ORCO_ANOGA
ID ORCO_ANOGA Reviewed; 478 AA.
AC Q7QCC7; Q6UR07; Q6UR08;
DT 24-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 3.
DT 25-MAY-2022, entry version 98.
DE RecName: Full=Odorant receptor coreceptor;
DE AltName: Full=AgOr7;
DE AltName: Full=Gustatory and odorant receptor 7;
GN Name=Orco; Synonyms=GPRor7; ORFNames=AGAP002560;
OS Anopheles gambiae (African malaria mosquito).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Nematocera; Culicoidea; Culicidae;
OC Anophelinae; Anopheles.
OX NCBI_TaxID=7165;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAR14938.1}
RP NUCLEOTIDE SEQUENCE [MRNA], NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 148-478,
RP FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=G3 {ECO:0000312|EMBL:AAR14938.1};
RX PubMed=15037749; DOI=10.1073/pnas.0308146101;
RA Pitts R.J., Fox A.N., Zwiebel L.J.;
RT "A highly conserved candidate chemoreceptor expressed in both olfactory and
RT gustatory tissues in the malaria vector Anopheles gambiae.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:5058-5063(2004).
RN [2] {ECO:0000312|EMBL:AAX14774.1}
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=15723778; DOI=10.1016/j.cub.2005.02.007;
RA Jones W.D., Nguyen T.-A.T., Kloss B., Lee K.J., Vosshall L.B.;
RT "Functional conservation of an insect odorant receptor gene across 250
RT million years of evolution.";
RL Curr. Biol. 15:R119-R121(2005).
RN [3] {ECO:0000312|EMBL:EAA08038.3}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PEST {ECO:0000312|EMBL:EAA08038.3};
RX PubMed=12364791; DOI=10.1126/science.1076181;
RA Holt R.A., Subramanian G.M., Halpern A., Sutton G.G., Charlab R.,
RA Nusskern D.R., Wincker P., Clark A.G., Ribeiro J.M.C., Wides R.,
RA Salzberg S.L., Loftus B.J., Yandell M.D., Majoros W.H., Rusch D.B., Lai Z.,
RA Kraft C.L., Abril J.F., Anthouard V., Arensburger P., Atkinson P.W.,
RA Baden H., de Berardinis V., Baldwin D., Benes V., Biedler J., Blass C.,
RA Bolanos R., Boscus D., Barnstead M., Cai S., Center A., Chaturverdi K.,
RA Christophides G.K., Chrystal M.A.M., Clamp M., Cravchik A., Curwen V.,
RA Dana A., Delcher A., Dew I., Evans C.A., Flanigan M.,
RA Grundschober-Freimoser A., Friedli L., Gu Z., Guan P., Guigo R.,
RA Hillenmeyer M.E., Hladun S.L., Hogan J.R., Hong Y.S., Hoover J.,
RA Jaillon O., Ke Z., Kodira C.D., Kokoza E., Koutsos A., Letunic I.,
RA Levitsky A.A., Liang Y., Lin J.-J., Lobo N.F., Lopez J.R., Malek J.A.,
RA McIntosh T.C., Meister S., Miller J.R., Mobarry C., Mongin E., Murphy S.D.,
RA O'Brochta D.A., Pfannkoch C., Qi R., Regier M.A., Remington K., Shao H.,
RA Sharakhova M.V., Sitter C.D., Shetty J., Smith T.J., Strong R., Sun J.,
RA Thomasova D., Ton L.Q., Topalis P., Tu Z.J., Unger M.F., Walenz B.,
RA Wang A.H., Wang J., Wang M., Wang X., Woodford K.J., Wortman J.R., Wu M.,
RA Yao A., Zdobnov E.M., Zhang H., Zhao Q., Zhao S., Zhu S.C., Zhimulev I.,
RA Coluzzi M., della Torre A., Roth C.W., Louis C., Kalush F., Mural R.J.,
RA Myers E.W., Adams M.D., Smith H.O., Broder S., Gardner M.J., Fraser C.M.,
RA Birney E., Bork P., Brey P.T., Venter J.C., Weissenbach J., Kafatos F.C.,
RA Collins F.H., Hoffman S.L.;
RT "The genome sequence of the malaria mosquito Anopheles gambiae.";
RL Science 298:129-149(2002).
RN [4]
RP FUNCTION, AND INTERACTION WITH ODORANT RECEPTORS.
