ORCO_AEDAE
ID ORCO_AEDAE Reviewed; 478 AA.
AC Q178U6; Q6PSG4;
DT 19-MAR-2014, integrated into UniProtKB/Swiss-Prot.
DT 25-JUL-2006, sequence version 1.
DT 25-MAY-2022, entry version 91.
DE RecName: Full=Odorant receptor coreceptor;
DE AltName: Full=Gustatory and odorant receptor 7;
GN Name=SGPRor7; Synonyms=Orco; ORFNames=AAEL005776;
OS Aedes aegypti (Yellowfever mosquito) (Culex aegypti).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Nematocera; Culicoidea; Culicidae;
OC Culicinae; Aedini; Aedes; Stegomyia.
OX NCBI_TaxID=7159;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LVPib12;
RX PubMed=17510324; DOI=10.1126/science.1138878;
RA Nene V., Wortman J.R., Lawson D., Haas B.J., Kodira C.D., Tu Z.J.,
RA Loftus B.J., Xi Z., Megy K., Grabherr M., Ren Q., Zdobnov E.M., Lobo N.F.,
RA Campbell K.S., Brown S.E., Bonaldo M.F., Zhu J., Sinkins S.P.,
RA Hogenkamp D.G., Amedeo P., Arensburger P., Atkinson P.W., Bidwell S.L.,
RA Biedler J., Birney E., Bruggner R.V., Costas J., Coy M.R., Crabtree J.,
RA Crawford M., DeBruyn B., DeCaprio D., Eiglmeier K., Eisenstadt E.,
RA El-Dorry H., Gelbart W.M., Gomes S.L., Hammond M., Hannick L.I.,
RA Hogan J.R., Holmes M.H., Jaffe D., Johnston S.J., Kennedy R.C., Koo H.,
RA Kravitz S., Kriventseva E.V., Kulp D., Labutti K., Lee E., Li S.,
RA Lovin D.D., Mao C., Mauceli E., Menck C.F., Miller J.R., Montgomery P.,
RA Mori A., Nascimento A.L., Naveira H.F., Nusbaum C., O'Leary S.B., Orvis J.,
RA Pertea M., Quesneville H., Reidenbach K.R., Rogers Y.-H.C., Roth C.W.,
RA Schneider J.R., Schatz M., Shumway M., Stanke M., Stinson E.O.,
RA Tubio J.M.C., Vanzee J.P., Verjovski-Almeida S., Werner D., White O.R.,
RA Wyder S., Zeng Q., Zhao Q., Zhao Y., Hill C.A., Raikhel A.S., Soares M.B.,
RA Knudson D.L., Lee N.H., Galagan J., Salzberg S.L., Paulsen I.T.,
RA Dimopoulos G., Collins F.H., Bruce B., Fraser-Liggett C.M., Severson D.W.;
RT "Genome sequence of Aedes aegypti, a major arbovirus vector.";
RL Science 316:1718-1723(2007).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 10-478, FUNCTION, AND TISSUE SPECIFICITY.
RC STRAIN=Costa Rica;
RX PubMed=15201207; DOI=10.1093/chemse/bjh041;
RA Melo A.C., Rutzler M., Pitts R.J., Zwiebel L.J.;
RT "Identification of a chemosensory receptor from the yellow fever mosquito,
RT Aedes aegypti, that is highly conserved and expressed in olfactory and
RT gustatory organs.";
RL Chem. Senses 29:403-410(2004).
RN [3]
RP FUNCTION.
RX PubMed=21738794; DOI=10.1371/journal.pone.0021785;
RA Grant A.J., Dickens J.C.;
RT "Functional characterization of the octenol receptor neuron on the
RT maxillary palps of the yellow fever mosquito, Aedes aegypti.";
RL PLoS ONE 6:E21785-E21785(2011).
RN [4]
RP FUNCTION, AND INTERACTION WITH ODORANT RECEPTORS.
RX PubMed=22269900; DOI=10.1016/j.neuropharm.2012.01.004;
RA Bohbot J.D., Dickens J.C.;
RT "Odorant receptor modulation: ternary paradigm for mode of action of insect
RT repellents.";
RL Neuropharmacology 62:2086-2095(2012).
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=23719379; DOI=10.1038/nature12206;
RA DeGennaro M., McBride C.S., Seeholzer L., Nakagawa T., Dennis E.J.,
RA Goldman C., Jasinskiene N., James A.A., Vosshall L.B.;
RT "orco mutant mosquitoes lose strong preference for humans and are not
RT repelled by volatile DEET.";
RL Nature 498:487-491(2013).
CC -!- FUNCTION: Odorant coreceptor which complexes with conventional odorant
CC receptors (ORs) to form odorant-sensing units, providing sensitive and
CC prolonged odorant signaling and calcium permeability. Orco is a
CC universal and integral part of the functional odorant receptor,
CC involved in the dendritic localization of other olfactory receptors.
CC Plays a key role in preferred attraction of females for humans over
CC non-human hosts for blood feeding. Human attraction plays a crucial
CC role in the transmission of dengue and yellow fever by the mosquito.
CC Also required for the response to the insect repellent IR3535; or to
CC N,N-Diethyl-meta-toluamide (DEET), the most widely used insect
CC repellent worldwide. {ECO:0000269|PubMed:15201207,
CC ECO:0000269|PubMed:21738794, ECO:0000269|PubMed:22269900,
CC ECO:0000269|PubMed:23719379}.
