ORC5_ARATH
ID ORC5_ARATH Reviewed; 534 AA.
AC Q6EWX0; Q9SZR2;
DT 07-JAN-2015, integrated into UniProtKB/Swiss-Prot.
DT 16-AUG-2004, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Origin of replication complex subunit 5 {ECO:0000303|PubMed:16179646};
DE Short=AtORC5 {ECO:0000303|PubMed:16179646};
GN Name=ORC5 {ECO:0000303|PubMed:16179646};
GN OrderedLocusNames=At4g29910 {ECO:0000312|Araport:AT4G29910};
GN ORFNames=F27B13.150 {ECO:0000312|EMBL:CAB43666.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|EMBL:CAE01429.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Diaz-Trivino S., Castellano M.M., Gutierrez C.;
RT "Organization of Arabidopsis origin recognition complex genes.";
RL Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP SUBUNIT, INTERACTION WITH ORC3, TISSUE SPECIFICITY, INDUCTION BY SUCROSE,
RP AND GENE FAMILY.
RC STRAIN=cv. Columbia;
RX PubMed=15358564; DOI=10.1016/j.febslet.2004.07.088;
RA Masuda H.P., Ramos G.B.A., de Almeida-Engler J., Cabral L.M.,
RA Coqueiro V.M., Macrini C.M.T., Ferreira P.C.G., Hemerly A.S.;
RT "Genome based identification and analysis of the pre-replicative complex of
RT Arabidopsis thaliana.";
RL FEBS Lett. 574:192-202(2004).
RN [6]
RP SUBUNIT, INTERACTION WITH ORC1A; ORC1B; ORC2; ORC3; ORC4 AND ORC6, TISSUE
RP SPECIFICITY, INDUCTION BY SUCROSE, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=16179646; DOI=10.1093/nar/gki854;
RA Diaz-Trivino S., Castellano M.M., Sanchez M.P., Ramirez-Parra E.,
RA Desvoyes B., Gutierrez C.;
RT "The genes encoding Arabidopsis ORC subunits are E2F targets and the two
RT ORC1 genes are differently expressed in proliferating and endoreplicating
RT cells.";
RL Nucleic Acids Res. 33:5404-5414(2005).
RN [7]
RP REVIEW ON THE CORE DNA REPLICATION MACHINERY.
RX PubMed=17556508; DOI=10.1104/pp.107.101105;
RA Shultz R.W., Tatineni V.M., Hanley-Bowdoin L., Thompson W.F.;
RT "Genome-wide analysis of the core DNA replication machinery in the higher
RT plants Arabidopsis and rice.";
RL Plant Physiol. 144:1697-1714(2007).
CC -!- FUNCTION: Component of the origin recognition complex (ORC) that binds
CC origins of replication. DNA-binding is ATP-dependent. The specific DNA
CC sequences that define origins of replication have not been identified
CC yet. ORC is required to assemble the pre-replication complex necessary
CC to initiate DNA replication. {ECO:0000250|UniProtKB:O43913}.
CC -!- SUBUNIT: Component of the origin recognition complex (ORC) composed of
CC at least ORC1 (ORC1A or ORC1B), ORC2, ORC3, ORC4, ORC5 and ORC6. ORC is
CC regulated in a cell-cycle and development dependent manner. It is
CC sequentially assembled at the exit from anaphase of mitosis and
CC disassembled as cells enter S phase. Interacts directly with ORC1A,
CC ORC1B, ORC2, ORC3, ORC4 and ORC6. {ECO:0000269|PubMed:15358564,
CC ECO:0000269|PubMed:16179646}.
CC -!- INTERACTION:
CC Q6EWX0; Q38899: ORC2; NbExp=2; IntAct=EBI-2114109, EBI-2114089;
CC Q6EWX0; Q6EWX1: ORC4; NbExp=2; IntAct=EBI-2114109, EBI-2114176;
CC Q6EWX0; Q9ZVH3: ORC6; NbExp=2; IntAct=EBI-2114109, EBI-2114077;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:O43913}.
CC -!- TISSUE SPECIFICITY: Follow a cell-cycle regulation with a peak at the
CC G1/S-phase (PubMed:16179646). Mostly expressed in flower buds and
CC cauline leaves, and, to a lower exent, in roots, leaves and stems
CC (PubMed:16179646). Expressed at low levels ubiquitously
CC (PubMed:15358564). {ECO:0000269|PubMed:15358564,
CC ECO:0000269|PubMed:16179646}.
