ORC4_YEAST
ID ORC4_YEAST Reviewed; 529 AA.
AC P54791; D6W4G3;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 175.
DE RecName: Full=Origin recognition complex subunit 4;
DE AltName: Full=Origin recognition complex 56 kDa subunit;
GN Name=ORC4; OrderedLocusNames=YPR162C; ORFNames=P9325.5;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RX PubMed=7585959; DOI=10.1016/0092-8674(95)90096-9;
RA Bell S.P., Mitchell J., Leber J., Kobayashi R., Stillman B.;
RT "The multidomain structure of Orc1p reveals similarity to regulators of DNA
RT replication and transcriptional silencing.";
RL Cell 83:563-568(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169875;
RA Bussey H., Storms R.K., Ahmed A., Albermann K., Allen E., Ansorge W.,
RA Araujo R., Aparicio A., Barrell B.G., Badcock K., Benes V., Botstein D.,
RA Bowman S., Brueckner M., Carpenter J., Cherry J.M., Chung E.,
RA Churcher C.M., Coster F., Davis K., Davis R.W., Dietrich F.S., Delius H.,
RA DiPaolo T., Dubois E., Duesterhoeft A., Duncan M., Floeth M., Fortin N.,
RA Friesen J.D., Fritz C., Goffeau A., Hall J., Hebling U., Heumann K.,
RA Hilbert H., Hillier L.W., Hunicke-Smith S., Hyman R.W., Johnston M.,
RA Kalman S., Kleine K., Komp C., Kurdi O., Lashkari D., Lew H., Lin A.,
RA Lin D., Louis E.J., Marathe R., Messenguy F., Mewes H.-W., Mirtipati S.,
RA Moestl D., Mueller-Auer S., Namath A., Nentwich U., Oefner P., Pearson D.,
RA Petel F.X., Pohl T.M., Purnelle B., Rajandream M.A., Rechmann S.,
RA Rieger M., Riles L., Roberts D., Schaefer M., Scharfe M., Scherens B.,
RA Schramm S., Schroeder M., Sdicu A.-M., Tettelin H., Urrestarazu L.A.,
RA Ushinsky S., Vierendeels F., Vissers S., Voss H., Walsh S.V., Wambutt R.,
RA Wang Y., Wedler E., Wedler H., Winnett E., Zhong W.-W., Zollner A.,
RA Vo D.H., Hani J.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XVI.";
RL Nature 387:103-105(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-9, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
CC -!- FUNCTION: Component of the origin recognition complex (ORC) that binds
CC origins of replication. It has a role in both chromosomal replication
CC and mating type transcriptional silencing. Binds to the ARS consensus
CC sequence (ACS) of origins of replication.
CC -!- SUBUNIT: Component of the origin recognition complex (ORC) composed of
CC at least ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6.
CC -!- INTERACTION:
CC P54791; P54784: ORC1; NbExp=6; IntAct=EBI-12580, EBI-12568;
CC P54791; P54790: ORC3; NbExp=3; IntAct=EBI-12580, EBI-12576;
CC P54791; P50874: ORC5; NbExp=9; IntAct=EBI-12580, EBI-12584;
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- MISCELLANEOUS: Present with 2170 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the ORC4 family. {ECO:0000305}.
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DR EMBL; U34862; AAB38250.1; -; Genomic_DNA.
DR EMBL; U25840; AAB68149.1; -; Genomic_DNA.
DR EMBL; AY723871; AAU09788.1; -; Genomic_DNA.
DR EMBL; BK006949; DAA11579.1; -; Genomic_DNA.
DR PIR; S59821; S59821.
DR RefSeq; NP_015488.1; NM_001184259.1.
DR PDB; 5V8F; EM; 3.90 A; D=1-529.
DR PDB; 5ZR1; EM; 3.00 A; D=1-529.
DR PDB; 6RQC; EM; 4.40 A; D=1-529.
DR PDB; 6WGC; EM; 4.30 A; D=1-529.
DR PDB; 6WGG; EM; 8.10 A; D=1-529.
DR PDB; 6WGI; EM; 10.00 A; D=1-529.
DR PDB; 7MCA; EM; 3.60 A; D=1-529.
DR PDBsum; 5V8F; -.
DR PDBsum; 5ZR1; -.
DR PDBsum; 6RQC; -.
DR PDBsum; 6WGC; -.
DR PDBsum; 6WGG; -.
DR PDBsum; 6WGI; -.
DR PDBsum; 7MCA; -.
DR AlphaFoldDB; P54791; -.
DR SMR; P54791; -.
DR BioGRID; 36335; 332.
DR ComplexPortal; CPX-768; Nuclear origin recognition complex.
DR DIP; DIP-2287N; -.
DR IntAct; P54791; 17.
DR MINT; P54791; -.
DR STRING; 4932.YPR162C; -.
DR iPTMnet; P54791; -.
DR MaxQB; P54791; -.
DR PaxDb; P54791; -.
DR PRIDE; P54791; -.
DR EnsemblFungi; YPR162C_mRNA; YPR162C; YPR162C.
DR GeneID; 856291; -.
DR KEGG; sce:YPR162C; -.
DR SGD; S000006366; ORC4.
DR VEuPathDB; FungiDB:YPR162C; -.
