ORCO_HARSA
ID ORCO_HARSA Reviewed; 479 AA.
AC E2BJ30;
DT 25-OCT-2017, integrated into UniProtKB/Swiss-Prot.
DT 30-NOV-2010, sequence version 1.
DT 25-MAY-2022, entry version 39.
DE RecName: Full=Odorant receptor coreceptor {ECO:0000303|PubMed:28802043};
GN Name=Orco {ECO:0000303|PubMed:28802043};
GN ORFNames=EAI_09968 {ECO:0000312|EMBL:EFN84180.1};
OS Harpegnathos saltator (Jerdon's jumping ant).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Formicoidea;
OC Formicidae; Ponerinae; Ponerini; Harpegnathos.
OX NCBI_TaxID=610380;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=R22 G/1;
RX PubMed=20798317; DOI=10.1126/science.1192428;
RA Bonasio R., Zhang G., Ye C., Mutti N.S., Fang X., Qin N., Donahue G.,
RA Yang P., Li Q., Li C., Zhang P., Huang Z., Berger S.L., Reinberg D.,
RA Wang J., Liebig J.;
RT "Genomic comparison of the ants Camponotus floridanus and Harpegnathos
RT saltator.";
RL Science 329:1068-1071(2010).
RN [2]
RP FUNCTION, DISRUPTION PHENOTYPE, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=28802043; DOI=10.1016/j.cell.2017.06.051;
RA Yan H., Opachaloemphan C., Mancini G., Yang H., Gallitto M., Mlejnek J.,
RA Leibholz A., Haight K., Ghaninia M., Huo L., Perry M., Slone J., Zhou X.,
RA Traficante M., Penick C.A., Dolezal K., Gokhale K., Stevens K.,
RA Fetter-Pruneda I., Bonasio R., Zwiebel L.J., Berger S.L., Liebig J.,
RA Reinberg D., Desplan C.;
RT "An engineered orco mutation produces aberrant social behavior and
RT defective neural development in ants.";
RL Cell 170:736-747(2017).
CC -!- FUNCTION: Odorant coreceptor which complexes with conventional odorant
CC receptors (ORs) to form odorant-sensing units, providing sensitive and
CC prolonged odorant signaling and calcium permeability (By similarity).
CC Obligate coreceptor of all odorant receptors (By similarity). Orco is a
CC universal and integral part of the functional odorant receptor,
CC involved in the dendritic localization of other olfactory receptors.
CC Can form functional ion channels in the absence of an odor-binding
CC odorant receptor (By similarity). Plays a central role in the
CC perception of olfactory stimuli in ants and is essential for ant social
CC organization (PubMed:28802043). Required for pheromone sensing and
CC mating behavior (PubMed:28802043). Also required for the development
CC and maintenance of odorant receptor neurons (ORNs) and of antennal lobe
CC glomeruli (PubMed:28802043). {ECO:0000250|UniProtKB:Q7QCC7,
CC ECO:0000269|PubMed:28802043}.
CC -!- SUBUNIT: Heterodimer with conventional odorant receptors (ORs).
CC {ECO:0000250|UniProtKB:Q7QCC7}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- DISRUPTION PHENOTYPE: Impaired behavior and decreased reproductive
CC performance, probably due to loss of pheromone sensing. Ants show
CC strongly decreased sensitivity to various pure semiochemicals delivered
CC in puffs of air, and display increased levels of wandering outside of
CC the nest. In a solitary environment, homozygous mutants also display an
CC abnormal antennal twitching behavior: they quickly move their antennae,
CC similar to the quick antennal movement observed during dueling among
CC workers in the transition to gamergates. Mating and reproduction are
CC impaired: worker dueling and the transition to gamergates in the
CC absence of a reproductive queen or gamergates. Ants also display gross
CC neuroanatomical defects in the antennal lobe, with a dramatic decrease
CC in the number of odorant receptor neurons and antennal lobe glomeruli.
CC {ECO:0000269|PubMed:28802043}.
CC -!- MISCELLANEOUS: In contrast to most eusocial ant species, where only the
CC queen can transmit genetic information, workers in H.saltator ants can
CC be converted into gamergates (pseudoqueens) that can found entire
CC colonies, facilitating genetic studies (PubMed:28802043).
CC {ECO:0000269|PubMed:28802043}.
CC -!- SIMILARITY: Belongs to the insect chemoreceptor superfamily.
