ORC5_MOUSE
ID ORC5_MOUSE Reviewed; 435 AA.
AC Q9WUV0; Q3TMI6;
DT 21-FEB-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 141.
DE RecName: Full=Origin recognition complex subunit 5;
GN Name=Orc5; Synonyms=Orc5l;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=10460412; DOI=10.1007/s004120050374;
RA Springer J., Kneissl M., Putter V., Grummt F.;
RT "Identification and characterization of MmORC4 and MmORC5, two subunits of
RT the mouse origin of replication recognition complex.";
RL Chromosoma 108:243-249(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Heart, Lung, and Mammary gland;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Component of the origin recognition complex (ORC) that binds
CC origins of replication. DNA-binding is ATP-dependent. The specific DNA
CC sequences that define origins of replication have not been identified
CC yet. ORC is required to assemble the pre-replication complex necessary
CC to initiate DNA replication. {ECO:0000250|UniProtKB:O43913}.
CC -!- SUBUNIT: Component of ORC, a complex composed of at least 6 subunits:
CC ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6. ORC is regulated in a cell-cycle
CC dependent manner. It is sequentially assembled at the exit from
CC anaphase of mitosis and disassembled as cells enter S phase.
CC {ECO:0000250|UniProtKB:O43913}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:O43913}.
CC Chromosome {ECO:0000250|UniProtKB:O43913}.
CC -!- PTM: Multi-mono-ubiquitinated by OBI1; ubiquitination is important for
CC efficient DNA replication origin site activation. Ubiquitination levels
CC are low in mitotic and early G1-phAse cells and are induced in late
CC G1-/early S-phase, peaking in S-phase and decrease toward the end of
CC the cell cycle. {ECO:0000250|UniProtKB:O43913}.
CC -!- SIMILARITY: Belongs to the ORC5 family. {ECO:0000305}.
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DR EMBL; AJ007360; CAB43767.1; -; mRNA.
DR EMBL; AK084683; BAC39250.1; -; mRNA.
DR EMBL; AK144983; BAE26167.1; -; mRNA.
DR EMBL; AK165912; BAE38456.1; -; mRNA.
DR EMBL; AK166537; BAE38839.1; -; mRNA.
DR EMBL; BC006927; AAH06927.1; -; mRNA.
DR CCDS; CCDS19110.1; -.
DR RefSeq; NP_036089.1; NM_011959.2.
DR AlphaFoldDB; Q9WUV0; -.
DR SMR; Q9WUV0; -.
DR BioGRID; 204981; 2.
DR ComplexPortal; CPX-1915; Nuclear origin of replication recognition complex.
DR CORUM; Q9WUV0; -.
DR IntAct; Q9WUV0; 2.
DR MINT; Q9WUV0; -.
DR STRING; 10090.ENSMUSP00000030872; -.
DR iPTMnet; Q9WUV0; -.
DR PhosphoSitePlus; Q9WUV0; -.
DR EPD; Q9WUV0; -.
DR MaxQB; Q9WUV0; -.
DR PaxDb; Q9WUV0; -.
DR PeptideAtlas; Q9WUV0; -.
DR PRIDE; Q9WUV0; -.
DR ProteomicsDB; 293522; -.
DR Antibodypedia; 31233; 97 antibodies from 23 providers.
DR DNASU; 26429; -.
DR Ensembl; ENSMUST00000030872; ENSMUSP00000030872; ENSMUSG00000029012.
DR GeneID; 26429; -.
DR KEGG; mmu:26429; -.
DR UCSC; uc008wpq.1; mouse.
DR CTD; 5001; -.
DR MGI; MGI:1347044; Orc5.
DR VEuPathDB; HostDB:ENSMUSG00000029012; -.
DR eggNOG; KOG2543; Eukaryota.
DR GeneTree; ENSGT00390000009380; -.
DR HOGENOM; CLU_028223_0_0_1; -.
DR InParanoid; Q9WUV0; -.
DR OMA; QLRRWHG; -.
DR OrthoDB; 571556at2759; -.
DR PhylomeDB; Q9WUV0; -.
DR TreeFam; TF101095; -.
DR Reactome; R-MMU-176187; Activation of ATR in response to replication stress.
DR Reactome; R-MMU-68616; Assembly of the ORC complex at the origin of replication.
DR Reactome; R-MMU-68689; CDC6 association with the ORC:origin complex.
DR Reactome; R-MMU-68949; Orc1 removal from chromatin.
DR Reactome; R-MMU-68962; Activation of the pre-replicative complex.
DR BioGRID-ORCS; 26429; 26 hits in 73 CRISPR screens.
DR ChiTaRS; Orc5; mouse.
DR PRO; PR:Q9WUV0; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; Q9WUV0; protein.
DR Bgee; ENSMUSG00000029012; Expressed in primary oocyte and 281 other tissues.
DR ExpressionAtlas; Q9WUV0; baseline and differential.
DR Genevisible; Q9WUV0; MM.
DR GO; GO:0000785; C:chromatin; ISS:UniProtKB.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005664; C:nuclear origin of replication recognition complex; ISS:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0000808; C:origin recognition complex; ISO:MGI.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003688; F:DNA replication origin binding; IBA:GO_Central.
DR GO; GO:0006260; P:DNA replication; TAS:MGI.
DR GO; GO:0006270; P:DNA replication initiation; IBA:GO_Central.
DR GO; GO:0006275; P:regulation of DNA replication; ISS:UniProtKB.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR041664; AAA_16.
DR InterPro; IPR020796; ORC5.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR12705; PTHR12705; 1.
DR Pfam; PF13191; AAA_16; 1.
DR Pfam; PF14630; ORC5_C; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Chromosome; DNA replication; Nucleotide-binding; Nucleus;
KW Reference proteome; Ubl conjugation.
FT CHAIN 1..435
FT /note="Origin recognition complex subunit 5"
FT /id="PRO_0000127093"
FT BINDING 37..44
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
SQ SEQUENCE 435 AA; 50217 MW; 1F334E35379F5C69 CRC64;
MSHLESMVLC REAQVSTLQS LFGERHHFSF PSIFIYGHTA SGKTYVTQTL LKTLELPHAF
VNCVECFTSR LLLEQILNKL SHLNSSDAGC STEMTCETFN DFVQLFKQVT SAEHLKDQTV
YIVLDKAEYL RDMEANLLPG LLRLQELTDR NVTVIFLSEI IWEKFRPNTG CFEPFVLYFP
DYSIGNLQKI LSHDHPPEYS ADFYAAYINI LLGVFYTVCR DLKELRHLAV LNFPKYCEPV
VKGEAGERDT RKLWRNIEPH LKKAMQTVYL REISSSQWEK LQKDNTDPGQ LKGLSAYTHM
ELPYYSKFIL IAAYLASYNP ARTDKRFFLK HHGKIKKTNF LKKHEKTSNH LLGPKPFPLD
RLLAILYSIV DSRVAPTANI FSQITSLVTL QLLTIVGHED QLNGPKYKCT VSLDFIRAIA
RMVNFDIIKY LYDFL