CC -!- INDUCTION: Accumulates 13 hours after cell cycle reactivation by
CC sucrose addition following cell cycle arrest mediated by sucrose
CC deprivation. {ECO:0000269|PubMed:15358564,
CC ECO:0000269|PubMed:16179646}.
CC -!- SIMILARITY: Belongs to the ORC5 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB43666.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB79749.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AJ575583; CAE01429.1; -; mRNA.
DR EMBL; AL050352; CAB43666.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161575; CAB79749.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE85692.1; -; Genomic_DNA.
DR EMBL; AK226860; BAE98949.1; -; mRNA.
DR PIR; T08552; T08552.
DR RefSeq; NP_194720.2; NM_119137.5.
DR AlphaFoldDB; Q6EWX0; -.
DR SMR; Q6EWX0; -.
DR IntAct; Q6EWX0; 5.
DR STRING; 3702.AT4G29910.1; -.
DR iPTMnet; Q6EWX0; -.
DR PaxDb; Q6EWX0; -.
DR PRIDE; Q6EWX0; -.
DR ProteomicsDB; 248771; -.
DR EnsemblPlants; AT4G29910.1; AT4G29910.1; AT4G29910.
DR GeneID; 829114; -.
DR Gramene; AT4G29910.1; AT4G29910.1; AT4G29910.
DR KEGG; ath:AT4G29910; -.
DR Araport; AT4G29910; -.
DR TAIR; locus:2123934; AT4G29910.
DR eggNOG; KOG2543; Eukaryota.
DR HOGENOM; CLU_028223_1_0_1; -.
DR InParanoid; Q6EWX0; -.
DR OMA; QLRRWHG; -.
DR PhylomeDB; Q6EWX0; -.
DR PRO; PR:Q6EWX0; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q6EWX0; baseline and differential.
DR Genevisible; Q6EWX0; AT.
DR GO; GO:0005664; C:nuclear origin of replication recognition complex; IBA:GO_Central.
DR GO; GO:0000808; C:origin recognition complex; ISS:TAIR.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003688; F:DNA replication origin binding; IBA:GO_Central.
DR GO; GO:0006260; P:DNA replication; ISS:TAIR.
DR GO; GO:0006270; P:DNA replication initiation; IBA:GO_Central.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR020796; ORC5.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR12705; PTHR12705; 1.
DR Pfam; PF14630; ORC5_C; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 1: Evidence at protein level;
KW ATP-binding; DNA replication; Nucleotide-binding; Nucleus;
KW Reference proteome.
FT CHAIN 1..534
FT /note="Origin of replication complex subunit 5"
FT /id="PRO_0000431435"
FT REGION 1..36
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 397..428
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 129..136
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
FT COMPBIAS 13..33
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 403..427
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 83..90
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
SQ SEQUENCE 534 AA; 60638 MW; 8DA66E2E9577403C CRC64;
MPPKEESSKV TRRSTRSSAS VTVENSEPIE SHTPTIDDLT FGEESITIDA VLSNFPGRRS
QIFDFIRLMG PLDCPTLPIM IYGGASTGKT SVVLQVLRHL NRPFVYSSCR TCYNPRILFE
SILNQSLLHR KCSLNGYSSA KRCDKPSDFV NLLREALSSV IKTLESTSET SRSDKPDEKP
MGKMVYLILD NVDLIRDWDK GTIILQFLFS LYTVLKMPQL GIILISGLPP DVYYSNMGYT
DPIPLYFPEY SEEDLRQIFL RNQPNRKLYS AFLDVVLKPF CRVTRRVEEL STTFSLLFRK
FCEPLDDLGI SPNEDMKRRL YSHLKPLIAH CLNEIFRVSS HPHDGETRGE RRQKASYSSE
NREELEILDF HMSTSAKFLL LSAFLASRNP ATLDASMFDS TGGMDNRKRK RKASEKSMEK
KEIAEQEAVM KGPGSFPLER LLAIFQCIAS VGDSSFGEED EEEENTTGYD KENNNLMSDI
LLQVSSLCDA NFLIKSGSCP LEGSIRYRSM VSEDLAQKVA KSLSFPLSKY LYRR
