ID   ORC5_SCHPO              Reviewed;         455 AA.
AC   O43114; Q9UQW3;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   21-FEB-2001, sequence version 2.
DT   03-AUG-2022, entry version 132.
DE   RecName: Full=Origin recognition complex subunit 5;
GN   Name=orc5; Synonyms=orp5; ORFNames=SPBC646.14c;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=9417919; DOI=10.1006/geno.1997.5003;
RA   Ishiai M., Dean F.B., Okumura K., Abe M., Moon K.-Y., Amin A.A.,
RA   Kagotani K., Taguchi H., Murakami Y., Hanaoka F., O'Donnell M., Hurwitz J.,
RA   Eki T.;
RT   "Isolation of human and fission yeast homologues of the budding yeast
RT   origin recognition complex subunit ORC5: human homologue (ORC5L) maps to
RT   7q22.";
RL   Genomics 46:294-298(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [3]
RP   SUBUNIT.
RX   PubMed=10535928; DOI=10.1073/pnas.96.22.12367;
RA   Moon K.-Y., Kong D., Lee J.-K., Raychaudhuri S., Hurwitz J.;
RT   "Identification and reconstitution of the origin recognition complex from
RT   Schizosaccharomyces pombe.";
RL   Proc. Natl. Acad. Sci. U.S.A. 96:12367-12372(1999).
RN   [4]
RP   CHARACTERIZATION OF ORC, AND INTERACTION WITH CDC18.
RX   PubMed=11850415; DOI=10.1074/jbc.m107710200;
RA   Chuang R.-Y., Chretien L., Dai J., Kelly T.J.;
RT   "Purification and characterization of the Schizosaccharomyces pombe origin
RT   recognition complex: interaction with origin DNA and Cdc18 protein.";
RL   J. Biol. Chem. 277:16920-16927(2002).
CC   -!- FUNCTION: Component of the origin recognition complex (ORC) that binds
CC       origins of replication. It has a role in both chromosomal replication
CC       and mating type transcriptional silencing. ORC binds to multiple sites
CC       within the ars1 origin of DNA replication in an ATP-independent manner.
CC   -!- SUBUNIT: ORC is composed of six subunits. ORC interacts with cdc18,
CC       recruiting it to the ars1 origin of replication.
CC       {ECO:0000269|PubMed:10535928, ECO:0000269|PubMed:11850415}.
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- SIMILARITY: Belongs to the ORC5 family. {ECO:0000305}.
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DR   EMBL; U92539; AAC49897.1; -; mRNA.
DR   EMBL; CU329671; CAA22818.1; -; Genomic_DNA.
DR   PIR; T40590; T40590.
DR   RefSeq; NP_595372.1; NM_001021280.2.
DR   AlphaFoldDB; O43114; -.
DR   SMR; O43114; -.
DR   BioGRID; 277622; 11.
DR   STRING; 4896.SPBC646.14c.1; -.
DR   MaxQB; O43114; -.
DR   PaxDb; O43114; -.
DR   EnsemblFungi; SPBC646.14c.1; SPBC646.14c.1:pep; SPBC646.14c.
DR   GeneID; 2541107; -.
DR   KEGG; spo:SPBC646.14c; -.
DR   PomBase; SPBC646.14c; orc5.
DR   VEuPathDB; FungiDB:SPBC646.14c; -.
DR   eggNOG; KOG2543; Eukaryota.
DR   HOGENOM; CLU_028223_2_0_1; -.
DR   InParanoid; O43114; -.
DR   OMA; QLRRWHG; -.
DR   PhylomeDB; O43114; -.
DR   Reactome; R-SPO-176187; Activation of ATR in response to replication stress.
DR   Reactome; R-SPO-68616; Assembly of the ORC complex at the origin of replication.
DR   Reactome; R-SPO-68689; CDC6 association with the ORC:origin complex.
DR   Reactome; R-SPO-68949; Orc1 removal from chromatin.
DR   Reactome; R-SPO-68962; Activation of the pre-replicative complex.
DR   PRO; PR:O43114; -.
DR   Proteomes; UP000002485; Chromosome II.
DR   GO; GO:0000785; C:chromatin; IDA:PomBase.
DR   GO; GO:0031261; C:DNA replication preinitiation complex; IC:PomBase.
DR   GO; GO:0005664; C:nuclear origin of replication recognition complex; IDA:UniProtKB.
DR   GO; GO:0005656; C:nuclear pre-replicative complex; IC:PomBase.
DR   GO; GO:0043596; C:nuclear replication fork; IC:PomBase.
DR   GO; GO:0005634; C:nucleus; HDA:PomBase.
DR   GO; GO:0005524; F:ATP binding; ISS:UniProtKB.
DR   GO; GO:0016887; F:ATP hydrolysis activity; NAS:PomBase.
DR   GO; GO:0003688; F:DNA replication origin binding; IDA:UniProtKB.
DR   GO; GO:0006270; P:DNA replication initiation; IBA:GO_Central.
DR   GO; GO:1902975; P:mitotic DNA replication initiation; IMP:PomBase.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR020796; ORC5.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR12705; PTHR12705; 1.
DR   Pfam; PF14630; ORC5_C; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; DNA replication; Nucleotide-binding; Nucleus;
KW   Reference proteome.
FT   CHAIN           1..455
FT                   /note="Origin recognition complex subunit 5"
FT                   /id="PRO_0000127095"
FT   BINDING         42..49
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        328
FT                   /note="F -> S (in Ref. 1; AAC49897)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   455 AA;  51762 MW;  B7A6AC2CFF7D2FB5 CRC64;
     MHLYQLEDEL KKNVFCREDQ IKKLSCLLFN KDCRVPSIVL YGVASTAKTF LLRTAFDLSK
     EENVWINLQD CFTVAHFWYR ILIKVGVDKD IALKKGINIS GFIYLLEQAM SKRDYHTFLV
     LDQIDDFAEA STILFSQLAQ LPIVANIPNL SIIFVLHSHP AQYLGTLSIA VIFFPQYTQA
     EILEICQKTP PNLDFLDRSG DSVFEDEIEL SVWMQYCSFL WSVFGVQCLN DYRSFRSVLD
     RYWPKFIQPI VEGDIHPADY AQLHKLAKNF LVSDATVTKR LHIINPTEIK NLLDSKSINL
     SLVSKYLLVS AFLASYNPSR LDAQFFSFSK TSKRRGRKRK QIQDEGVLFS KIPRTAGSKG
     RSAVKISQLT LGPKPFEVER LIAIYYAISS PVEKVLTADV FVQIATLASL KMILSASKGV
     LRSLDSPRYI VNVSREYVLK IADSLSFPLD SYLAG