ORC6_ARATH
ID ORC6_ARATH Reviewed; 284 AA.
AC Q9ZVH3; Q6EWW9;
DT 21-FEB-2001, integrated into UniProtKB/Swiss-Prot.
DT 20-DEC-2005, sequence version 2.
DT 25-MAY-2022, entry version 124.
DE RecName: Full=Origin of replication complex subunit 6 {ECO:0000303|PubMed:16179646};
DE Short=AtORC6 {ECO:0000303|PubMed:16179646};
GN Name=ORC6 {ECO:0000303|PubMed:16179646};
GN OrderedLocusNames=At1g26840 {ECO:0000312|Araport:AT1G26840};
GN ORFNames=T2P11.3 {ECO:0000312|EMBL:AAD14490.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], SUBUNIT, INTERACTION WITH ORC2; ORC3; ORC4 AND
RP ORC5, TISSUE SPECIFICITY, INDUCTION BY E2F AND SUCROSE, GENE FAMILY, AND
RP NOMENCLATURE.
RX PubMed=16179646; DOI=10.1093/nar/gki854;
RA Diaz-Trivino S., Castellano M.M., Sanchez M.P., Ramirez-Parra E.,
RA Desvoyes B., Gutierrez C.;
RT "The genes encoding Arabidopsis ORC subunits are E2F targets and the two
RT ORC1 genes are differently expressed in proliferating and endoreplicating
RT cells.";
RL Nucleic Acids Res. 33:5404-5414(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP SUBUNIT, INTERACTION WITH ORC3, TISSUE SPECIFICITY, INDUCTION BY SUCROSE,
RP AND GENE FAMILY.
RC STRAIN=cv. Columbia;
RX PubMed=15358564; DOI=10.1016/j.febslet.2004.07.088;
RA Masuda H.P., Ramos G.B.A., de Almeida-Engler J., Cabral L.M.,
RA Coqueiro V.M., Macrini C.M.T., Ferreira P.C.G., Hemerly A.S.;
RT "Genome based identification and analysis of the pre-replicative complex of
RT Arabidopsis thaliana.";
RL FEBS Lett. 574:192-202(2004).
RN [5]
RP INDUCTION BY E2F.
RX PubMed=16126853; DOI=10.1104/pp.105.066290;
RA Vandepoele K., Vlieghe K., Florquin K., Hennig L., Beemster G.T.S.,
RA Gruissem W., Van de Peer Y., Inze D., De Veylder L.;
RT "Genome-wide identification of potential plant E2F target genes.";
RL Plant Physiol. 139:316-328(2005).
RN [6]
RP REVIEW ON THE CORE DNA REPLICATION MACHINERY.
RX PubMed=17556508; DOI=10.1104/pp.107.101105;
RA Shultz R.W., Tatineni V.M., Hanley-Bowdoin L., Thompson W.F.;
RT "Genome-wide analysis of the core DNA replication machinery in the higher
RT plants Arabidopsis and rice.";
RL Plant Physiol. 144:1697-1714(2007).
CC -!- FUNCTION: Component of the origin recognition complex (ORC) that binds
CC origins of replication. DNA-binding is ATP-dependent. The specific DNA
CC sequences that define origins of replication have not been identified
CC yet. ORC is required to assemble the pre-replication complex necessary
CC to initiate DNA replication. {ECO:0000250|UniProtKB:Q9Y5N6}.
CC -!- SUBUNIT: Component of the origin recognition complex (ORC) composed of
CC at least ORC1 (ORC1A or ORC1B), ORC2, ORC3, ORC4, ORC5 and ORC6. ORC is
CC regulated in a cell-cycle and development dependent manner. It is
CC sequentially assembled at the exit from anaphase of mitosis and
CC disassembled as cells enter S phase. Interacts directly with ORC2,
CC ORC3, ORC4 and ORC5. {ECO:0000269|PubMed:15358564,
CC ECO:0000269|PubMed:16179646}.
CC -!- INTERACTION:
CC Q9ZVH3; Q38899: ORC2; NbExp=2; IntAct=EBI-2114077, EBI-2114089;
CC Q9ZVH3; Q6EWX1: ORC4; NbExp=2; IntAct=EBI-2114077, EBI-2114176;
CC Q9ZVH3; Q6EWX0: ORC5; NbExp=2; IntAct=EBI-2114077, EBI-2114109;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q9Y5N6}.
