ORC6_DROME
ID ORC6_DROME Reviewed; 257 AA.
AC Q9Y1B2; Q9V5C5;
DT 21-FEB-2001, integrated into UniProtKB/Swiss-Prot.
DT 21-FEB-2001, sequence version 2.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Origin recognition complex subunit 6;
GN Name=Orc6; ORFNames=CG1584;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=10346817; DOI=10.1101/gad.13.10.1289;
RA Chesnokov I., Gossen M., Remus D., Botchan M.;
RT "Assembly of functionally active Drosophila origin recognition complex from
RT recombinant proteins.";
RL Genes Dev. 13:1289-1296(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: Component of the origin recognition complex (ORC) that binds
CC origins of replication. DNA-binding is ATP-dependent, however specific
CC DNA sequences that define origins of replication have not been
CC identified so far. ORC is required to assemble the pre-replication
CC complex necessary to initiate DNA replication (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: ORC is composed of six subunits.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the ORC6 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF139064; AAD39474.1; -; mRNA.
DR EMBL; AE013599; AAF58890.1; -; Genomic_DNA.
DR EMBL; AY071443; AAL49065.1; -; mRNA.
DR RefSeq; NP_477319.1; NM_057971.6.
DR PDB; 4XGC; X-ray; 3.50 A; F=187-257.
DR PDB; 7JGR; EM; 3.90 A; F=1-257.
DR PDB; 7JGS; EM; 3.20 A; F=1-257.
DR PDB; 7JK2; EM; 3.20 A; F=1-257.
DR PDB; 7JK3; EM; 3.40 A; F=1-257.
DR PDB; 7JK4; EM; 3.40 A; F=1-257.
DR PDB; 7JK5; EM; 3.90 A; F=1-257.
DR PDB; 7JK6; EM; 4.00 A; F=1-257.
DR PDBsum; 4XGC; -.
DR PDBsum; 7JGR; -.
DR PDBsum; 7JGS; -.
DR PDBsum; 7JK2; -.
DR PDBsum; 7JK3; -.
DR PDBsum; 7JK4; -.
DR PDBsum; 7JK5; -.
DR PDBsum; 7JK6; -.
DR AlphaFoldDB; Q9Y1B2; -.
DR SMR; Q9Y1B2; -.
DR BioGRID; 61846; 20.
DR DIP; DIP-49258N; -.
DR IntAct; Q9Y1B2; 11.
DR STRING; 7227.FBpp0087566; -.
DR PaxDb; Q9Y1B2; -.
DR PRIDE; Q9Y1B2; -.
DR DNASU; 36017; -.
DR EnsemblMetazoa; FBtr0088482; FBpp0087566; FBgn0023180.
DR GeneID; 36017; -.
DR KEGG; dme:Dmel_CG1584; -.
DR CTD; 23594; -.
DR FlyBase; FBgn0023180; Orc6.
DR VEuPathDB; VectorBase:FBgn0023180; -.
DR eggNOG; KOG4557; Eukaryota.
DR GeneTree; ENSGT00390000007370; -.
DR HOGENOM; CLU_067825_0_0_1; -.
DR InParanoid; Q9Y1B2; -.
DR OMA; RMFEKLL; -.
DR OrthoDB; 1522235at2759; -.
DR PhylomeDB; Q9Y1B2; -.
DR Reactome; R-DME-176187; Activation of ATR in response to replication stress.
DR Reactome; R-DME-68616; Assembly of the ORC complex at the origin of replication.
DR Reactome; R-DME-68689; CDC6 association with the ORC:origin complex.
DR Reactome; R-DME-68949; Orc1 removal from chromatin.
DR Reactome; R-DME-68962; Activation of the pre-replicative complex.
DR SignaLink; Q9Y1B2; -.
DR BioGRID-ORCS; 36017; 1 hit in 1 CRISPR screen.
DR GenomeRNAi; 36017; -.
DR PRO; PR:Q9Y1B2; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0023180; Expressed in secondary oocyte and 28 other tissues.
DR Genevisible; Q9Y1B2; DM.
DR GO; GO:0005664; C:nuclear origin of replication recognition complex; IDA:FlyBase.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0000808; C:origin recognition complex; IDA:FlyBase.
DR GO; GO:0005886; C:plasma membrane; IDA:FlyBase.
DR GO; GO:0032156; C:septin cytoskeleton; IDA:FlyBase.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0005096; F:GTPase activator activity; IDA:FlyBase.
DR GO; GO:0042803; F:protein homodimerization activity; IDA:FlyBase.
DR GO; GO:0006260; P:DNA replication; IMP:FlyBase.
DR GO; GO:0006270; P:DNA replication initiation; IDA:FlyBase.
DR GO; GO:0000278; P:mitotic cell cycle; IMP:FlyBase.
DR GO; GO:0043547; P:positive regulation of GTPase activity; IDA:FlyBase.
DR GO; GO:0032185; P:septin cytoskeleton organization; IDA:FlyBase.
DR InterPro; IPR008721; ORC6.
DR InterPro; IPR020529; ORC6_met/pln.
DR PANTHER; PTHR13394; PTHR13394; 1.
DR Pfam; PF05460; ORC6; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA replication; DNA-binding; Nucleus; Reference proteome.
FT CHAIN 1..257
FT /note="Origin recognition complex subunit 6"
FT /id="PRO_0000127099"
FT REGION 186..222
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 73
FT /note="G -> A (in Ref. 1; AAD39474)"
FT /evidence="ECO:0000305"
FT CONFLICT 213
FT /note="E -> D (in Ref. 1; AAD39474)"
FT /evidence="ECO:0000305"
FT HELIX 225..237
FT /evidence="ECO:0007829|PDB:7JGS"
SQ SEQUENCE 257 AA; 29239 MW; 19537834E47AFDDF CRC64;
MTTLIEQLIT KMGLREEPNV LEKTTELVRL LELRSTNVPL QINEYGKIVL CADLASCMIG
IAFDKEQALK LSGLRKSQYL NNKRMFEKLL DLNKLASVND ICVQLGLNEV ARKAEELMTL
FKGVAATEDM GTDTSHPQYA TMAVFQACRL LKKKVSKSKL MPFSNLRPSQ FQLLEQQWER
MIAKHHKESK VPSSTDMEGK LKENQNENIK GHEAKKAHKP PPEDYEIWKA RMLAKAQAKL
KELEASQSHM DSQLLEA