CC -!- SUBUNIT: Heterodimer with conventional odorant receptors (ORs).
CC Complexes exist early in the endomembrane system in olfactory sensory
CC neurons (OSNs), coupling these complexes to the conserved ciliary
CC trafficking pathway.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein.
CC -!- TISSUE SPECIFICITY: Found specifically within most antennal and
CC maxillary palp sensilla, as well as in a subset of proboscis sensilla.
CC {ECO:0000269|PubMed:15201207}.
CC -!- DEVELOPMENTAL STAGE: Expressed in adult chemosensory tissues and during
CC several stages of development.
CC -!- DISRUPTION PHENOTYPE: Impairs both honey and host odor attraction.
CC Leads to DEET insensitivity. {ECO:0000269|PubMed:23719379}.
CC -!- MISCELLANEOUS: The atypical heteromeric and topological design of the
CC odorant receptors appears to be an insect-specific solution for odor
CC recognition, making the OR/Orco complex an attractive target for the
CC development of highly selective insect repellents to disrupt olfactory-
CC mediated host-seeking behaviors of insect disease vectors. Odor-evoked
CC OR currents are independent of known G-protein-coupled second messenger
CC pathways. The homomeric Orco channel is thought to be a cyclic-
CC nucleotide-gated ion channel that depolarizes the olfactory receptor
CC neuron. While many natural and synthetic odorants have been shown to
CC agonize Orco/Or complexes, only a single direct Orco agonist, VUAA1,
CC has been described. On the contrary, amiloride derivatives block
CC Orco/Or complexes.
CC -!- SIMILARITY: Belongs to the insect chemoreceptor superfamily.
CC Heteromeric odorant receptor channel (TC 1.A.69) family. Orco
CC subfamily. {ECO:0000305}.
CC -!- WEB RESOURCE: Name=Protein Spotlight; Note=The senses confused - Issue
CC 161 of June 2014;
CC URL="https://web.expasy.org/spotlight/back_issues/161/";
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DR EMBL; CH477359; EAT42706.1; -; Genomic_DNA.
DR EMBL; AY582943; AAT01220.1; -; mRNA.
DR RefSeq; XP_001651426.1; XM_001651376.1.
DR AlphaFoldDB; Q178U6; -.
DR SMR; Q178U6; -.
DR STRING; 7159.AAEL005776-PA; -.
DR VEuPathDB; VectorBase:AAEL005776; -.
DR eggNOG; ENOG502QR02; Eukaryota.
DR HOGENOM; CLU_045605_0_0_1; -.
DR InParanoid; Q178U6; -.
DR OMA; QIYYVLF; -.
DR OrthoDB; 588157at2759; -.
DR PhylomeDB; Q178U6; -.
DR Proteomes; UP000008820; Chromosome 3.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005549; F:odorant binding; IEA:InterPro.
DR GO; GO:0004984; F:olfactory receptor activity; IMP:UniProtKB.
DR GO; GO:0042755; P:eating behavior; IMP:UniProtKB.
DR GO; GO:0007165; P:signal transduction; IEA:UniProtKB-KW.
DR InterPro; IPR004117; 7tm6_olfct_rcpt.
DR PANTHER; PTHR21137; PTHR21137; 1.
DR Pfam; PF02949; 7tm_6; 1.
PE 1: Evidence at protein level;
KW Behavior; Cell membrane; Glycoprotein; Membrane; Olfaction; Receptor;
KW Reference proteome; Sensory transduction; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..478
FT /note="Odorant receptor coreceptor"
FT /id="PRO_0000425597"
FT TOPO_DOM 1..43
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 44..64
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 65..73
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 74..94
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 95..133
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 134..154
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 155..187
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 188..208
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 209..343
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 344..364
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 365..382
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 383..403
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 404..454
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 455..475
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 476..478
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT CARBOHYD 167
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 13
FT /note="L -> A (in Ref. 2; AAT01220)"
FT /evidence="ECO:0000305"
FT CONFLICT 195
FT /note="Missing (in Ref. 2; AAT01220)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 478 AA; 54277 MW; C897FBA5C549D635 CRC64;
MNVQPTKYHG LVLDLMPNIR LMQGFGHFLF RYVNGPVLIR KLYSWWNLIM ILLQYFAIMG
NLVMNTGDVN ELTANTITTL FFTHSVTKFI YVAVNSEHFY RTLGIWNQPN SHSLFAESDA
RYHSIALAKM RKLLVMVMVT TVLSVVAWIT ITFFGDSVKN VFDKETNETY TVEIPRLPIK
AWYPWDAMSG VPYFFSFIYQ AYFLLFSMCQ ANLADVMFCS WLLFTCEQLQ HLKGIMRPLM
ELSATLDTYR PNSAALFRVA SAGSKSELIL NEEKDPDTKD FDLNGIYNSK ADWGAQFRAP
STLQTFGDNG INGNPNGLTK KQELMVRSAI KYWVERHKHV VRLVSAIGET YGAALLLHML
TSTIKLTLLA YQATKIDALN VYGLTVIGYL VYALAQVFLF CIFGNRLIEE SSSVMEAAYS
CHWYDGSEEA KTFVQIVCQQ CQKAMTISGA KFFTVSLDLF ASVLGAVVTY FMVLVQLK