DR eggNOG; KOG2228; Eukaryota.
DR GeneTree; ENSGT00390000016542; -.
DR HOGENOM; CLU_007115_4_0_1; -.
DR InParanoid; P54791; -.
DR OMA; QRIIYMP; -.
DR BioCyc; YEAST:G3O-34291-MON; -.
DR Reactome; R-SCE-68616; Assembly of the ORC complex at the origin of replication.
DR Reactome; R-SCE-68689; CDC6 association with the ORC:origin complex.
DR Reactome; R-SCE-68949; Orc1 removal from chromatin.
DR Reactome; R-SCE-68962; Activation of the pre-replicative complex.
DR PRO; PR:P54791; -.
DR Proteomes; UP000002311; Chromosome XVI.
DR RNAct; P54791; protein.
DR GO; GO:0031261; C:DNA replication preinitiation complex; IDA:SGD.
DR GO; GO:0005664; C:nuclear origin of replication recognition complex; IDA:SGD.
DR GO; GO:0005656; C:nuclear pre-replicative complex; IDA:SGD.
DR GO; GO:0005654; C:nucleoplasm; TAS:Reactome.
DR GO; GO:0005634; C:nucleus; IC:ComplexPortal.
DR GO; GO:0003688; F:DNA replication origin binding; IDA:SGD.
DR GO; GO:0006270; P:DNA replication initiation; IMP:SGD.
DR GO; GO:0006267; P:pre-replicative complex assembly involved in nuclear cell cycle DNA replication; IDA:SGD.
DR GO; GO:0030466; P:silent mating-type cassette heterochromatin assembly; IDA:SGD.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR016527; ORC4.
DR InterPro; IPR032705; ORC4_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR12087; PTHR12087; 1.
DR Pfam; PF14629; ORC4_C; 1.
DR PIRSF; PIRSF007858; ORC4; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; DNA replication; DNA-binding;
KW Nucleus; Phosphoprotein; Reference proteome.
FT CHAIN 1..529
FT /note="Origin recognition complex subunit 4"
FT /id="PRO_0000127091"
FT MOD_RES 9
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956"
FT HELIX 47..58
FT /evidence="ECO:0007829|PDB:5ZR1"
FT TURN 59..62
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 73..75
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 76..87
FT /evidence="ECO:0007829|PDB:5ZR1"
FT TURN 88..91
FT /evidence="ECO:0007829|PDB:5ZR1"
FT STRAND 97..101
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 108..120
FT /evidence="ECO:0007829|PDB:5ZR1"
FT STRAND 127..133
FT /evidence="ECO:0007829|PDB:5ZR1"
FT TURN 134..136
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 141..157
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 177..188
FT /evidence="ECO:0007829|PDB:5ZR1"
FT STRAND 211..218
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 219..221
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 230..240
FT /evidence="ECO:0007829|PDB:5ZR1"
FT STRAND 245..251
FT /evidence="ECO:0007829|PDB:5ZR1"
FT TURN 257..259
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 262..267
FT /evidence="ECO:0007829|PDB:5ZR1"
FT STRAND 272..274
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 281..292
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 302..314
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 319..330
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 334..346
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 351..360
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 362..370
FT /evidence="ECO:0007829|PDB:5ZR1"
FT STRAND 373..376
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 377..380
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 385..400
FT /evidence="ECO:0007829|PDB:5ZR1"
FT STRAND 401..404
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 408..425
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 453..465
FT /evidence="ECO:0007829|PDB:5ZR1"
FT STRAND 468..471
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 481..489
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 493..496
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 499..501
FT /evidence="ECO:0007829|PDB:5ZR1"
FT STRAND 504..508
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 510..516
FT /evidence="ECO:0007829|PDB:5ZR1"
FT HELIX 524..526
FT /evidence="ECO:0007829|PDB:5ZR1"
SQ SEQUENCE 529 AA; 60705 MW; ACF064F2C633F13F CRC64;
MTISEARLSP QVNLLPIKRH SNEEVEETAA ILKKRTIDNE KCKDSDPGFG SLQRRLLQQL
YGTLPTDEKI IFTYLQDCQQ EIDRIIKQSI IQKESHSVIL VGPRQSYKTY LLDYELSLLQ
QSYKEQFITI RLNGFIHSEQ TAINGIATQL EQQLQKIHGS EEKIDDTSLE TISSGSLTEV
FEKILLLLDS TTKTRNEDSG EVDRESITKI TVVFIFDEID TFAGPVRQTL LYNLFDMVEH
SRVPVCIFGC TTKLNILEYL EKRVKSRFSQ RVIYMPQIQN LDDMVDAVRN LLTVRSEISP
WVSQWNETLE KELSDPRSNL NRHIRMNFET FRSLPTLKNS IIPLVATSKN FGSLCTAIKS
CSFLDIYNKN QLSNNLTGRL QSLSDLELAI LISAARVALR AKDGSFNFNL AYAEYEKMIK
AINSRIPTVA PTTNVGTGQS TFSIDNTIKL WLKKDVKNVW ENLVQLDFFT EKSAVGLRDN
ATAAFYASNY QFQGTMIPFD LRSYQMQIIL QELRRIIPKS NMYYSWTQL
