ID   ORC5_DICDI              Reviewed;         571 AA.
AC   Q54C22;
DT   29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   03-AUG-2022, entry version 77.
DE   RecName: Full=Origin recognition complex subunit 5;
DE   AltName: Full=Origin replication complex subunit E;
GN   Name=orcE; Synonyms=orc5; ORFNames=DDB_G0293214;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: Component of the origin recognition complex (ORC) that binds
CC       origins of replication. DNA-binding is ATP-dependent, however specific
CC       DNA sequences that define origins of replication have not been
CC       identified so far. ORC is required to assemble the pre-replication
CC       complex necessary to initiate DNA replication (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: ORC is composed of six subunits. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the ORC1 family. {ECO:0000305}.
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DR   EMBL; AAFI02000200; EAL60806.1; -; Genomic_DNA.
DR   RefSeq; XP_629244.1; XM_629242.1.
DR   AlphaFoldDB; Q54C22; -.
DR   SMR; Q54C22; -.
DR   STRING; 44689.DDB0233112; -.
DR   PaxDb; Q54C22; -.
DR   PRIDE; Q54C22; -.
DR   EnsemblProtists; EAL60806; EAL60806; DDB_G0293214.
DR   GeneID; 8629126; -.
DR   KEGG; ddi:DDB_G0293214; -.
DR   dictyBase; DDB_G0293214; orcE.
DR   eggNOG; KOG2543; Eukaryota.
DR   HOGENOM; CLU_477720_0_0_1; -.
DR   InParanoid; Q54C22; -.
DR   OMA; IMMSIST; -.
DR   PhylomeDB; Q54C22; -.
DR   Reactome; R-DDI-68616; Assembly of the ORC complex at the origin of replication.
DR   Reactome; R-DDI-68689; CDC6 association with the ORC:origin complex.
DR   Reactome; R-DDI-68949; Orc1 removal from chromatin.
DR   Reactome; R-DDI-68962; Activation of the pre-replicative complex.
DR   PRO; PR:Q54C22; -.
DR   Proteomes; UP000002195; Chromosome 6.
DR   GO; GO:0005664; C:nuclear origin of replication recognition complex; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; ISS:dictyBase.
DR   GO; GO:0003688; F:DNA replication origin binding; IBA:GO_Central.
DR   GO; GO:0006270; P:DNA replication initiation; IBA:GO_Central.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR020796; ORC5.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR12705; PTHR12705; 1.
DR   Pfam; PF14630; ORC5_C; 1.
PE   3: Inferred from homology;
KW   DNA replication; DNA-binding; Nucleus; Reference proteome.
FT   CHAIN           1..571
FT                   /note="Origin recognition complex subunit 5"
FT                   /id="PRO_0000330358"
FT   REGION          90..142
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          404..430
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        105..134
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        416..430
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   571 AA;  66853 MW;  FE973750A61313C5 CRC64;
     MIETSKRSRI INKINNVVIS DYKGAIFVWG PKSTGKYTTI KHVLESNSRI YCEADCNEHY
     STASLFRSLL STLRNINKFG RRKPINYDDD DEYINNDINK DNEEDNNNNN NNNNNNNNNN
     NNNNNNNNNN NNDGYDDGYD DDYDYKDKSL NERIELLKIN DLSKEFNNMI IKSQIYSKKI
     PATSIPSVSE MVQVLYEKAS TNETISIILR HSEKLMKFEG EFFYSLFKIE EFTDHPVCLY
     LLSEIPTERI LSTGKEFKPI RNIHFPQYEP NEICSIVLNF KPNFSIINYN QNNNDSNNSN
     NNNNNNNHLK LKEEDNIKLY NKLVELVVRV FHTTTRDIID IIFVCHNLFP NYIEPIYNGT
     CGVEDSDSIL YQNIESKIVK SLRNITEKDE IVLEDQKRQQ ETYQIYPPQQ VPQQQKQQEK
     EKEKEKGRQL ASYSDAKNIP YHMKIVLISC FLASSFSRNK DKLLYTKEAV KRQSLSNEKT
     TGSKWFHFQR AREIGYKLFP SEKKALFQES IFKSLASLKY LETISSIEPA FTYKFKCTRR
     VHLEFIKQIS ASINFKIDTY LEAAQFQIIS K