ORC5_PLAF7
ID ORC5_PLAF7 Reviewed; 899 AA.
AC O96237;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Origin recognition complex subunit 5 {ECO:0000303|PubMed:18554328};
DE Short=PfORC5 {ECO:0000303|PubMed:18554328};
DE EC=3.6.4.- {ECO:0000269|PubMed:18554328};
GN Name=ORC5 {ECO:0000303|PubMed:18554328};
GN ORFNames=PF3D7_0215800 {ECO:0000312|EMBL:CZT98171.1};
OS Plasmodium falciparum (isolate 3D7).
OC Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Haemosporida;
OC Plasmodiidae; Plasmodium; Plasmodium (Laverania).
OX NCBI_TaxID=36329 {ECO:0000312|Proteomes:UP000001450};
RN [1] {ECO:0000312|Proteomes:UP000001450}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=3D7 {ECO:0000312|Proteomes:UP000001450};
RX PubMed=9804551; DOI=10.1126/science.282.5391.1126;
RA Gardner M.J., Tettelin H., Carucci D.J., Cummings L.M., Aravind L.,
RA Koonin E.V., Shallom S.J., Mason T., Yu K., Fujii C., Pederson J., Shen K.,
RA Jing J., Aston C., Lai Z., Schwartz D.C., Pertea M., Salzberg S.L.,
RA Zhou L., Sutton G.G., Clayton R., White O., Smith H.O., Fraser C.M.,
RA Adams M.D., Venter J.C., Hoffman S.L.;
RT "Chromosome 2 sequence of the human malaria parasite Plasmodium
RT falciparum.";
RL Science 282:1126-1132(1998).
RN [2] {ECO:0000312|Proteomes:UP000001450}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=3D7 {ECO:0000312|Proteomes:UP000001450};
RX PubMed=12368864; DOI=10.1038/nature01097;
RA Gardner M.J., Hall N., Fung E., White O., Berriman M., Hyman R.W.,
RA Carlton J.M., Pain A., Nelson K.E., Bowman S., Paulsen I.T., James K.D.,
RA Eisen J.A., Rutherford K.M., Salzberg S.L., Craig A., Kyes S., Chan M.-S.,
RA Nene V., Shallom S.J., Suh B., Peterson J., Angiuoli S., Pertea M.,
RA Allen J., Selengut J., Haft D., Mather M.W., Vaidya A.B., Martin D.M.A.,
RA Fairlamb A.H., Fraunholz M.J., Roos D.S., Ralph S.A., McFadden G.I.,
RA Cummings L.M., Subramanian G.M., Mungall C., Venter J.C., Carucci D.J.,
RA Hoffman S.L., Newbold C., Davis R.W., Fraser C.M., Barrell B.G.;
RT "Genome sequence of the human malaria parasite Plasmodium falciparum.";
RL Nature 419:498-511(2002).
RN [3] {ECO:0000305}
RP CATALYTIC ACTIVITY, INTERACTION WITH PCNA1, SUBCELLULAR LOCATION, AND
RP DEVELOPMENTAL STAGE.
RX PubMed=18554328; DOI=10.1111/j.1365-2958.2008.06316.x;
RA Gupta A., Mehra P., Dhar S.K.;
RT "Plasmodium falciparum origin recognition complex subunit 5: functional
RT characterization and role in DNA replication foci formation.";
RL Mol. Microbiol. 69:646-665(2008).
CC -!- FUNCTION: Component of the origin recognition complex (ORC) that binds
CC origins of replication. {ECO:0000250|UniProtKB:P50874}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216;
CC Evidence={ECO:0000269|PubMed:18554328};
CC -!- SUBUNIT: Component of the origin recognition complex (ORC) (By
CC similarity). Interacts with PCNA1; the interaction occurs during the
CC trophozoite stage but not at the late schizont stage (PubMed:18554328).
CC {ECO:0000250|UniProtKB:P50874, ECO:0000269|PubMed:18554328}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:18554328}.
CC Note=During early-to mid replicating trophozoite stages, colocalizes
CC with PCNA1 and ORC1 to distinct nuclear foci which probably are DNA
CC replication origin sites (PubMed:18554328). Dissociates from PCNA1
CC during the late schizont stage (PubMed:18554328).
CC {ECO:0000269|PubMed:18554328}.
