ID   ORC4_RAT                Reviewed;         434 AA.
AC   Q6P9Z8;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 117.
DE   RecName: Full=Origin recognition complex subunit 4;
GN   Name=Orc4; Synonyms=Orc4l;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Prostate;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Component of the origin recognition complex (ORC) that binds
CC       origins of replication. DNA-binding is ATP-dependent. The specific DNA
CC       sequences that define origins of replication have not been identified
CC       yet. ORC is required to assemble the pre-replication complex necessary
CC       to initiate DNA replication. Binds histone H3 and H4 trimethylation
CC       marks H3K9me3, H3K27me3 and H4K20me3 (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of ORC, a complex composed of at least 6 subunits:
CC       ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6. ORC is regulated in a cell-cycle
CC       dependent manner. It is sequentially assembled at the exit from
CC       anaphase of mitosis and disassembled as cells enter S phase (By
CC       similarity). Interacts with DBF4 (By similarity). Interacts with POLQ
CC       (By similarity). {ECO:0000250|UniProtKB:O43929,
CC       ECO:0000250|UniProtKB:O88708}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ORC4 family. {ECO:0000305}.
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DR   EMBL; BC060516; AAH60516.1; -; mRNA.
DR   RefSeq; NP_954523.1; NM_199092.1.
DR   AlphaFoldDB; Q6P9Z8; -.
DR   SMR; Q6P9Z8; -.
DR   STRING; 10116.ENSRNOP00000006937; -.
DR   PaxDb; Q6P9Z8; -.
DR   GeneID; 295596; -.
DR   KEGG; rno:295596; -.
DR   UCSC; RGD:735212; rat.
DR   CTD; 5000; -.
DR   RGD; 735212; Orc4.
DR   VEuPathDB; HostDB:ENSRNOG00000005021; -.
DR   eggNOG; KOG2228; Eukaryota.
DR   HOGENOM; CLU_007115_0_1_1; -.
DR   InParanoid; Q6P9Z8; -.
DR   OMA; KVSTTMQ; -.
DR   OrthoDB; 1543398at2759; -.
DR   PhylomeDB; Q6P9Z8; -.
DR   TreeFam; TF101094; -.
DR   Reactome; R-RNO-176187; Activation of ATR in response to replication stress.
DR   Reactome; R-RNO-68616; Assembly of the ORC complex at the origin of replication.
DR   Reactome; R-RNO-68689; CDC6 association with the ORC:origin complex.
DR   Reactome; R-RNO-68949; Orc1 removal from chromatin.
DR   Reactome; R-RNO-68962; Activation of the pre-replicative complex.
DR   PRO; PR:Q6P9Z8; -.
DR   Proteomes; UP000002494; Chromosome 3.
DR   Bgee; ENSRNOG00000005021; Expressed in testis and 19 other tissues.
DR   Genevisible; Q6P9Z8; RN.
DR   GO; GO:0005664; C:nuclear origin of replication recognition complex; ISS:UniProtKB.
DR   GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR   GO; GO:0000808; C:origin recognition complex; ISO:RGD.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003688; F:DNA replication origin binding; ISS:UniProtKB.
DR   GO; GO:0000166; F:nucleotide binding; ISS:UniProtKB.
DR   GO; GO:0006270; P:DNA replication initiation; ISS:UniProtKB.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR041664; AAA_16.
DR   InterPro; IPR016527; ORC4.
DR   InterPro; IPR032705; ORC4_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR12087; PTHR12087; 1.
DR   Pfam; PF13191; AAA_16; 1.
DR   Pfam; PF14629; ORC4_C; 1.
DR   PIRSF; PIRSF007858; ORC4; 1.
DR   SMART; SM00382; AAA; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; DNA replication; DNA-binding; Methylation; Nucleotide-binding;
KW   Nucleus; Reference proteome.
FT   CHAIN           1..434
FT                   /note="Origin recognition complex subunit 4"
FT                   /id="PRO_0000328611"
FT   BINDING         65..72
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   MOD_RES         7
FT                   /note="N6-methyllysine"
FT                   /evidence="ECO:0000250|UniProtKB:O43929"
SQ   SEQUENCE   434 AA;  50105 MW;  2AF6A68ACF871852 CRC64;
     MSNRKSKNNI HAECLSQVQR ILRERFCHHT PHSNLFGVQV QYKHLIELLK RTAIYGESNS
     LLIVGPRGSG KTTLLNHALK ELMQIGDMSE NVLQVHLNGL LQTNDKIALK EITRQLNLEN
     VVGDKVFGSF AENLSFLLEA LQKGNRTSSC PVIFILDEFD LFAHQKNQTL LYNLFDISQS
     AQTPIAVIGL TCRLDILELL EKRVKSRFSH RQIHLMNSFD FPQYLKIFKE QLSLPAEFPD
     KIFAEKWNEN AHCLSEDSTV LEVLQKHFNV NKNLQSLHML LMLALNRVTV THPFMTSADL
     MEAQHLCSLD AKASIVHGLS VLEICLIIAM KHLNDIYEEE PFNFQMVYNE FQKFIQRKAH
     SVYNFEKPVV MKAFEHLQQL ELIKPMERTS VNSQREYQLV KLLLDNTQIM NALQKYSNCP
     TDVRQWATSS LSWL