CC Heteromeric odorant receptor channel (TC 1.A.69) family. Orco
CC subfamily. {ECO:0000305}.
CC -!- WEB RESOURCE: Name=Protein Spotlight; Note=Whispers - Issue 197 of
CC November 2017;
CC URL="https://web.expasy.org/spotlight/back_issues/197/";
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DR EMBL; GL448558; EFN84180.1; -; Genomic_DNA.
DR RefSeq; XP_011139767.1; XM_011141465.2.
DR AlphaFoldDB; E2BJ30; -.
DR SMR; E2BJ30; -.
DR STRING; 610380.E2BJ30; -.
DR GeneID; 105183395; -.
DR KEGG; hst:105183395; -.
DR InParanoid; E2BJ30; -.
DR OMA; QIYYVLF; -.
DR OrthoDB; 588157at2759; -.
DR PhylomeDB; E2BJ30; -.
DR Proteomes; UP000008237; Unassembled WGS sequence.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005549; F:odorant binding; IEA:InterPro.
DR GO; GO:0004984; F:olfactory receptor activity; IMP:UniProtKB.
DR GO; GO:0007469; P:antennal development; IMP:UniProtKB.
DR GO; GO:0050911; P:detection of chemical stimulus involved in sensory perception of smell; IMP:UniProtKB.
DR GO; GO:0043695; P:detection of pheromone; IMP:UniProtKB.
DR GO; GO:0007617; P:mating behavior; IMP:UniProtKB.
DR GO; GO:0042048; P:olfactory behavior; IMP:UniProtKB.
DR GO; GO:0019236; P:response to pheromone; IMP:UniProtKB.
DR GO; GO:0007165; P:signal transduction; IEA:UniProtKB-KW.
DR GO; GO:0035176; P:social behavior; IMP:UniProtKB.
DR InterPro; IPR004117; 7tm6_olfct_rcpt.
DR PANTHER; PTHR21137; PTHR21137; 1.
DR Pfam; PF02949; 7tm_6; 1.
PE 1: Evidence at protein level;
KW Behavior; Cell membrane; Glycoprotein; Membrane; Olfaction; Receptor;
KW Reference proteome; Sensory transduction; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..479
FT /note="Odorant receptor coreceptor"
FT /id="PRO_0000442002"
FT TOPO_DOM 1..43
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:28802043"
FT TRANSMEM 44..64
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 65..73
FT /note="Extracellular"
FT /evidence="ECO:0000305|PubMed:28802043"
FT TRANSMEM 74..94
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 95..133
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:28802043"
FT TRANSMEM 134..154
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 155..190
FT /note="Extracellular"
FT /evidence="ECO:0000305, ECO:0000305|PubMed:28802043"
FT TRANSMEM 191..211
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 212..350
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:28802043"
FT TRANSMEM 351..371
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 372..383
FT /note="Extracellular"
FT /evidence="ECO:0000305, ECO:0000305|PubMed:28802043"
FT TRANSMEM 384..404
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 405..455
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:28802043"
FT TRANSMEM 456..476
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 477..479
FT /note="Extracellular"
FT /evidence="ECO:0000305|PubMed:28802043"
FT REGION 261..280
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 167
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 479 AA; 54426 MW; 8964D26B6FE1DB5A CRC64;
MMKMKQQGLV ADLLPNIRVM KFFGHFVFNY YDDNSSKYLH KIFCCVNLFL LLLQFALCAV
NLIIESADVD DLTANTITLL FFTHSIVKII YFAVRSKYFY RTWAIWNNPN SHPLFAESNA
RYHAIALKKM RLLLFLVGAT TVLSAIAWTV LTFFEHPIRK LVDPVTNETT IIELPQLLLR
SYYPFDASKG IMHVIVLIYQ FYWVLFMLID ANSLDVLFCS WLLFACEQLQ HLKQIMKPLM
ELSATLDTVV PNSSELFKAG SAEHLRESEN QPPPPVPPQG DSMLDLDLRN IYSNRQDFTA
TFRPTAGMTF NGGVGPNGLT KKQEMLVRSA IKYWVERHKH IVRLVTAVGD AYGFALLLHM
LTTTITLTLL AYQATKVNGV NVYAASTIGY IIYTFGQVFL FCIFGNRLIE ESTSVMEAAY
SCHWYDGSEE AKTFVQIVCQ QCQKAMSISG AKFFTVSLDL FASVLGAVVT YFMVLVQLK