CC -!- DEVELOPMENTAL STAGE: Expressed during the asexual blood stage,
CC specifically during the trophozoite and schizont stages (at protein
CC level). {ECO:0000269|PubMed:18554328}.
CC -!- SIMILARITY: Belongs to the ORC5 family. {ECO:0000305}.
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DR EMBL; LN999943; CZT98171.1; -; Genomic_DNA.
DR RefSeq; XP_001349663.1; XM_001349627.1.
DR AlphaFoldDB; O96237; -.
DR STRING; 5833.PFB0720c; -.
DR PRIDE; O96237; -.
DR EnsemblProtists; CZT98171; CZT98171; PF3D7_0215800.
DR GeneID; 812745; -.
DR KEGG; pfa:PF3D7_0215800; -.
DR VEuPathDB; PlasmoDB:PF3D7_0215800; -.
DR HOGENOM; CLU_341165_0_0_1; -.
DR InParanoid; O96237; -.
DR OMA; DMCKNIL; -.
DR PhylomeDB; O96237; -.
DR Reactome; R-PFA-68616; Assembly of the ORC complex at the origin of replication.
DR Proteomes; UP000001450; Chromosome 2.
DR GO; GO:0005664; C:nuclear origin of replication recognition complex; IBA:GO_Central.
DR GO; GO:0005656; C:nuclear pre-replicative complex; IDA:GeneDB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0005524; F:ATP binding; IDA:GeneDB.
DR GO; GO:0003688; F:DNA replication origin binding; IBA:GO_Central.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006270; P:DNA replication initiation; IBA:GO_Central.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR020796; ORC5.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR12705; PTHR12705; 1.
DR Pfam; PF14630; ORC5_C; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
PE 1: Evidence at protein level;
KW ATP-binding; DNA replication; Hydrolase; Nucleotide-binding; Nucleus;
KW Reference proteome.
FT CHAIN 1..899
FT /note="Origin recognition complex subunit 5"
FT /id="PRO_0000454931"
FT REGION 27..47
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 100..166
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 194..238
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 409..469
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 110..160
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 194..218
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 219..238
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 423..455
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 303..310
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00499"
SQ SEQUENCE 899 AA; 103992 MW; BE50142D7AD64454 CRC64;
MYKIKNNESD INSDDCNETA QECIYGFSSP KKSRKESPIF VQNNDDTCSS NNIYEKKTCS
NTSASSVKKY DKKEQSLCSD INNYNKVNIE GLKINERNYD RINNSEEETN INDDNNDDNN
GDYDDDNNSD DDDDNDDNNN NDDNNNDDDE DVDDFEDIKE NDEYKDPTYS DIYKEAKKCN
IRCENIMNSS VNKKNLEEIN ESDPLNSSDN SMTSSSEESC SEESDKESDK ESDKDGNLYD
EELNEIIEEG YFKELIPAIP HDILQAYISC LKICGFERQV QLHRLINLLG DLRDPISVIQ
ILGLPGMGKT KVVKNFIKLT NVPFAYVNCL MAVYQSGRSA KNVIYHTILK DLSINLLNEF
NEYKKINNIT NYSYDPTKLV PNHVSNTDNF FSILHKLLSF KPEDILNNKR TTENIRSPSN
SNNNKKKKKE QNDSTGKNSK EECNNNEDDD DDNNKNNFNN NNSNNVRFNS NTNYYKDKLY
DRSVVFILDN IRYLVRTHPD LFYALTRIHE YIKGPYNDVT KANKTTRGLC IILINRSPLP
DEIFDGLPQP PTVWFDSYTS EMCKNILYRL YNSMCFESLL TYNDKDLKIY YVKHNKNEFL
IKRNDVILEN DVIYDIWCRY VDYIINVSYK DYKSDFHELL FICSHMWPLF IKPILDGVLE
PIVENMNALQ RNIDTHIRVA TYNHSSHFTF ELIDSVFLNE NNLKNKIDLS FYSKILLVGA
YLASRNLPLT DKRFFNATVK GGAFTLPKKR KGKNKNESIL TLLSKSIPKN FTFIRWLCLT
DCLLVCFFDE QLILNSLICQ QINTLIQLGF ISFSSPNNLS CLVRNSLMNG VQWSGYCGSA
LLNTTTNFSS LTNNIFCETN NSMTYESLDP YTKLVIQVPE ETIRNISKEM KIPLDELII