RX PubMed=22174894; DOI=10.1371/journal.pone.0028774;
RA Pask G.M., Jones P.L., Rutzler M., Rinker D.C., Zwiebel L.J.;
RT "Heteromeric Anopheline odorant receptors exhibit distinct channel
RT properties.";
RL PLoS ONE 6:E28774-E28774(2011).
RN [5]
RP FUNCTION, INTERACTION WITH ODORANT RECEPTORS, AND AGONIST VUAA1.
RX PubMed=21555561; DOI=10.1073/pnas.1102425108;
RA Jones P.L., Pask G.M., Rinker D.C., Zwiebel L.J.;
RT "Functional agonism of insect odorant receptor ion channels.";
RL Proc. Natl. Acad. Sci. U.S.A. 108:8821-8825(2011).
RN [6]
RP FUNCTION, AND AGONIST VUAA1.
RX PubMed=22272331; DOI=10.1371/journal.pone.0030304;
RA Jones P.L., Pask G.M., Romaine I.M., Taylor R.W., Reid P.R., Waterson A.G.,
RA Sulikowski G.A., Zwiebel L.J.;
RT "Allosteric antagonism of insect odorant receptor ion channels.";
RL PLoS ONE 7:E30304-E30304(2012).
RN [7]
RP FUNCTION, INTERACTION WITH ODORANT RECEPTORS, AND BLOCKADE BY AMILORIDE
RP DERIVATIVES.
RX PubMed=23292750; DOI=10.1093/chemse/bjs100;
RA Pask G.M., Bobkov Y.V., Corey E.A., Ache B.W., Zwiebel L.J.;
RT "Blockade of insect odorant receptor currents by amiloride derivatives.";
RL Chem. Senses 38:221-229(2013).
CC -!- FUNCTION: Odorant coreceptor which complexes with conventional odorant
CC receptors (ORs) to form odorant-sensing units, providing sensitive and
CC prolonged odorant signaling and calcium permeability. Orco is a
CC universal and integral part of the functional odorant receptor,
CC involved in the dendritic localization of other olfactory receptors.
CC Can form functional ion channels in the absence of an odor-binding OR.
CC Plays a key role in preferred attraction of females for humans over
CC non-human hosts for blood feeding. Human attraction plays a crucial
CC role in the transmission of Plasmodium protozoans by the mosquito
CC leading to infection diseases like malaria. Also required for the
CC response to N,N-Diethyl-meta-toluamide (DEET), the most widely used
CC insect repellent worldwide. {ECO:0000269|PubMed:15037749,
CC ECO:0000269|PubMed:21555561, ECO:0000269|PubMed:22174894,
CC ECO:0000269|PubMed:22272331, ECO:0000269|PubMed:23292750}.
CC -!- SUBUNIT: Heterodimer with conventional odorant receptors (ORs).
CC Complexes exist early in the endomembrane system in olfactory sensory
CC neurons (OSNs), coupling these complexes to the conserved ciliary
CC trafficking pathway.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in olfactory and gustatory organs of both
CC adult and immature stages. Highest expression is seen in adult antennae
CC and the maxillary palps. Lower expression also seen in proboscis and
CC legs. Within the antenna, expression originates in cell bodies and
CC projects into the lumen of an individual sensillum, presumably along
CC the dendritic extension of the neuron. Within the maxillary palps,
CC expression is seen in a small number of cell bodies and in projections
CC into the sensillar cone. Within the probiscus, expression is seen in a
CC single type of sensillum on the outer surface of the labellar lobes.
CC {ECO:0000269|PubMed:15037749}.
CC -!- DEVELOPMENTAL STAGE: Expressed in preimago stages, including early
CC stage larvae, late-stage larvae, and pupae, but not in embryos.
CC {ECO:0000269|PubMed:15037749}.
CC -!- MISCELLANEOUS: The atypical heteromeric and topological design of the
CC odorant receptors appears to be an insect-specific solution for odor
CC recognition, making the OR/Orco complex an attractive target for the
CC development of highly selective insect repellents to disrupt olfactory-
CC mediated host-seeking behaviors of insect disease vectors. Odor-evoked
CC OR currents are independent of known G-protein-coupled second messenger
CC pathways. The homomeric Orco channel is thought to be a cyclic-
CC nucleotide-gated ion channel that depolarizes the olfactory receptor
CC neuron. While many natural and synthetic odorants have been shown to
CC agonize Orco/Or complexes, only a single direct Orco agonist, VUAA1,
CC has been described. On the contrary, amiloride derivatives block
CC Orco/Or complexes.
