ORC5_DROME
ID ORC5_DROME Reviewed; 460 AA.
AC Q24169; Q9V398;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=Origin recognition complex subunit 5;
GN Name=Orc5; ORFNames=CG7833;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=7502079; DOI=10.1126/science.270.5242.1674;
RA Gossen M., Pak D.T.S., Hansen S.K., Acharya J.K., Botchan M.R.;
RT "A Drosophila homolog of the yeast origin recognition complex.";
RL Science 270:1674-1677(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10471707; DOI=10.1093/genetics/153.1.179;
RA Ashburner M., Misra S., Roote J., Lewis S.E., Blazej R.G., Davis T.,
RA Doyle C., Galle R.F., George R.A., Harris N.L., Hartzell G., Harvey D.A.,
RA Hong L., Houston K.A., Hoskins R.A., Johnson G., Martin C., Moshrefi A.R.,
RA Palazzolo M., Reese M.G., Spradling A.C., Tsang G., Wan K.H., Whitelaw K.,
RA Celniker S.E., Rubin G.M.;
RT "An exploration of the sequence of a 2.9-Mb region of the genome of
RT Drosophila melanogaster: the Adh region.";
RL Genetics 153:179-219(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: Component of the origin recognition complex (ORC) that binds
CC origins of replication. DNA-binding is ATP-dependent, however specific
CC DNA sequences that define origins of replication have not been
CC identified so far. ORC is required to assemble the pre-replication
CC complex necessary to initiate DNA replication (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: ORC is composed of six subunits.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- SIMILARITY: Belongs to the ORC5 family. {ECO:0000305}.
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DR EMBL; U43505; AAC46956.1; -; mRNA.
DR EMBL; AE014134; AAF53340.1; -; Genomic_DNA.
DR EMBL; AY089590; AAL90328.1; -; mRNA.
DR RefSeq; NP_477132.1; NM_057784.8.
DR PDB; 4XGC; X-ray; 3.50 A; E=1-460.
DR PDB; 7JGR; EM; 3.90 A; E=1-460.
DR PDB; 7JGS; EM; 3.20 A; E=1-460.
DR PDB; 7JK2; EM; 3.20 A; E=1-460.
DR PDB; 7JK3; EM; 3.40 A; E=1-460.
DR PDB; 7JK4; EM; 3.40 A; E=1-460.
DR PDB; 7JK5; EM; 3.90 A; E=1-460.
DR PDB; 7JK6; EM; 4.00 A; E=1-460.
DR PDBsum; 4XGC; -.
DR PDBsum; 7JGR; -.
DR PDBsum; 7JGS; -.
DR PDBsum; 7JK2; -.
DR PDBsum; 7JK3; -.
DR PDBsum; 7JK4; -.
DR PDBsum; 7JK5; -.
DR PDBsum; 7JK6; -.
DR AlphaFoldDB; Q24169; -.
DR SMR; Q24169; -.
DR BioGRID; 60827; 13.
DR DIP; DIP-49320N; -.
DR IntAct; Q24169; 4.
DR STRING; 7227.FBpp0080120; -.
DR PaxDb; Q24169; -.
DR DNASU; 34794; -.
DR EnsemblMetazoa; FBtr0080543; FBpp0080120; FBgn0015271.
DR GeneID; 34794; -.
DR KEGG; dme:Dmel_CG7833; -.
DR CTD; 5001; -.
DR FlyBase; FBgn0015271; Orc5.
DR VEuPathDB; VectorBase:FBgn0015271; -.
DR eggNOG; KOG2543; Eukaryota.
DR GeneTree; ENSGT00390000009380; -.
DR HOGENOM; CLU_028223_0_1_1; -.
DR InParanoid; Q24169; -.
DR OMA; QLRRWHG; -.
DR OrthoDB; 571556at2759; -.
DR PhylomeDB; Q24169; -.
DR Reactome; R-DME-176187; Activation of ATR in response to replication stress.
DR Reactome; R-DME-68616; Assembly of the ORC complex at the origin of replication.
DR Reactome; R-DME-68689; CDC6 association with the ORC:origin complex.
DR Reactome; R-DME-68949; Orc1 removal from chromatin.
DR Reactome; R-DME-68962; Activation of the pre-replicative complex.
DR SignaLink; Q24169; -.
DR BioGRID-ORCS; 34794; 1 hit in 3 CRISPR screens.
DR GenomeRNAi; 34794; -.
DR PRO; PR:Q24169; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0015271; Expressed in secondary oocyte and 34 other tissues.
DR Genevisible; Q24169; DM.
DR GO; GO:0005664; C:nuclear origin of replication recognition complex; IDA:FlyBase.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003688; F:DNA replication origin binding; IBA:GO_Central.
DR GO; GO:0043021; F:ribonucleoprotein complex binding; IDA:UniProtKB.
DR GO; GO:0006260; P:DNA replication; IMP:FlyBase.
DR GO; GO:0006270; P:DNA replication initiation; IDA:FlyBase.
DR GO; GO:0006261; P:DNA-templated DNA replication; ISS:FlyBase.
DR GO; GO:0007076; P:mitotic chromosome condensation; IMP:FlyBase.
DR GO; GO:0007052; P:mitotic spindle organization; IMP:FlyBase.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR041664; AAA_16.
DR InterPro; IPR020796; ORC5.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR12705; PTHR12705; 1.
