and endoreplicating
RT cells.";
RL Nucleic Acids Res. 33:5404-5414(2005).
RN [8]
RP REVIEW ON THE CORE DNA REPLICATION MACHINERY.
RX PubMed=17556508; DOI=10.1104/pp.107.101105;
RA Shultz R.W., Tatineni V.M., Hanley-Bowdoin L., Thompson W.F.;
RT "Genome-wide analysis of the core DNA replication machinery in the higher
RT plants Arabidopsis and rice.";
RL Plant Physiol. 144:1697-1714(2007).
CC -!- FUNCTION: Component of the origin recognition complex (ORC) that binds
CC origins of replication. DNA-binding is ATP-dependent. The specific DNA
CC sequences that define origins of replication have not been identified
CC yet. ORC is required to assemble the pre-replication complex necessary
CC to initiate DNA replication. {ECO:0000250|UniProtKB:O43929}.
CC -!- SUBUNIT: Component of the origin recognition complex (ORC) composed of
CC at least ORC1 (ORC1A or ORC1B), ORC2, ORC3, ORC4, ORC5 and ORC6. ORC is
CC regulated in a cell-cycle and development dependent manner. It is
CC sequentially assembled at the exit from anaphase of mitosis and
CC disassembled as cells enter S phase. Interacts directly with ORC1A,
CC ORC2, ORC3, ORC5 and ORC6. {ECO:0000269|PubMed:15358564,
CC ECO:0000269|PubMed:16179646}.
CC -!- INTERACTION:
CC Q6EWX1; Q38899: ORC2; NbExp=2; IntAct=EBI-2114176, EBI-2114089;
CC Q6EWX1; Q6EWX0: ORC5; NbExp=2; IntAct=EBI-2114176, EBI-2114109;
CC Q6EWX1; Q9ZVH3: ORC6; NbExp=2; IntAct=EBI-2114176, EBI-2114077;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:O43929}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Comment=Additional isoforms seem to exist. {ECO:0000305};
CC Name=1; Synonyms=AtORC4a {ECO:0000303|PubMed:15358564};
CC IsoId=Q6EWX1-1; Sequence=Displayed;
CC Name=2; Synonyms=AtORC4b {ECO:0000303|PubMed:15358564};
CC IsoId=Q6EWX1-2; Sequence=VSP_057259, VSP_057260;
CC -!- TISSUE SPECIFICITY: Follow a cell-cycle regulation with a peak at the
CC G1/S-phase (PubMed:16179646). Isoform AtORC4a is expressed at low
CC levels ubiquitously (PubMed:15358564). Isoform AtORC4b is mostly
CC expressed in siliques, flowers and flower buds, and, to a lower exent,
CC in roots, leaves and stems (PubMed:16179646, PubMed:15358564).
CC {ECO:0000269|PubMed:15358564, ECO:0000269|PubMed:16179646}.
CC -!- INDUCTION: Regulated by E2F (PubMed:16179646). Accumulates rapidly
CC after cell cycle reactivation by sucrose addition following cell cycle
CC arrest mediated by sucrose deprivation (PubMed:16179646,
CC PubMed:15358564). {ECO:0000269|PubMed:15358564,
CC ECO:0000269|PubMed:16179646}.
CC -!- SIMILARITY: Belongs to the ORC4 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF18660.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAF18660.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=AAR92242.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=CAE52912.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AY524003; AAT37464.2; -; mRNA.
DR EMBL; AJ586913; CAE52912.1; ALT_FRAME; mRNA.
DR EMBL; AJ575582; CAE01428.1; -; mRNA.
DR EMBL; AC006837; AAF18660.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC05403.1; -; Genomic_DNA.
DR EMBL; BT011206; AAR92242.1; ALT_INIT; mRNA.
DR EMBL; BT011662; AAS47668.1; -; mRNA.
DR PIR; H84420; H84420.
DR RefSeq; NP_178222.4; NM_126174.5.
DR AlphaFoldDB; Q6EWX1; -.
DR SMR; Q6EWX1; -.
DR IntAct; Q6EWX1; 5.
DR STRING; 3702.AT2G01120.2; -.
DR PaxDb; Q6EWX1; -.
DR PRIDE; Q6EWX1; -.
DR ProteomicsDB; 248766; -. [Q6EWX1-1]
DR DNASU; 814641; -.
DR GeneID; 814641; -.
DR KEGG; ath:AT2G01120; -.
DR Araport; AT2G01120; -.
DR eggNOG; KOG2228; Eukaryota.
DR PhylomeDB; Q6EWX1; -.
DR PRO; PR:Q6EWX1; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q6EWX1; baseline and differential.
DR Genevisible; Q6EWX1; AT.
DR GO; GO:0005664; C:nuclear origin of replication recognition complex; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0003688; F:DNA replication origin binding; IBA:GO_Central.
DR GO; GO:0006270; P:DNA replication initiation; IBA:GO_Central.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003959; ATPase_AAA_core.
DR InterPro; IPR016527; ORC4.
DR InterPro; IPR032705; ORC4_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR12087; PTHR12087; 1.
DR Pfam; PF00004; AAA; 1.
DR Pfam; PF14629; ORC4_C; 1.
DR PIRSF; PIRSF007858; ORC4; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; ATP-binding; DNA replication; DNA-binding;
KW Nucleotide-binding; Nucleus; Reference proteome.
FT CHAIN 1..417
FT /note="Origin of replication complex subunit 4"
FT /id="PRO_0000431433"
FT BINDING 59..66
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT VAR_SEQ 303..322
FT /note="Missing (in isoform 2)"
FT /id="VSP_057259"
FT VAR_SEQ 361..374
FT /note="Missing (in isoform 2)"
FT /id="VSP_057260"
FT CONFLICT 72
FT /note="G -> V (in Ref. 2; CAE52912 and 1; AAT37464)"
FT /evidence="ECO:0000305"
FT CONFLICT 73
FT /note="V -> L (in Ref. 4; AAF18660)"
FT /evidence="ECO:0000305"
FT CONFLICT 106
FT /note="K -> R (in Ref. 2; CAE52912)"
FT /evidence="ECO:0000305"
FT CONFLICT 158..160
FT /note="QRL -> AAV (in Ref. 1; AAT37464)"
FT /evidence="ECO:0000305"
FT CONFLICT 165
FT /note="L -> LD (in Ref. 4; AAF18660 and 5; AEC05403)"
FT /evidence="ECO:0000305"
FT CONFLICT 262
FT /note="S -> T (in Ref. 2; CAE52912)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 417 AA; 47032 MW; 86F117ABDE1547EB CRC64;
MGKETPAEKS LNLIRGRLCD PSYVFRPLSA SSDSNYSKLK FIVSTSITEG CNNSMLLLGP
RGSGKAAVLD LGVGDLLEQF PDSVSVIRLN GLLHSDDNCA FKEIAKQLCM EHHLLFSKMA
SFDDNSQFII AMLRACGLAH KTIIFVLDEF DMFAQGKQRL LYSLLDAMQS VTSQAVVVGI
SSRLDADQLL EKRVRSRFSH RKFLFLPPSR EELDGLFVHL LSLPADSGFP SGYVSRFNDK
IKNLTSDTRF KDILKTLFNA NSTVNSFLKF IFCAVSLMNL ESGLLSLENF KAALSSMQRQ
PKLEAVRDCS VLELYLLVCM RRLEVKEQSS YNFISVMKEY KAIHDSFHTS DYYAQNVCLR
AFEHLRERQV ICYAENRGQS QTGEYRLQKL LISASELHQG MRSHACCPAI LLKLLDH