CC -!- TISSUE SPECIFICITY: Follow a cell-cycle regulation with a peak at the
CC G1/S-phase (PubMed:16179646). Mostly expressed in siliques, flowers,
CC flower buds and mature leaves, and, to a lower exent, in roots, leaves
CC and stems (PubMed:16179646, PubMed:15358564).
CC {ECO:0000269|PubMed:15358564, ECO:0000269|PubMed:16179646}.
CC -!- INDUCTION: Regulated by E2F (PubMed:16179646, PubMed:16126853).
CC Accumulates rapidly after cell cycle reactivation by sucrose addition
CC following cell cycle arrest mediated by sucrose deprivation
CC (PubMed:16179646, PubMed:15358564). {ECO:0000269|PubMed:15358564,
CC ECO:0000269|PubMed:16126853, ECO:0000269|PubMed:16179646}.
CC -!- SIMILARITY: Belongs to the ORC6 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD14490.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AJ575584; CAE01430.1; -; mRNA.
DR EMBL; AC005508; AAD14490.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE30748.1; -; Genomic_DNA.
DR PIR; B86395; B86395.
DR RefSeq; NP_174006.2; NM_102448.4.
DR AlphaFoldDB; Q9ZVH3; -.
DR SMR; Q9ZVH3; -.
DR IntAct; Q9ZVH3; 5.
DR STRING; 3702.AT1G26840.1; -.
DR PaxDb; Q9ZVH3; -.
DR PRIDE; Q9ZVH3; -.
DR EnsemblPlants; AT1G26840.1; AT1G26840.1; AT1G26840.
DR GeneID; 839227; -.
DR Gramene; AT1G26840.1; AT1G26840.1; AT1G26840.
DR KEGG; ath:AT1G26840; -.
DR Araport; AT1G26840; -.
DR TAIR; locus:2202800; AT1G26840.
DR eggNOG; KOG4557; Eukaryota.
DR HOGENOM; CLU_080569_0_0_1; -.
DR InParanoid; Q9ZVH3; -.
DR OMA; RMFEKLL; -.
DR OrthoDB; 1522235at2759; -.
DR PhylomeDB; Q9ZVH3; -.
DR PRO; PR:Q9ZVH3; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9ZVH3; baseline and differential.
DR Genevisible; Q9ZVH3; AT.
DR GO; GO:0005664; C:nuclear origin of replication recognition complex; IBA:GO_Central.
DR GO; GO:0000808; C:origin recognition complex; ISS:TAIR.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006260; P:DNA replication; ISS:TAIR.
DR GO; GO:0006270; P:DNA replication initiation; IBA:GO_Central.
DR GO; GO:0009555; P:pollen development; IMP:TAIR.
DR InterPro; IPR008721; ORC6.
DR InterPro; IPR020529; ORC6_met/pln.
DR PANTHER; PTHR13394; PTHR13394; 1.
DR Pfam; PF05460; ORC6; 1.
PE 1: Evidence at protein level;
KW DNA replication; DNA-binding; Nucleus; Reference proteome.
FT CHAIN 1..284
FT /note="Origin of replication complex subunit 6"
FT /id="PRO_0000127100"
SQ SEQUENCE 284 AA; 31770 MW; 515ED05705D83C4C CRC64;
MDISDIGRKL SLDNNKLLIR KAAEIRRLCD AQFDSSIIGV GEICKAVICL EIAATRLQII
FDRQAAVKLS GMSEKAYSRS FNSLQNVIGI KIKLNVRELA VQFGCVRVIK SVQNVLSSYK
ERFLASLPAS RRANADFTRP VFTAAAFYLC AKKQKLKVDK LRLIEVCGTS ESEFSCVSTS
MTDLCFDCVG ISKEKKDAKD VKGNRDLLDV LPGKRRLEDG GYSSGDESSC YKRHKKMEEA
KYEDWKSTVV NSIKKNPEKG TKRVIQASLN FPKKSETKEL QVDS