DR Pfam; PF13191; AAA_16; 1.
DR Pfam; PF14630; ORC5_C; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; DNA replication; Nucleotide-binding; Nucleus;
KW Reference proteome.
FT CHAIN 1..460
FT /note="Origin recognition complex subunit 5"
FT /id="PRO_0000127094"
FT BINDING 41..48
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT HELIX 2..11
FT /evidence="ECO:0007829|PDB:7JGS"
FT HELIX 16..26
FT /evidence="ECO:0007829|PDB:7JGS"
FT STRAND 29..31
FT /evidence="ECO:0007829|PDB:7JGS"
FT STRAND 35..41
FT /evidence="ECO:0007829|PDB:7JGS"
FT STRAND 45..47
FT /evidence="ECO:0007829|PDB:7JK2"
FT HELIX 48..61
FT /evidence="ECO:0007829|PDB:7JGS"
FT STRAND 62..70
FT /evidence="ECO:0007829|PDB:7JGS"
FT TURN 71..73
FT /evidence="ECO:0007829|PDB:7JGS"
FT HELIX 77..88
FT /evidence="ECO:0007829|PDB:7JGS"
FT HELIX 93..95
FT /evidence="ECO:0007829|PDB:7JGS"
FT HELIX 101..109
FT /evidence="ECO:0007829|PDB:7JGS"
FT STRAND 115..117
FT /evidence="ECO:0007829|PDB:7JK3"
FT STRAND 120..127
FT /evidence="ECO:0007829|PDB:7JGS"
FT HELIX 128..133
FT /evidence="ECO:0007829|PDB:7JGS"
FT STRAND 136..138
FT /evidence="ECO:0007829|PDB:7JGS"
FT HELIX 139..142
FT /evidence="ECO:0007829|PDB:7JGS"
FT HELIX 145..149
FT /evidence="ECO:0007829|PDB:7JGS"
FT STRAND 151..161
FT /evidence="ECO:0007829|PDB:7JGS"
FT HELIX 163..165
FT /evidence="ECO:0007829|PDB:7JGS"
FT STRAND 176..180
FT /evidence="ECO:0007829|PDB:7JGS"
FT HELIX 185..193
FT /evidence="ECO:0007829|PDB:7JGS"
FT HELIX 196..205
FT /evidence="ECO:0007829|PDB:7JGS"
FT HELIX 212..219
FT /evidence="ECO:0007829|PDB:7JGS"
FT HELIX 223..237
FT /evidence="ECO:0007829|PDB:7JGS"
FT TURN 238..240
FT /evidence="ECO:0007829|PDB:7JGS"
FT HELIX 244..258
FT /evidence="ECO:0007829|PDB:7JGS"
FT HELIX 260..264
FT /evidence="ECO:0007829|PDB:7JGS"
FT HELIX 276..287
FT /evidence="ECO:0007829|PDB:7JGS"
FT TURN 288..291
FT /evidence="ECO:0007829|PDB:7JGS"
FT HELIX 323..337
FT /evidence="ECO:0007829|PDB:7JGS"
FT TURN 340..342
FT /evidence="ECO:0007829|PDB:7JGS"
FT HELIX 343..347
FT /evidence="ECO:0007829|PDB:7JGS"
FT TURN 373..375
FT /evidence="ECO:0007829|PDB:4XGC"
FT STRAND 379..381
FT /evidence="ECO:0007829|PDB:4XGC"
FT HELIX 382..393
FT /evidence="ECO:0007829|PDB:7JGS"
FT HELIX 401..412
FT /evidence="ECO:0007829|PDB:7JGS"
FT STRAND 415..420
FT /evidence="ECO:0007829|PDB:7JGS"
FT STRAND 422..424
FT /evidence="ECO:0007829|PDB:7JK2"
FT TURN 425..428
FT /evidence="ECO:0007829|PDB:7JGS"
FT STRAND 431..434
FT /evidence="ECO:0007829|PDB:7JGS"
FT HELIX 438..448
FT /evidence="ECO:0007829|PDB:7JGS"
FT TURN 453..455
FT /evidence="ECO:0007829|PDB:7JGS"
SQ SEQUENCE 460 AA; 52115 MW; FDCE3969E1CBF7D2 CRC64;
MEAICSSLEP LFPCREAAIE TLGELIGDSS ETYPSAIYLF GHSGTGKTAL TRAFLKECGK
RQNVRTAHLN AIECYTTKIM LEILLDSLAP DQGDALKVDN MLDFVEQLRR QAATRVEDQG
FLIAVDNAER LRDMDANVLP VLLRLQELTN LNLCVILLSQ LPFEKFYNKT GLSEIVCLHL
AQYNKAETQR ILGSDFQQVR NQLLEQFAQD KKRLEICQEA VTEDFYNNYL NLFLSVFYKA
CRDVPELQLT ARKCLSTYLE PVLDGTVDAT DISRLWRHIA GPLRSALTQI YMRIEKPAEE
VEDFTAIEDQ SVRKLAQSLE LPYYAKFLLI AAFLASHNAA KQDKRLFVKH HGKQRKRMQT
VNARAKTTEK MSTTLGPKSF SIDRLLAIFY AILEEKVGLT CNLLSQISTL VHLNLLSFVS
GEQNIMEGSA RLQCTIGLEF VLQIGKVVGF NVRQYLCDFM
