ORCT_DROME
ID ORCT_DROME Reviewed; 548 AA.
AC Q9VCA2; O01383; O01384;
DT 19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 154.
DE RecName: Full=Organic cation transporter protein;
GN Name=Orct; ORFNames=CG6331;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Larva;
RX PubMed=9409773; DOI=10.1016/s0378-1119(97)00429-0;
RA Taylor C.A.M., Stanley K., Shirras A.D.;
RT "The Orct gene of Drosophila melanogaster codes for a putative organic
RT cation transporter with six or 12 transmembrane domains.";
RL Gene 201:69-74(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-97, AND IDENTIFICATION BY MASS
RP SPECTROMETRY.
RX PubMed=19349973; DOI=10.1038/nbt.1532;
RA Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M.,
RA Schiess R., Aebersold R., Watts J.D.;
RT "Mass-spectrometric identification and relative quantification of N-linked
RT cell surface glycoproteins.";
RL Nat. Biotechnol. 27:378-386(2009).
CC -!- FUNCTION: Probably transports organic cations. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in embryos and adults at low level.
CC Expressed at higher level in third instar larvae.
CC -!- SIMILARITY: Belongs to the major facilitator (TC 2.A.1) superfamily.
CC Organic cation transporter (TC 2.A.1.19) family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA73030.1; Type=Miscellaneous discrepancy; Note=Chimeric cDNA.; Evidence={ECO:0000305};
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DR EMBL; Y12399; CAA73030.1; ALT_SEQ; mRNA.
DR EMBL; Y12400; CAA73031.1; -; mRNA.
DR EMBL; AE014297; AAF56271.1; -; Genomic_DNA.
DR EMBL; AY058437; AAL13666.1; -; mRNA.
DR RefSeq; NP_001262908.1; NM_001275979.1.
DR RefSeq; NP_524479.1; NM_079755.2.
DR AlphaFoldDB; Q9VCA2; -.
DR SMR; Q9VCA2; -.
DR BioGRID; 67819; 1.
DR IntAct; Q9VCA2; 1.
DR STRING; 7227.FBpp0083983; -.
DR TCDB; 2.A.1.19.36; the major facilitator superfamily (mfs).
DR GlyGen; Q9VCA2; 4 sites.
DR iPTMnet; Q9VCA2; -.
DR PaxDb; Q9VCA2; -.
DR PRIDE; Q9VCA2; -.
DR DNASU; 42891; -.
DR EnsemblMetazoa; FBtr0084599; FBpp0083983; FBgn0019952.
DR EnsemblMetazoa; FBtr0334870; FBpp0306893; FBgn0019952.
DR GeneID; 42891; -.
DR KEGG; dme:Dmel_CG6331; -.
DR CTD; 42891; -.
DR FlyBase; FBgn0019952; Orct.
DR VEuPathDB; VectorBase:FBgn0019952; -.
DR eggNOG; KOG0255; Eukaryota.
DR GeneTree; ENSGT00940000167832; -.
DR HOGENOM; CLU_001265_33_4_1; -.
DR InParanoid; Q9VCA2; -.
DR OMA; VQMFFSV; -.
DR OrthoDB; 704438at2759; -.
DR PhylomeDB; Q9VCA2; -.
DR Reactome; R-DME-112311; Neurotransmitter clearance.
DR Reactome; R-DME-181430; Norepinephrine Neurotransmitter Release Cycle.
DR Reactome; R-DME-197264; Nicotinamide salvaging.
DR Reactome; R-DME-200425; Carnitine metabolism.
DR Reactome; R-DME-2161517; Abacavir transmembrane transport.
DR Reactome; R-DME-442660; Na+/Cl- dependent neurotransmitter transporters.
DR Reactome; R-DME-549127; Organic cation transport.
DR Reactome; R-DME-561048; Organic anion transport.
DR Reactome; R-DME-9749641; Aspirin ADME.
DR BioGRID-ORCS; 42891; 0 hits in 3 CRISPR screens.
DR ChiTaRS; Orct; fly.
DR GenomeRNAi; 42891; -.
DR PRO; PR:Q9VCA2; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0019952; Expressed in saliva-secreting gland and 34 other tissues.
DR ExpressionAtlas; Q9VCA2; baseline and differential.
DR Genevisible; Q9VCA2; DM.
DR GO; GO:0016021; C:integral component of membrane; NAS:UniProtKB.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0006915; P:apoptotic process; TAS:UniProtKB.
DR GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR005828; MFS_sugar_transport-like.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF00083; Sugar_tr; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 1: Evidence at protein level;
KW Glycoprotein; Ion transport; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..548
FT /note="Organic cation transporter protein"
FT /id="PRO_0000220514"
FT TOPO_DOM 1..22
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 23..43
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 44..127
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 128..148
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 149..158
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 159..179
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 180..189
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 190..210
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 211..219
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 220..240
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 241..244
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 245..265
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 266..337
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 338..358
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 359..366
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 367..387
FT /note="Helical; Name=8"
FT /evidence="ECO:0000255"
FT TOPO_DOM 388..395
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 396..416
FT /note="Helical; Name=9"
FT /evidence="ECO:0000255"
FT TOPO_DOM 417..419
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 420..440
FT /note="Helical; Name=10"
FT /evidence="ECO:0000255"
FT TOPO_DOM 441..453
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 454..474
FT /note="Helical; Name=11"
FT /evidence="ECO:0000255"
FT TOPO_DOM 475..482
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 483..503
FT /note="Helical; Name=12"
FT /evidence="ECO:0000255"
FT TOPO_DOM 504..548
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 512..548
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 533..548
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 55
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 67
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 89
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 97
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:19349973"
FT CONFLICT 375
FT /note="A -> P (in Ref. 1; CAA73031)"
FT /evidence="ECO:0000305"
FT CONFLICT 385
FT /note="L -> F (in Ref. 1; CAA73031)"
FT /evidence="ECO:0000305"
FT CONFLICT 501
FT /note="L -> R (in Ref. 1; CAA73031)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 548 AA; 61003 MW; 08D7F97599B477AF CRC64;
MGYDDVITHL GEFGPYQKRI YYLLCLPAIV CAFHKLAGVF LLAKPDFRCA LPYENGSIYE
LSPHLWNLSY PENERCSYYD VDYTEEYLNG SIPRSSNETK TCSSYVYDRS KYLNSAVTEW
NLVCSRSLLS ATSDSLFMLG VLLGSLIFGQ MSDKLGRKPT FFASLVLQLI FGVLAAVAPE
YFSYTISRMI VGATTSGVFL VAYVIALEMV GSSYRLFAGV AMQMFFSVGF MLTAGFAYFI
HDWRWLQIAI TLPGLLFLCY YWIIPESARW LLMKGRKDEA FVIIEKAAKE NKVEVPNEIY
EQLVDEVAEK KKQDEMAASQ PAATVFDLLR YPNLRRKTLL IFFDWFVNSG VYYGLSWNTN
NLGGNQLVNF MISGAVEIPG YTLLLFTLNR WGRRSILCGT MMVAGISLLA TIFVPSDMNW
LIVACAMIGK LAITSSYGTI YIFSAEQFPT VVRNVGLGAS SMVARVGGIL APYLKLLGEI
WRPLPLIICG ALSLTAGLLS LLLPETLNKP MPETIEDGEN FGKKPAPQET AEEGGTQELS
GMLNGKSG