CC -!- SIMILARITY: Belongs to the insect chemoreceptor superfamily.
CC Heteromeric odorant receptor channel (TC 1.A.69) family. Orco
CC subfamily. {ECO:0000305}.
CC -!- WEB RESOURCE: Name=Protein Spotlight; Note=The senses confused - Issue
CC 161 of June 2014;
CC URL="https://web.expasy.org/spotlight/back_issues/161/";
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DR EMBL; AY363725; AAR14938.1; -; mRNA.
DR EMBL; AY363726; AAR14939.1; -; Genomic_DNA.
DR EMBL; AY843205; AAX14774.1; -; mRNA.
DR EMBL; AAAB01008859; EAA08038.3; -; Genomic_DNA.
DR RefSeq; XP_312379.3; XM_312379.3.
DR AlphaFoldDB; Q7QCC7; -.
DR SMR; Q7QCC7; -.
DR STRING; 7165.AGAP002560-PA; -.
DR PaxDb; Q7QCC7; -.
DR GeneID; 1273405; -.
DR KEGG; aga:AgaP_AGAP002560; -.
DR CTD; 1273405; -.
DR VEuPathDB; VectorBase:AGAP002560; -.
DR eggNOG; ENOG502QR02; Eukaryota.
DR HOGENOM; CLU_045605_0_0_1; -.
DR InParanoid; Q7QCC7; -.
DR OMA; QIYYVLF; -.
DR OrthoDB; 588157at2759; -.
DR PhylomeDB; Q7QCC7; -.
DR Proteomes; UP000007062; Chromosome 2R.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005549; F:odorant binding; IEA:InterPro.
DR GO; GO:0004984; F:olfactory receptor activity; IMP:UniProtKB.
DR GO; GO:0008527; F:taste receptor activity; IMP:UniProtKB.
DR GO; GO:0007610; P:behavior; IEA:UniProtKB-KW.
DR GO; GO:0050911; P:detection of chemical stimulus involved in sensory perception of smell; IBA:GO_Central.
DR GO; GO:0007608; P:sensory perception of smell; IMP:UniProtKB.
DR GO; GO:0050909; P:sensory perception of taste; IMP:UniProtKB.
DR GO; GO:0007165; P:signal transduction; IEA:UniProtKB-KW.
DR InterPro; IPR004117; 7tm6_olfct_rcpt.
DR PANTHER; PTHR21137; PTHR21137; 1.
DR Pfam; PF02949; 7tm_6; 1.
PE 1: Evidence at protein level;
KW Behavior; Cell membrane; Glycoprotein; Membrane; Olfaction; Receptor;
KW Reference proteome; Sensory transduction; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..478
FT /note="Odorant receptor coreceptor"
FT /id="PRO_0000296373"
FT TOPO_DOM 1..41
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 42..62
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 63..73
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 74..94
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 95..133
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 134..154
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 155..188
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 189..209
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 210..338
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 339..361
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 362..382
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 383..403
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 404..454
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 455..475
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 476..478
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT CARBOHYD 167
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 478 AA; 54020 MW; 88514EB621C9F8C3 CRC64;
MQVQPTKYVG LVADLMPNIR LMQASGHFLF RYVTGPILIR KVYSWWTLAM VLIQFFAILG
NLATNADDVN ELTANTITTL FFTHSVTKFI YFAVNSENFY RTLAIWNQTN THPLFAESDA
RYHSIALAKM RKLLVLVMAT TVLSVVAWVT ITFFGESVKT VLDKATNETY TVDIPRLPIK
SWYPWNAMSG PAYIFSFIYQ IYFLLFSMVQ SNLADVMFCS WLLLACEQLQ HLKGIMRSLM
ELSASLDTYR PNSSQLFRAI SAGSKSELII NEEKDPDVKD FDLSGIYSSK ADWGAQFRAP
STLQTFDENG RNGNPNGLTR KQEMMVRSAI KYWVERHKHV VRLVSAIGDT YGPALLLHML
TSTIKLTLLA YQATKIDGVN VYGLTVIGYL CYALAQVFLF CIFGNRLIEE SSSVMEAAYS
CHWYDGSEEA KTFVQIVCQQ CQKAMTISGA KFFTVSLDLF ASVLGAVVTY FMVLVQLK
